ASCIZ (ATMIN) (NM_001300728) Human Tagged ORF Clone

CAT#: RC239265

ATMIN (myc-DDK-tagged) - Human ATM interactor (ATMIN), transcript variant 2

Product Images

Circular map for RC239265

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASCIZ
• Synonyms: ASCIZ; ZNF822
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239265 representing NM_001300728
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGCATGCTGAGAAGAAGCACAAATGTAGTAAGTGCAGCAATTCGTACGGTACAGAATGGGACC
  TGAAAAGACATGCAGAGGACTGTGGCAAGACCTTCCGGTGCACATGCGGCTGTCCCTACGCCAGTAGAAC
  AGCACTGCAGTCTCACATCTACCGAACTGGGCACGAGATACCTGCAGAACACAGGGACCCACCTAGTAAG
  AAAAGGAAAATGGAAAACTGTGCACAAAACCAGAAGTTATCCAACAAGACCATTGAATCATTGAACAACC
  AACCAATCCCTAGACCAGACACTCAAGAACTAGAAGCTTCAGAAATAAAGCTAGAACCATCTTTTGAAGA
  CTCTTGTGGCTCTAACACTGACAAGCAGACTCTTACAACACCACCGAGATATCCTCAGAAGTTGCTTTTA
  CCAAAGCCCAAAGTGGCTTTGGTTAAACTACCCGTGATGCAGTTTTCTGTCATGCCTGTCTTTGTGCCTA
  CAGCCGACTCCTCAGCCCAGCCTGTGGTGTTAGGTGTTGATCAGGGCTCTGCCACAGGGGCTGTGCACTT
  AATGCCCTTGTCAGTAGGAACCCTGATCCTCGGCCTAGATTCAGAGGCTTGCTCTCTTAAGGAGAGCCTA
  CCTCTTTTCAAAATTGCTAATCCTATTGCTGGTGAGCCAATAAGTACTGGTGTTCAAGTGAACTTTGGTA
  AAAGTCCATCTAATCCTTTACAAGAACTAGGGAACACGTGTCAAAAGAATAGCATTTCTTCAATCAACGT
  GCAGACAGATCTGTCTTATGCCTCACAAAACTTTATACCTTCTGCACAGTGGGCCACTGCTGATTCCTCT
  GTGTCGTCTTGTTCTCAAACTGATTTGTCGTTTGATTCTCAAGTGTCTCTTCCCATTAGTGTTCACACTC
  AGACATTTTTGCCCAGCTCTAAGGTAACTTCATCTATAGCTGCTCAGACTGATGCATTTATGGACACCTG
  TTTCCAGTCAGGTGGGGTCTCCAGAGAAACTCAAACCAGTGGGATAGAAAGTCCAACGGATGACCATGTA
  CAGATGGACCAAGCTGGAATGTGCGGAGACATTTTTGAGAGTGTTCATTCATCATATAATGTTGCTACAG
  GTAACATTATAAGCAACAGTTTAGTAGCAGAGACAGTAACTCATAGTTTGTTACCTCAGAATGAGCCTAA
  GACTTTAAATCAAGATATTGAGAAATCTGCACCAATTATAAATTTCAGTGCACAGAATAGTATGCTTCCT
  TCACAGAACATGACAGATAATCAGACCCAAACCATAGATTTATTAAGTGATTTGGAAAACATCTTGTCAA
  GTAATCTGCCTGCTCAGACATTGGATCATCGTAGTCTTTTGTCTGACACAAATCCTGGACCTGACACCCA
  GCTCCCATCTGGCCCAGCCCAGAACCCCGGAATCGATTTTGATATCGAAGAGTTCTTTTCGGCCTCAAAT
  ATCCAGACTCAAACTGAAGAGAGTGAACTTAGCACCATGACCACCGAGCCAGTCTTGGAGTCACTGGACA
  TAGAGACTCAAACGGACTTCTTACTCGCAGATACCTCTGCTCAGTCCTATGGGTGTAGGGGAAATTCTAA
  CTTCTTAGGCCTTGAGATGTTTGACACACAGACACAGACAGACTTAAACTTTTTCTTAGACAGTAGCCCT
  CATCTGCCTCTGGGAAGTATTCTGAAACACTCCAGCTTTTCCGTGAGTACTGATTCATCTGACACAGAGA
  CCCAAACTGAAGGAGTCTCCACTGCTAAAAATATACCTGCTCTAGAAAGCAAAGTTCAGTTGAACAGTAC
  AGAAACACAGACCATGAGTTCTGGGTTTGAAACCCTGGGGAGCTTGTTCTTCACCAGCAACGAAACTCAG
  ACAGCAATGGATGACTTTCTTCTGGCTGATCTGGCCTGGAACACGATGGAGTCTCAGTTCAGCTCTGTAG
  AAACCCAGACTTCTGCGGAACCACACACAGTCTCCAACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239265 representing NM_001300728
  Red=Cloning site Green=Tags(s)
  
  MKMHAEKKHKCSKCSNSYGTEWDLKRHAEDCGKTFRCTCGCPYASRTALQSHIYRTGHEIPAEHRDPPSK
  KRKMENCAQNQKLSNKTIESLNNQPIPRPDTQELEASEIKLEPSFEDSCGSNTDKQTLTTPPRYPQKLLL
  PKPKVALVKLPVMQFSVMPVFVPTADSSAQPVVLGVDQGSATGAVHLMPLSVGTLILGLDSEACSLKESL
  PLFKIANPIAGEPISTGVQVNFGKSPSNPLQELGNTCQKNSISSINVQTDLSYASQNFIPSAQWATADSS
  VSSCSQTDLSFDSQVSLPISVHTQTFLPSSKVTSSIAAQTDAFMDTCFQSGGVSRETQTSGIESPTDDHV
  QMDQAGMCGDIFESVHSSYNVATGNIISNSLVAETVTHSLLPQNEPKTLNQDIEKSAPIINFSAQNSMLP
  SQNMTDNQTQTIDLLSDLENILSSNLPAQTLDHRSLLSDTNPGPDTQLPSGPAQNPGIDFDIEEFFSASN
  IQTQTEESELSTMTTEPVLESLDIETQTDFLLADTSAQSYGCRGNSNFLGLEMFDTQTQTDLNFFLDSSP
  HLPLGSILKHSSFSVSTDSSDTETQTEGVSTAKNIPALESKVQLNSTETQTMSSGFETLGSLFFTSNETQ
  TAMDDFLLADLAWNTMESQFSSVETQTSAEPHTVSNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300728
• ORF Size: 2001 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001300728.2
• RefSeq Size: 4806 bp
• RefSeq ORF: 2004 bp
• Locus ID: 23300
• UniProt ID: O43313
• Cytogenetics: 16q23.2
• MW: 72.8 kDa
• Gene Summary: Transcription factor. Plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage. Involved in regulating the activity of ATM in the absence of DNA damage. May play a role in stabilizing ATM. Binds to the DYNLL1 promoter and activates its transcription.[UniProtKB/Swiss-Prot Function]