Liver Diseases

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FXN - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

FXN - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin

FXN (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
SR320201 is the updated version of SR301654.

FXN HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T

Lenti ORF particles, FXN (Myc-DDK-tagged)-Human frataxin (FXN), nuclear gene encoding mitochondrial protein, transcript variant 2, 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Buy this product and get 50% off on the Lenti RapidTiter kit. Use Code: Rapid50

Lenti ORF particles, FXN (mGFP-tagged)-Human frataxin (FXN), nuclear gene encoding mitochondrial protein, transcript variant 2, 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Buy this product and get 50% off on the Lenti RapidTiter kit. Use Code: Rapid50

Frataxin (FXN) (42-210, His-tag) human recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

Frataxin (FXN) (42-210, His-tag) human recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

FXN CRISPRa kit - CRISPR gene activation of human frataxin

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

FXN HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T

FXN - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin

qPCR primer pairs and template standards against Homo sapiens gene FXN

Application Plasmid of exact quantity for transcript copy number calculation

qSTAR qPCR primer pairs against Homo sapiens gene FXN

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)

3' UTR clone of frataxin (FXN) nuclear gene encoding mitochondrial protein transcript variant 3 for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

FXN (untagged)-Human frataxin (FXN), nuclear gene encoding mitochondrial protein, transcript variant 2

Vector pCMV6 series
Tag Tag Free