NFAT1 (NFATC2) pSer330 Rabbit Polyclonal Antibody

CAT#: AP12851PU-N

NFAT1 (NFATC2) pSer330 rabbit polyclonal antibody, Aff - Purified

Product Images

Dot blot analysis of anti-NFATC2-pSer330 Phospho-specific Pab (Cat.#AP12851PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Specifications

Product Data

• Recommended Dilution: ELISA: 1/1,000.
  Dot Blot: 1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S330 of human NFATC2.
• Specificity: This antibody detects NFATC2 pSer330.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: nuclear factor of activated T-cells 2
• Database Link:
  Entrez Gene 4773 Human
  UniProt ID Q13469
• Background: NFATC2 is a member of the nuclear factor of activated T cells (NFAT) family. It is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response.
• Synonyms: NFATC2, NFAT-1, NFATP
• Note: Molecular weight: 99654 Da