TRAF6 (NM_004620) Human Recombinant Protein
SKU
TP319528
Recombinant protein of human TNF receptor-associated factor 6 (TRAF6), transcript variant 2, 20 µg
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 59.4 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | Enzyme substrate (PMID: 26221041) Pull-down assay (PMID: 26839314) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004611 |
| Locus ID | 7189 |
| UniProt ID | Q9Y4K3 |
| Cytogenetics | 11p12 |
| RefSeq Size | 2515 |
| RefSeq ORF | 1566 |
| Synonyms | MGC:3310; RNF85 |
| Summary | The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. This protein also interacts with the transforming growth factor (TGF) beta receptor complex and is required for Smad-independent activation of the JNK and p38 kinases. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. Two alternatively spliced transcript variants, encoding an identical protein, have been reported. provided by RefSeq, Feb 2012 |
| Protein Categories | Cytokines, Growth Factors, Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, Toll-like receptor signaling pathway, Ubiquitin mediated proteolysis |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "TRAF6" proteins (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH319528 | TRAF6 MS Standard C13 and N15-labeled recombinant protein (NP_004611) | 10 ug |
$3,360.00
|
|
| LC401463 | Lyophilized TRAF6 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC403440 | Lyophilized TRAF6 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401463 | Transient overexpression lysate of TNF receptor-associated factor 6 (TRAF6), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY403440 | Transient overexpression lysate of TNF receptor-associated factor 6 (TRAF6), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
Citations
TRAF6 function as a novel co-regulator of Wnt3a target genes in prostate cancer
Aripaka, Gudey, Zang et al
EBioMedicine (2019) 45, 192-207 DOI: 10.1016/j.ebiom.2019.06.046
Aripaka, Gudey, Zang et al
EBioMedicine (2019) 45, 192-207 DOI: 10.1016/j.ebiom.2019.06.046
AI Summary: In this study, recombinant human TRAF6-Flag protein from OriGene (#TP319528) was used to investigate the role of TRAF6 in Wnt3a signaling and its regulation of Wnt target genes in prostate cancer.
Snippet: … Expression of LRP51407–1615HA-(His)6 WT was performed in human embryonic kidney (HEK293) cells and purified by Ni-NTA chromatography. Recombinant human TRAF6-Flag was purchased from Origene, USA (#:TP319528). 2.6 Dual luciferase assay Wnt signaling was investigated by TCF reporter plasmid TOPflash transiently transfected into PC3U and SW480 cell lines along with either siTRAF6 o;... … Expression of LRP51407–1615HA-(His)6 WT was performed in human embryonic kidney (HEK293) cells and purified by Ni-NTA chromatography. Recombinant human TRAF6-Flag was purchased from Origene, USA (#:TP319528). 2.6 Dual luciferase assay Wnt signaling was investigated by TCF reporter plasmid TOPflash transiently transfected into PC3U and SW480 cell lines along with either siTRAF6 o;Expression of LRP5 1407-1615 HA-(His) 6 WT was performed in human embryonic kidney (HEK293) cells and purified by Ni-NTA chromatography. Recombinant human TRAF6-Flag was purchased from Origene, USA (#:TP319528). 2.6. …A synthetic gene encoding for the intracellular domain of human LRP5 from amino acid 1407 until 1615 was ordered from Eurofinsgenomics (Ebersberg, Germany) as wild type LRP51407–1615WT or with a mutant TRAF6 consensus binding motif LRP51407–1615 E1425A and further cloned into the eukaryotic expression vector pCMVtag5 upstream of the HA and (His)6 tag. Expression of LRP51407–1615HA-(His)6 WT was performed in human embryonic kidney (HEK293) cells and purified by Ni-NTA chromatography. Recombinant human TRAF6-Flag was purchased from Origene, USA (#:TP319528)
TRIM59 regulates autophagy through modulating both the transcription and the ubiquitination of BECN1
Han, Guo, Gan et al
Autophagy (2018) 14 (12), 2035-2048 DOI: 10.1080/15548627.2018.1491493
Han, Guo, Gan et al
Autophagy (2018) 14 (12), 2035-2048 DOI: 10.1080/15548627.2018.1491493
AI Summary: In this study, the OriGene product pCMV6-AC-GFP-TRIM59 plasmid (PS100010) was used to investigate the role of TRIM59 in regulating autophagy through its effects on BECN1 transcription and ubiquitination.
Snippet: … RESEARCH PAPER - BASIC SCIENCE TRIM59 regulates autophagy through modulating both the transcription and the ubiquitination of BECN1 Tianyu Hana,b, Meng Guoa, Mingxi Gana, Bentong Yuc, Xiaoli Tianb, and Jian-Bin Wanga;The plasmid pcDNA3.1-FLAG-PIK3C3 was kindly provided by Dr. Qimin Zhan (Chinese Academy of Medical Sciences and Peking Union Medical College). The plasmid pCMV6-AC-GFP-TRIM59 was purchased from OriGene (PS100010). The mutant plasmids (BECN1-K117R, BECN1-E299A) were made by Generay Biotech
Ubiquitin-specific protease 20 Regulates the Reciprocal Functions of Beta-arrestin2 in Toll-like Receptor 4-promoted NFkappaB Activation
Jean-Charles, Zhang, Wu et al
J. Biol. Chem. (2016)
Jean-Charles, Zhang, Wu et al
J. Biol. Chem. (2016)
Dynamic arginine methylation of TNF receptor associated factor 6 regulates Toll-like receptor signaling
Tikhanovich, Kuravi, Artigues et al
J. Biol. Chem. (2015)
Tikhanovich, Kuravi, Artigues et al
J. Biol. Chem. (2015)
JBC Papers in Press. Published on February 2, 2016 as Manuscript M115. 687129
Jean-Charles, Zhang, Wu et al
Journal of Biological Chemistry (2016) 291 (14)
Jean-Charles, Zhang, Wu et al
Journal of Biological Chemistry (2016) 291 (14)
Showing 1-5 of 5 papers.
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