ASCIZ (ATMIN) (NM_001300728) Human Untagged Clone

CAT#: SC337159

ATMIN (untagged) - Human ATM interactor (ATMIN), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASCIZ
• Synonyms: ASCIZ; ZNF822
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC337159 representing NM_001300728.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAAATGCATGCTGAGAAGAAGCACAAATGTAGTAAGTGCAGCAATTCGTACGGTACAGAATGGGAC
  CTGAAAAGACATGCAGAGGACTGTGGCAAGACCTTCCGGTGCACATGCGGCTGTCCCTACGCCAGTAGA
  ACAGCACTGCAGTCTCACATCTACCGAACTGGGCACGAGATACCTGCAGAACACAGGGACCCACCTAGT
  AAGAAAAGGAAAATGGAAAACTGTGCACAAAACCAGAAGTTATCCAACAAGACCATTGAATCATTGAAC
  AACCAACCAATCCCTAGACCAGACACTCAAGAACTAGAAGCTTCAGAAATAAAGCTAGAACCATCTTTT
  GAAGACTCTTGTGGCTCTAACACTGACAAGCAGACTCTTACAACACCACCGAGATATCCTCAGAAGTTG
  CTTTTACCAAAGCCCAAAGTGGCTTTGGTTAAACTACCCGTGATGCAGTTTTCTGTCATGCCTGTCTTT
  GTGCCTACAGCCGACTCCTCAGCCCAGCCTGTGGTGTTAGGTGTTGATCAGGGCTCTGCCACAGGGGCT
  GTGCACTTAATGCCCTTGTCAGTAGGAACCCTGATCCTCGGCCTAGATTCAGAGGCTTGCTCTCTTAAG
  GAGAGCCTACCTCTTTTCAAAATTGCTAATCCTATTGCTGGTGAGCCAATAAGTACTGGTGTTCAAGTG
  AACTTTGGTAAAAGTCCATCTAATCCTTTACAAGAACTAGGGAACACGTGTCAAAAGAATAGCATTTCT
  TCAATCAACGTGCAGACAGATCTGTCTTATGCCTCACAAAACTTTATACCTTCTGCACAGTGGGCCACT
  GCTGATTCCTCTGTGTCGTCTTGTTCTCAAACTGATTTGTCGTTTGATTCTCAAGTGTCTCTTCCCATT
  AGTGTTCACACTCAGACATTTTTGCCCAGCTCTAAGGTAACTTCATCTATAGCTGCTCAGACTGATGCA
  TTTATGGACACCTGTTTCCAGTCAGGTGGGGTCTCCAGAGAAACTCAAACCAGTGGGATAGAAAGTCCA
  ACGGATGACCATGTACAGATGGACCAAGCTGGAATGTGCGGAGACATTTTTGAGAGTGTTCATTCATCA
  TATAATGTTGCTACAGGTAACATTATAAGCAACAGTTTAGTAGCAGAGACAGTAACTCATAGTTTGTTA
  CCTCAGAATGAGCCTAAGACTTTAAATCAAGATATTGAGAAATCTGCACCAATTATAAATTTCAGTGCA
  CAGAATAGTATGCTTCCTTCACAGAACATGACAGATAATCAGACCCAAACCATAGATTTATTAAGTGAT
  TTGGAAAACATCTTGTCAAGTAATCTGCCTGCTCAGACATTGGATCATCGTAGTCTTTTGTCTGACACA
  AATCCTGGACCTGACACCCAGCTCCCATCTGGCCCAGCCCAGAACCCCGGAATCGATTTTGATATCGAA
  GAGTTCTTTTCGGCCTCAAATATCCAGACTCAAACTGAAGAGAGTGAACTTAGCACCATGACCACCGAG
  CCAGTCTTGGAGTCACTGGACATAGAGACTCAAACGGACTTCTTACTCGCAGATACCTCTGCTCAGTCC
  TATGGGTGTAGGGGAAATTCTAACTTCTTAGGCCTTGAGATGTTTGACACACAGACACAGACAGACTTA
  AACTTTTTCTTAGACAGTAGCCCTCATCTGCCTCTGGGAAGTATTCTGAAACACTCCAGCTTTTCCGTG
  AGTACTGATTCATCTGACACAGAGACCCAAACTGAAGGAGTCTCCACTGCTAAAAATATACCTGCTCTA
  GAAAGCAAAGTTCAGTTGAACAGTACAGAAACACAGACCATGAGTTCTGGGTTTGAAACCCTGGGGAGC
  TTGTTCTTCACCAGCAACGAAACTCAGACAGCAATGGATGACTTTCTTCTGGCTGATCTGGCCTGGAAC
  ACGATGGAGTCTCAGTTCAGCTCTGTAGAAACCCAGACTTCTGCGGAACCACACACAGTCTCCAACTTC
  TAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001300728
• Insert Size: 2004 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001300728.1
• RefSeq Size: 4806 bp
• RefSeq ORF: 2004 bp
• Locus ID: 23300
• UniProt ID: O43313
• Cytogenetics: 16q23.2
• MW: 72.3 kDa
• Gene Summary: Transcription factor. Plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage. Involved in regulating the activity of ATM in the absence of DNA damage. May play a role in stabilizing ATM. Binds to the DYNLL1 promoter and activates its transcription.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.