Pronase Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, ID, IF, IP, R, WB |
Recommended Dilution | Can be used for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice. Working dilutions: vary widely, but may be between 1/1000 and 1/20000. |
Reactivities | Streptomyces griseus |
Host | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Pronase isolated and purified from Streptomyces griseus. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Specificity | IgG fraction of polyclonal rabbit antiserum to pronase from Streptomyces griseus. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. |
Formulation | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized Hyperimmune IgG fraction |
Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
Conjugation | Unconjugated |
Storage | Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Synonyms | Streptogrisin-A, Streptogrisin-B, Serine protease A, Serine protease B, SGPA, SGPB, sprA, sprB |
Reference Data |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.