DYKDDDDK Epitope Tag chicken polyclonal antibody, Aff - Purified
Applications | ELISA, IF, IHC, IP, WB |
Conjugation | Unconjugated |
Immunogen | Synthetic peptide containing the DYKDDDDK epitope conjugated to Keyhole Limpet Hemocyanin (KLH). Production: The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against Phosphate-Buffered isotonic Saline. Sodium azide was added, the protein concentration was adjusted and the antibodies were filter-sterilized (0.2 μm). |