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Desmin Human Protein

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SKU
AR09361PU-S
Desmin human recombinant protein, 0.1 mg
Size:
100 ug
  $550.00
2 Weeks*
Bulk/Customize

Other products for "DES or NM_001927"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Predicted MW 53,539
Concentration lot specific
Purity >95% determined by SDS gelelectrophoresis
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilised purified protein
Buffer System: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride.
Reconstitute with 70 µl distilled water (final volume 100 µl)
Preparation Lyophilised purified protein
Recommended Dilution Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
Protein Description Recombinant human desmin
Note Isoelectric Point pI 5.16 (calculated from sequence)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_001918
Locus ID 1674
UniProt ID P17661
Cytogenetics 2q35
Synonyms DES
Summary This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. provided by RefSeq, Jul 2008
Protein Categories Cardiovascular diseases, Intracellular Proteins
Protein Families 10 mM Tris-HCl, 2 mM dithiothreitol, e.g. Dulbecco's PBS)., pH 7.4). For immunization purposes, Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl, the solution can be further dialyzed against PBS (phosphate buffered saline
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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