PTP1B (PTPN1) Rabbit Polyclonal Antibody

CAT#: AP06575PU-N

PTP1B (PTPN1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PTP1B antibody in extracts from COS7 cells treated with UV 30'.

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  Immunohistochemistry (IHC) analyzes of PTP1B antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 20-70 of Human PTP1B.
• Specificity: This antibody detects endogenous levels of PTP1B protein.
  (region surrounding Tyr46)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 49 kDa
• Gene Name: protein tyrosine phosphatase, non-receptor type 1
• Database Link:
  Entrez Gene 5770 Human
  UniProt ID P18031
• Background: The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosinephosphatases. These latter enzymes bear little resemblance to either theprotein serine and protein threonine phosphatases or to the acid and alkalinephosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein of approximately 40 kDa molecular weight. On the basis of sequence analysis, PTP1B expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, ahomolog of the neural adhesion molecule (NCAM). PTPase 1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.
• Synonyms: Protein Tyrosin Phosphatase , PTP1B, PTP-1B