B Raf(BRAF) (NM_004333) Human Recombinant Protein

CAT#: TP311013

Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF)

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 Product Datasheet for 'TP311013'

USD 680


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Size
    • 20 ug

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Specifications

Product Data
Description Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF)
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC211013. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag C-MYC/DDK
Predicted MW 84.3 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bioactivity BRAF kinase activity was measured in an HTRF® assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader.
Reference Data
RefSeq NP_004324
Locus ID 673
Refseq Size 2477
Cytogenetics 7q34
Refseq ORF 2298
Synonyms B-raf; B-RAF1; BRAF1; NS7; RAFB1
Summary This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008].
Protein Families
Protein Pathways
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