PDE1A (NM_001258313) Human Untagged Clone

CAT#: SC332656

PDE1A (untagged) - Homo sapiens phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE1A
• Synonyms: CAM-PDE-1A; CAM-PDE 1A; HCAM-1; HCAM1; HSPDE1A
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332656 representing NM_001258313.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGATGACCATGTCACAATCAGGAAGAAACATCTCCAAAGACCCATCTTTAGACTAAGATGCTTGGTG
  AAGCAGCTGGAAAGAGGTGATGTTAACGTCGTCGACTTAAAGAAGAATATTGAATATGCGGCATCTGTG
  CTGGAAGCAGTTTATATCGATGAAACAAGAAGACTTCTGGATACTGAAGATGAGCTCAGTGACATTCAG
  ACTGACTCAGTCCCATCTGAAGTCCGGGACTGGTTGGCTTCTACCTTTACACGGAAAATGGGGATGACA
  AAAAAGAAACCTGAGGAAAAACCAAAATTTCGGAGCATTGTGCATGCTGTTCAAGCTGGAATTTTTGTG
  GAAAGAATGTACCGAAAAACATATCATATGGTTGGTTTGGCATATCCAGCAGCTGTCATCGTAACATTA
  AAGGATGTTGATAAATGGTCTTTCGATGTATTTGCCCTAAATGAAGCAAGTGGAGAGCATAGTCTGAAG
  TTTATGATTTATGAACTGTTTACCAGATATGATCTTATCAACCGTTTCAAGATTCCTGTTTCTTGCCTA
  ATCACCTTTGCAGAAGCTTTAGAAGTTGGTTACAGCAAGTACAAAAATCCATATCACAATTTGATTCAT
  GCAGCTGATGTCACTCAAACTGTGCATTACATAATGCTTCATACAGGTATCATGCACTGGCTCACTGAA
  CTGGAAATTTTAGCAATGGTCTTTGCTGCTGCCATTCATGATTATGAGCATACAGGGACAACAAACAAC
  TTTCACATTCAGACAAGGTCAGATGTTGCCATTTTGTATAATGATCGCTCTGTCCTTGAGAATCACCAC
  GTGAGTGCAGCTTATCGACTTATGCAAGAAGAAGAAATGAATATCTTGATAAATTTATCCAAAGATGAC
  TGGAGGGATCTTCGGAACCTAGTGATTGAAATGGTTTTATCTACAGACATGTCAGGTCACTTCCAGCAA
  ATTAAAAATATAAGAAACAGTTTGCAGCAGCCTGAAGGGATTGACAGAGCCAAAACCATGTCCCTGATT
  CTCCACGCAGCAGACATCAGCCACCCAGCCAAATCCTGGAAGCTGCATTATCGGTGGACCATGGCCCTA
  ATGGAGGAGTTTTTCCTGCAGGGAGATAAAGAAGCTGAATTAGGGCTTCCATTTTCCCCACTTTGTGAT
  CGGAAGTCAACCATGGTGGCCCAGTCACAAATAGGTTTCATCGATTTCATAGTAGAGCCAACATTTTCT
  CTTCTGACAGACTCAACAGAGAAAATTGTTATTCCTCTTATAGAGGAAGCCTCAAAAGCCGAAACTTCT
  TCCTATGTGGCAAGCAGCTCAACCACCATTGTGGGGTTACACATTGCTGATGCACTAAGACGATCAAAT
  ACAAAAGGCTCCATGAGTGATGGGTCCTATTCCCCAGACTACTCCCTTGCAGCAGTGGACCTGAAGAGT
  TTCAAGAACAACCTGGTGGACATCATTCAGCAGAACAAAGAGAGGTGGAAAGAGTTAGCTGCACAAGAA
  GCAAGAACCAGTTCACAGAAGTGTGAGTTTATTCATCAGTAA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001258313
• Insert Size: 1560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258313.1
• RefSeq Size: 2338 bp
• RefSeq ORF: 1560 bp
• Locus ID: 5136
• UniProt ID: P54750
• Cytogenetics: 2q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction
• MW: 59.6 kDa
• Gene Summary: Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]
  Transcript Variant: This variant (4) differs in the 5' and 3' UTRs and uses an alternate start codon, compared to variant 1. The encoded isoform (4) is shorter and has distinct N- and C-termini, compared to isoform 1.