Glucose-6-phosphate isomerase (GPI) (1-558, His-tag)

USD 400.00


Availability*
2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Species Human
Expression Host E. coli
Tag His-tag
Predicted MW 65.3 kDa
Concentration 1.0 mg/ml (determined by bradford assay)
Purity > 95 % by SDS - PAGE
Buffer 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
Bioactivity Specific activity is > 400 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will convert 1.0 umole of D-Fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 37C.
Endotoxin < 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation Liquid purified protein
Reference Data
RefSeq NP_000166
Locus ID 2821
Cytogenetics 19q13.11
Synonyms AMF; GNPI; NLK; PGI; PHI; SA-36; SA36
Summary This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Protein Families Druggable Genome
Protein Pathways Amino sugar and nucleotide sugar metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism

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68 Mouse Clones