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Proteins
Transient overexpression of CFAP74 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
| Applications | IHC |
| Other Names | C1orf222; KIAA1751 |
| Accession Number | NM_001080484, NP_001073953 |
Transient overexpression of E2F5, transcript variant 2, in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
| Applications | IHC |
| Other Names | E2F-5 |
| Accession Number | NM_001083588, NP_001077057 |
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Applications
Conjugates
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Tags
- C-Myc/DDK (77393)
- C-MYC/DDK (26612)
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- No (1)
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- E. coli (1936)
- Human (1446)
- Human 293 (126)
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Mutants
QC Levels
Families
- Druggable Genome (40038)
- WB (19632)
- Transmembrane (19015)
- Transcription Factors (9044)
- Secreted Protein (8367)
- Protein Kinase (3386)
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- Ion Channels: Other (651)
- Adult stem cells (632)
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- Nuclear Hormone Receptor (413)
- Cancer stem cells (312)
- Induced pluripotent stem cells (288)
- P450 (281)
- Ion Channels: Potassium (214)
- Stem cell relevant signaling - JAK/STAT signaling pathway (206)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (167)
- Stem cell relevant signaling - Wnt Signaling pathway (130)
- Stem cell relevant signaling - DSL/Notch pathway (111)
- Ion Channels: Cys-loop Receptors (106)
- Ion Channels: Transient receptor potential (63)
- Ion Channels: Glutamate Receptors (57)
- Ion Channels: ATP Receptors (44)
- Ion Channels: Sodium (28)
- Ion Channels: Cyclic nucleotide gated (14)
- Suitable for use in ELISA and Western blots. (10)
- Ion Channels: Calcium (9)
- Suitable for use in ELISA and Western blot. (8)
- Suitable for ELISA and Western blot. (5)
-
ELISA.
Western Blot. (4) - Serological studies of influenza A virus (4)
- Suitable for use in ELISA (4)
- Suitable for use in ELISA. (4)
- immunogen for antibody production. (4)
-
immunogen for antibody production.
Has been tested with MAb to Influenza A in ELISA. (4) - ELISA (3)
-
ELISA.
Western Blot. (3) - Serological studies of Influenza B virus (3)
- 000. (2)
-
ELISA.
Lateral Flow. (2) - ELISA. (2)
-
ELISA.
Western Blot. (2) - Colloidal Gold and Latex Beads. (2)
- ELISA and Western blot. (2)
- ELISA. (2)
-
ELISA.
Western blot.
Lateral flow. (2) -
ELISA: 0.5-1.0 µg/ml coating concentration.
After thawing (2) - Immunogen (2)
- Recommended for use in standard solid phase immunoassays. (2)
- Serological studies of Influenza A virus (2)
- Serological studies of influenza B virus (2)
- Suitable in ELISA (2)
- Suitable in Western Blot with a suggested dilution of 1:1 (2)
- Use in standard solid phase immunoassays. (2)
- Western blot (2)
- Western blot and Immunochromatographic assays. (2)
- Western blot and immunochromatographic assays. (2)
- mix thoroughly by vortexing before use. (2)
- 000 and 30 (1)
- 000 and 50 (1)
- 000 daltons and a doublet band at approximately 28 (1)
- 000 daltons that are not glycosylated. (1)
- 000 or less. This should be performed in a cold room. (1)
- 000) and Immunohistochemistry (1/200-1/1 (1)
-
000) as well as other phosphatase-Biotin/biotin based enzymatic assays.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin. (1) - 000-1/32 (1)
- 1/500-1/2 (1)
- 10 mM Tris-HCl (1)
- 2 mM dithiothreitol (1)
- 500) (1)
-
ELISA (0.5 µg/ml).
Western blot. (1) -
ELISA and Western blot.
Using an antigen Capture ELISA (1) -
ELISA.
Western blot. (1) -
ELISA.
Western Blot.
Lateral Flow. (1) -
ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting. (1) -
ELISA.
Western blot: 1/1000.
Colloidal Gold and Latex Beads. (1) - ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays. (1)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (1) -
RIA.
Western blot. (1) - Antigen in ELISA and Western blots. (1)
- Apo AI can be renatured into PBS (1)
-
Colloidal Gold and Western Blot.
Not recommended for Latex Beads. (1) - ELISA (1/8 (1)
- ELISA and Western blot Immunoassay (1)
-
ELISA and Western blot.
Glycosylation is heterogeneous and results in several immunoreactive products on Western blots that range from approximately 40 (1) -
ELISA.
Western blot. (1) -
ELISA: 0.4-0.8 µg/well.
After thawing (1) - FACS (1)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization. (1) -
Second order rate constants for inhibition of:
uPA = 6.3 x 10e6 M-1s-1
tPA = 5.7 x 10e6 M-1s-1 (1) - Should be sonicated immediately prior to use to ensure that the preparation is uniform. Used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases and M capture formats. (1)
- Specific methodologies have not been tested using this product. (1)
- Suitable for ELISA (1)
- Suitable for ELISA. (1)
- Suitable for Immunoblotting (Western or Dot blot (1)
- Suitable for use as a positive control in Lateral Flow assays and when spiked into stool samples. (1)
-
Suitable for use in EIA and Western Blot. Each laboratory should determine an optimum working titer for
use in its particular application. (1) - Suitable for use in ELISA and Western blot (1)
- Suitable for use in ELISA and Western blot immunoassay. (1)
- Suitable for use in Western blot. (1)
- Suitable in ELISA. (1)
- Suitable to promote the adhesion and propagation of cells in vitro when used to coat cell culture surfaces (1)
- TBS and other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14 (1)
- Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies. (1)
- Tested in ELISA with anti-HBsAg antibodies. (1)
- The DYKDDDDK peptide (Flag) is used as a fusion tag for immunoaffinity chromatography. (1)
- The antigen preparation should be sonicated immediately prior to use to ensure that the preparation is uniform. Grade 2 antigen is suitable for use for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases. This preparation may be further purified to meet the needs of a particular assay format. (1)
- This preparation may be used as is in a variety of immunoassay formats or may be further purified to meet the needs of a particular assay format. May be used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases. (1)
-
Variety of Immunoassay formats including Microwell ELISA.
This product should be sonicated immediately prior to use to ensure that the preparation is uniform. (1) - Western Blot and ELISA. (1)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (1)
- calibrator in immunoassays and in vitro studies. (1)
- calibrator in immunoassays and in vitro studies. (1)
- completely thaw the tube at 37°C without agitation (1)
- e.g. Dulbecco's PBS). (1)
- each lot is tested against a reference material for both IGM and IgG activity. (1)
- flicking or mixing (1)
- glassware and microcarrier beads. After spin and opening of the vial (1)
- immunogen for antibody production. Has been tested with anti-influenza A monoclonal antibodies in ELISA. (1)
- immunogen for antibody production. Has been tested with anti-influenza B monoclonal antibodies in ELISA. (1)
- immunogen for antibody production. Has been tested with anti-influenza B monoclonal antibodies in ELISA. (1)
- including plasticware (1)
- mix thoroughly by vortexing before use! (1)
-
pH 7.4).
For immunization purposes (1) - the solution can be further dialyzed against PBS (phosphate buffered saline (1)
- then gently mix the contents with a pipette. (1)
Pathways
- Metabolic pathways (8524)
- Pathways in cancer (3306)
- Cytokine-cytokine receptor interaction (2718)
- MAPK signaling pathway (2683)
- Regulation of actin cytoskeleton (1900)
- Focal adhesion (1830)
- Chemokine signaling pathway (1642)
- Endocytosis (1599)
- Jak-STAT signaling pathway (1423)
- Neurotrophin signaling pathway (1399)
- Purine metabolism (1328)
- Natural killer cell mediated cytotoxicity (1267)
- Alzheimer's disease (1253)
- Ubiquitin mediated proteolysis (1224)
- Wnt signaling pathway (1207)
- Insulin signaling pathway (1198)
- Huntington's disease (1184)
- T cell receptor signaling pathway (1173)
- Axon guidance (1103)
- Hematopoietic cell lineage (1091)
- Toll-like receptor signaling pathway (1087)
- Cell cycle (1085)
- Cell adhesion molecules (CAMs) (1081)
- Calcium signaling pathway (1067)
- Neuroactive ligand-receptor interaction (1047)
- Leukocyte transendothelial migration (1031)
- Tight junction (1023)
- ErbB signaling pathway (986)
- Oocyte meiosis (973)
- Progesterone-mediated oocyte maturation (970)
- Prostate cancer (937)
- Apoptosis (921)
- Lysosome (906)
- Spliceosome (894)
- GnRH signaling pathway (876)
- Pancreatic cancer (873)
- Pyrimidine metabolism (859)
- Adherens junction (834)
- Colorectal cancer (831)
- Fc gamma R-mediated phagocytosis (828)
- Melanoma (814)
- Parkinson's disease (805)
- Fc epsilon RI signaling pathway (802)
- Systemic lupus erythematosus (787)
- Antigen processing and presentation (779)
- Hypertrophic cardiomyopathy (HCM) (771)
- Dilated cardiomyopathy (770)
- Renal cell carcinoma (767)
- TGF-beta signaling pathway (765)
- Melanogenesis (751)
- VEGF signaling pathway (746)
- B cell receptor signaling pathway (744)
- Chronic myeloid leukemia (736)
- Small cell lung cancer (724)
- Vascular smooth muscle contraction (720)
- Glioma (709)
- NOD-like receptor signaling pathway (694)
- RIG-I-like receptor signaling pathway (692)
- Oxidative phosphorylation (691)
- Gap junction (682)
- Glycolysis / Gluconeogenesis (682)
- Adipocytokine signaling pathway (673)
- PPAR signaling pathway (670)
- p53 signaling pathway (661)
- Epithelial cell signaling in Helicobacter pylori infection (659)
- Complement and coagulation cascades (645)
- Acute myeloid leukemia (631)
- Long-term potentiation (605)
- Pathogenic Escherichia coli infection (580)
- Non-small cell lung cancer (576)
- Phosphatidylinositol signaling system (575)
- Endometrial cancer (572)
- Olfactory transduction (569)
- Long-term depression (556)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (550)
- Cytosolic DNA-sensing pathway (547)
- Viral myocarditis (542)
- Arginine and proline metabolism (540)
- Bladder cancer (535)
- Amyotrophic lateral sclerosis (ALS) (530)
- Metabolism of xenobiotics by cytochrome P450 (520)
- Drug metabolism - cytochrome P450 (513)
- Cardiac muscle contraction (512)
- Ribosome (507)
- mTOR signaling pathway (503)
- ECM-receptor interaction (502)
- Glycerophospholipid metabolism (473)
- Amino sugar and nucleotide sugar metabolism (457)
- Glutathione metabolism (457)
- Valine (452)
- Autoimmune thyroid disease (446)
- Type I diabetes mellitus (437)
- Inositol phosphate metabolism (434)
- Vibrio cholerae infection (423)
- Drug metabolism - other enzymes (415)
- Thyroid cancer (408)
- Type II diabetes mellitus (403)
- leucine and isoleucine degradation (402)
- Proteasome (399)
- RNA degradation (397)
- Retinol metabolism (394)
- Tyrosine metabolism (387)
- Hedgehog signaling pathway (385)
- Fructose and mannose metabolism (375)
- Graft-versus-host disease (370)
- Pyruvate metabolism (370)
- Starch and sucrose metabolism (366)
- Fatty acid metabolism (362)
- Allograft rejection (354)
- Arachidonic acid metabolism (354)
- Tryptophan metabolism (351)
- Prion diseases (345)
- Alanine (341)
- aspartate and glutamate metabolism (341)
- Cysteine and methionine metabolism (339)
- Glycerolipid metabolism (334)
- Basal cell carcinoma (328)
- Nucleotide excision repair (321)
- Base excision repair (320)
- Primary immunodeficiency (314)
- Pentose phosphate pathway (304)
- Regulation of autophagy (302)
- SNARE interactions in vesicular transport (296)
- Butanoate metabolism (293)
- Porphyrin and chlorophyll metabolism (292)
- Androgen and estrogen metabolism (287)
- Lysine degradation (285)
- Nitrogen metabolism (280)
- Notch signaling pathway (272)
- Sphingolipid metabolism (272)
- Citrate cycle (TCA cycle) (266)
- Propanoate metabolism (265)
- Galactose metabolism (263)
- DNA replication (256)
- Aminoacyl-tRNA biosynthesis (255)
- Histidine metabolism (249)
- Glycine (247)
- serine and threonine metabolism (247)
- Asthma (245)
- Ether lipid metabolism (221)
- Nicotinate and nicotinamide metabolism (218)
- beta-Alanine metabolism (217)
- Selenoamino acid metabolism (214)
- Taste transduction (208)
- Dorso-ventral axis formation (205)
- N-Glycan biosynthesis (205)
- RNA polymerase (201)
- Maturity onset diabetes of the young (195)
- Phenylalanine metabolism (195)
- Linoleic acid metabolism (192)
- Glycosphingolipid biosynthesis - lacto and neolacto series (190)
- Basal transcription factors (184)
- Homologous recombination (183)
- Sulfur metabolism (182)
- Glycosaminoglycan degradation (171)
- Mismatch repair (170)
- Pantothenate and CoA biosynthesis (161)
- Pentose and glucuronate interconversions (160)
- Riboflavin metabolism (159)
- O-Glycan biosynthesis (153)
- Glyoxylate and dicarboxylate metabolism (151)
- Terpenoid backbone biosynthesis (151)
- Other glycan degradation (148)
- Renin-angiotensin system (143)
- Ascorbate and aldarate metabolism (138)
- Primary bile acid biosynthesis (137)
- One carbon pool by folate (135)
- Biosynthesis of unsaturated fatty acids (116)
- alpha-Linolenic acid metabolism (116)
- Heparan sulfate biosynthesis (112)
- Glycosphingolipid biosynthesis - globo series (111)
- C21-Steroid hormone metabolism (107)
- Folate biosynthesis (105)
- Limonene and pinene degradation (105)
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis (102)
- Keratan sulfate biosynthesis (101)
- Non-homologous end-joining (90)
- Chondroitin sulfate biosynthesis (86)
- Circadian rhythm - mammal (86)
- Steroid biosynthesis (86)
- Thiamine metabolism (86)
- Synthesis and degradation of ketone bodies (83)
- ABC transporters (81)
- Taurine and hypotaurine metabolism (81)
- leucine and isoleucine biosynthesis (81)
- Glycosphingolipid biosynthesis - ganglio series (80)
- Caffeine metabolism (67)
- Fatty acid elongation in mitochondria (65)
- Methane metabolism (64)
- Protein export (56)
- Ubiquinone and other terpenoid-quinone biosynthesis (56)
- Cyanoamino acid metabolism (52)
- Fatty acid biosynthesis (40)
- Phenylalanine (40)
- tyrosine and tryptophan biosynthesis (40)
- Vitamin B6 metabolism (36)
- Lysine biosynthesis (30)
- D-Glutamine and D-glutamate metabolism (28)
- Lipoic acid metabolism (19)
- Biotin metabolism (14)
- O-Mannosyl glycan biosynthesis (13)
- D-Arginine and D-ornithine metabolism (11)