Cytokeratin 5 Mouse Monoclonal Antibody [Clone ID: AT12C4]

CAT#: AM50628PU-N

Cytokeratin 5 mouse monoclonal antibody, clone AT12C4, Purified

Product Images

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : A431 cell lysate

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  Immunohistochemistry: Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT5 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

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  Immunofluorescence: ICC/IF analysis of KRT5 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Specifications

Product Data

• Clone Name: AT12C4
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot.
  Flow cytometry:
  Immunofluorescence/Immnunocytochemistry.
  Immunohistochemistry on Paraffin Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human KRT5 (1-590aa) purified from E. coli.
• Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 5
• Database Link:
  Entrez Gene 3852 Human
  UniProt ID P13647
• Background: Keratin 5, also known as KRT5, K5, or CK5, dimerizes with keratin 14 and forms the intermediate filaments(IF) that make up the cytoskeleton of basal epithelial cells. This protein is involved in several diseases including epidermolysis bullosa simplex and breast and lung cancers. Keratin 5(and K14) are expressed primarily in basal keratinocytes in the epidermis, specifically in the stratified epithelium lining the skin and digestive tract. Keratin intermediate filaments make up the cytoskeletal scaffold within epithelial cells, which contributes to the cell architecture and provides the cells with the ability to withstand mechanical, and non-mechanical, stresses. K5/K14 keratin pairs are able to undergo extensive bundling due to the non-helical tail of K15 acting as a weak cross-linker at the intermediate filament surface. This bundling increase the elasticity, and therefore the mechanical resilience, of the intermediate filaments.
• Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5