PPP2R5A (NM_001199756) Human Tagged ORF Clone
CAT#: RG234070
- TrueORF®
PPP2R5A (tGFP-tagged) - Homo sapiens protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 2
ORF Plasmid: DDK
"NM_001199756" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PPP2R5A |
Synonyms | B56A; B56alpha; PR61A |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234070 representing NM_001199756
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAATGAATGCCACTTCAAATGAACAACAAGAGCTTTTCTGTCAGAAGTTGCAGCAGTGTTGTATAC TGTTTGATTTCATGGACTCTGTTTCAGACTTGAAGAGCAAAGAAATTAAAAGAGCAACACTGAATGAACT GGTTGAGTATGTTTCAACTAATCGTGGTGTAATTGTTGAATCAGCGTATTCTGATATAGTAAAAATGATC AGTGCTAACATCTTCCGTACACTTCCTCCAAGTGATAATCCAGATTTTGATCCAGAAGAGGATGAACCCA CGCTTGAGGCCTCTTGGCCTCACATACAGTTGGTATATGAATTCTTCTTGAGATTTTTGGAGAGCCCTGA TTTCCAGCCTAGCATTGCAAAACGATACATTGATCAGAAATTCGTACAACAGCTCCTGGAGCTTTTTGAT AGTGAAGATCCCAGAGAACGTGACTTCCTGAAGACTGTTCTGCACCGAATTTATGGGAAATTTCTTGGAT TAAGAGCATTCATCAGAAAACAAATTAACAACATTTTCCTCAGGTTTATATATGAAACAGAACATTTCAA TGGTGTTGCTGAACTTCTTGAAATATTAGGAAGTATTATCAATGGCTTTGCATTGCCACTGAAAGCAGAA CATAAACAATTTCTAATGAAGGTTCTTATTCCTATGCATACTGCAAAAGGATTAGCTTTGTTTCATGCTC AGCTAGCATATTGTGTTGTACAGTTCCTGGAGAAAGATACAACACTAACAGAGCCAGTGATCAGAGGACT GCTGAAATTTTGGCCAAAAACCTGCAGTCAGAAAGAGGTGATGTTTTTAGGAGAAATTGAAGAAATCTTA GATGTCATTGAACCAACACAGTTCAAAAAAATTGAAGAGCCACTTTTCAAGCAGATATCCAAGTGTGTAT CCAGTTCTCATTTTCAGGTTGCAGAAAGGGCATTGTACTTCTGGAATAACGAATATATTCTTAGTTTGAT TGAGGAGAACATTGATAAAATTCTGCCAATTATGTTTGCCAGTTTGTACAAAATTTCCAAAGAACACTGG AATCCGACCATTGTAGCACTGGTATACAATGTGCTGAAAACCCTAATGGAAATGAATGGCAAGCTTTTCG ATGACCTTACTAGCTCATACAAAGCTGAAAGACAGAGAGAGAAAAAGAAGGAATTGGAACGTGAAGAATT ATGGAAAAAATTAGAGGAGCTAAAGCTAAAGAAAGCTCTAGAAAAACAGAATAGTGCTTACAACATGCAC AGTATTCTCAGCAATACAAGTGCCGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234070 representing NM_001199756
Red=Cloning site Green=Tags(s) MIMNATSNEQQELFCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMI SANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFD SEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAE HKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEIL DVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHW NPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMH SILSNTSAE TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001199756 |
ORF Size | 1287 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001199756.2 |
RefSeq Size | 2667 bp |
RefSeq ORF | 1290 bp |
Locus ID | 5525 |
UniProt ID | Q15172 |
Cytogenetics | 1q32.3 |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | Oocyte meiosis, Wnt signaling pathway |
Gene Summary | The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC234070 | PPP2R5A (Myc-DDK tagged) - Homo sapiens protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 2 |
USD 503.00 |
|
SC331355 | PPP2R5A (untagged) - Homo sapiens protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A), transcript variant 2 |
USD 503.00 |
{0} Product Review(s)
Be the first one to submit a review