AADACL1 (NCEH1) (NM_001146276) Human Untagged Clone

CAT#: SC326995

NCEH1 (untagged)-Human neutral cholesterol ester hydrolase 1 (NCEH1) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCEH1
• Synonyms: AADACL1; NCEH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC326995 representing NM_001146276.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGCAGCTGCCGCGGGCAGAAAGTTGCCGGAGGTCTCCGGGTGGTATCGCCCTTTCCTCTTTGCCAG
  CCCGCTGGCGAGCCGAGCCAGGGCAAGATGAGGTCGTCCTGTGTCCTGCTCACCGCCCTGGTGGCGCTG
  GCCGCCTATTACGTCTACATCCCGCTGCCTGGCTCCGTGTCCGACCCCTGGAAGCTGATGCTGCTGGAC
  GCCACTTTCCGGGGTGCACAGCAAGTGAGTAACCTGATCCACTACCTGGGACTGAGCCATCACCTGCTG
  GCACTGAATTTTATCATTGTTTCTTTTGGCAAAAAAAGCGCGTGGTCTTCTGCCCAAGTGAAGGTGACC
  GACACAGACTTTGATGGTGTGGAAGTCAGAGTGTTTGAAGGCCCTCCGAAGCCCGAAGAGCCACTGAAA
  CGCAGCGTCGTTTATATCCACGGAGGAGGCTGGGCCTTGGCAAGTGCAAGTGCGTCCTGGTCACCTTCA
  GATGAAATCAGGTATTATGATGAGCTGTGTACAGCAATGGCTGAGGAATTGAATGCTGTCATTGTTTCC
  ATTGAATACAGGCTAGTTCCAAAGGTTTATTTTCCTGAGCAAATTCATGATGTTGTACGGGCCACAAAG
  TATTTCCTGAAGCCAGAAGTCTTACAGAAGTATATGGTTGATCCAGGCAGAATTTGCATTTCTGGTGAC
  AGTGCTGGTGGAAATCTGGCTGCTGCCCTTGGACAACAGTTTACTCAAGATGCCAGCCTAAAAAATAAG
  CTCAAACTACAAGCTTTAATTTATCCAGTTCTTCAAGCTTTAGATTTTAACACACCATCTTATCAGCAA
  AATGTGAACACCCCAATCCTGCCCCGCTATGTCATGGTGAAGTATTGGGTGGACTACTTCAAAGGCAAC
  TATGACTTTGTGCAGGCAATGATCGTTAACAATCACACTTCACTTGATGTGGAAGAGGCTGCTGCTGTC
  AGGGCCCGTCTAAACTGGACATCCCTCTTGCCTGCATCCTTCACAAAGAACTACAAGCCTGTTGTACAG
  ACCACAGGCAATGCCAGGATTGTCCAGGAGCTTCCTCAGTTGCTGGATGCCCGCTCCGCCCCACTCATT
  GCAGACCAGGCAGTGCTGCAGCTCCTCCCAAAGACCTACATTCTGACGTGTGAGCATGATGTCCTCAGA
  GACGATGGCATCATGTATGCCAAGCGTTTGGAGAGTGCCGGTGTGGAGGTGACCCTGGATCACTTTGAG
  GATGGCTTTCACGGATGTATGATTTTCACTAGCTGGCCCACCAACTTCTCAGTGGGAATCCGGACTAGG
  AATAGTTACATCAAGTGGCTAGATCAAAACCTGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146276
• Insert Size: 1347 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146276.1
• RefSeq Size: 4318 bp
• RefSeq ORF: 1347 bp
• Locus ID: 57552
• UniProt ID: Q6PIU2
• Cytogenetics: 3q26.31
• Protein Families: Transmembrane
• MW: 49.9 kDa
• Gene Summary: Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by available conservation data.