PPP2R5A (NM_001199756) Human Untagged Clone

CAT#: SC331355

PPP2R5A (untagged) - Homo sapiens protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R5A
• Synonyms: B56A; B56alpha; PR61A
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331355 representing NM_001199756.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGATAATGAATGCCACTTCAAATGAACAACAAGAGCTTTTCTGTCAGAAGTTGCAGCAGTGTTGTATA
  CTGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGAGCAAAGAAATTAAAAGAGCAACACTGAATGAA
  CTGGTTGAGTATGTTTCAACTAATCGTGGTGTAATTGTTGAATCAGCGTATTCTGATATAGTAAAAATG
  ATCAGTGCTAACATCTTCCGTACACTTCCTCCAAGTGATAATCCAGATTTTGATCCAGAAGAGGATGAA
  CCCACGCTTGAGGCCTCTTGGCCTCACATACAGTTGGTATATGAATTCTTCTTGAGATTTTTGGAGAGC
  CCTGATTTCCAGCCTAGCATTGCAAAACGATACATTGATCAGAAATTCGTACAACAGCTCCTGGAGCTT
  TTTGATAGTGAAGATCCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCACCGAATTTATGGGAAATTT
  CTTGGATTAAGAGCATTCATCAGAAAACAAATTAACAACATTTTCCTCAGGTTTATATATGAAACAGAA
  CATTTCAATGGTGTTGCTGAACTTCTTGAAATATTAGGAAGTATTATCAATGGCTTTGCATTGCCACTG
  AAAGCAGAACATAAACAATTTCTAATGAAGGTTCTTATTCCTATGCATACTGCAAAAGGATTAGCTTTG
  TTTCATGCTCAGCTAGCATATTGTGTTGTACAGTTCCTGGAGAAAGATACAACACTAACAGAGCCAGTG
  ATCAGAGGACTGCTGAAATTTTGGCCAAAAACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGAAATT
  GAAGAAATCTTAGATGTCATTGAACCAACACAGTTCAAAAAAATTGAAGAGCCACTTTTCAAGCAGATA
  TCCAAGTGTGTATCCAGTTCTCATTTTCAGGTTGCAGAAAGGGCATTGTACTTCTGGAATAACGAATAT
  ATTCTTAGTTTGATTGAGGAGAACATTGATAAAATTCTGCCAATTATGTTTGCCAGTTTGTACAAAATT
  TCCAAAGAACACTGGAATCCGACCATTGTAGCACTGGTATACAATGTGCTGAAAACCCTAATGGAAATG
  AATGGCAAGCTTTTCGATGACCTTACTAGCTCATACAAAGCTGAAAGACAGAGAGAGAAAAAGAAGGAA
  TTGGAACGTGAAGAATTATGGAAAAAATTAGAGGAGCTAAAGCTAAAGAAAGCTCTAGAAAAACAGAAT
  AGTGCTTACAACATGCACAGTATTCTCAGCAATACAAGTGCCGAATAA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199756
• Insert Size: 1290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199756.1
• RefSeq Size: 2667 bp
• RefSeq ORF: 1290 bp
• Locus ID: 5525
• UniProt ID: Q15172
• Cytogenetics: 1q32.3
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Oocyte meiosis, Wnt signaling pathway
• MW: 50.2 kDa
• Gene Summary: The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1.