Desmin Human Protein
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Other products for "DES"
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Predicted MW | 53,539 |
Concentration | lot specific |
Purity | >95% determined by SDS gelelectrophoresis |
Presentation | Purified |
Buffer | Presentation State: Purified State: Lyophilised purified protein Buffer System: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride. Reconstitute with 70 µl distilled water (final volume 100 µl) |
Preparation | Lyophilised purified protein |
Applications | Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). |
Protein Description | Recombinant human desmin |
Note | Isoelectric Point pI 5.16 (calculated from sequence) |
Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
RefSeq | NP_001918 |
Locus ID | 1674 |
UniProt ID | P17661, Q53SB5 |
Cytogenetics | 2q35 |
Synonyms | CDCD3; CSM1; CSM2; LGMD1D; LGMD1E; LGMD2R |
Summary | This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008] |
Protein Families | Druggable Genome |
Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM) |
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