PTEN (1-403, His-tag)

CAT#: AR09238PU-S

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Recombinant PTEN protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.


 Product Datasheet for 'AR09238PU-S'

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USD 320.00


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2 Weeks

Size
    • 20 ug


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Product Data
Description Recombinant PTEN protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
Species Human
Expression Host E. coli
Tag His-tag
Predicted MW 49.3 kDa
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity > 85 % by SDS - PAGE
Buffer 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM EDTA, 2 mM DTT, 100 mM NaCl, and 20% glycerol
Preparation Liquid purified protein
Reference Data
RefSeq NP_000305
Locus ID 5728
Cytogenetics 10q23.31
Synonyms 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; PTENbeta; TEP1
Summary This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]
Protein Families Druggable Genome, Phosphatase
Protein Pathways Endometrial cancer, Focal adhesion, Glioma, Inositol phosphate metabolism, Melanoma, p53 signaling pathway, Pathways in cancer, Phosphatidylinositol signaling system, Prostate cancer, Small cell lung cancer, Tight junction

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68 Mouse Clones