AADACL1 (NCEH1) (NM_001146276) Human Tagged ORF Clone

CAT#: RG228360

NCEH1 (tGFP-tagged) - Human neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 1

Product Images

Circular map for RG228360

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AADACL1
• Synonyms: AADACL1; NCEH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228360 representing NM_001146276
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCTGCCGCGGGCAGAAAGTTGCCGGAGGTCTCCGGGTGGTATCGCCCTTTCCTCTTTGCCAGC
  CCGCTGGCGAGCCGAGCCAGGGCAAGATGAGGTCGTCCTGTGTCCTGCTCACCGCCCTGGTGGCGCTGGC
  CGCCTATTACGTCTACATCCCGCTGCCTGGCTCCGTGTCCGACCCCTGGAAGCTGATGCTGCTGGACGCC
  ACTTTCCGGGGTGCACAGCAAGTGAGTAACCTGATCCACTACCTGGGACTGAGCCATCACCTGCTGGCAC
  TGAATTTTATCATTGTTTCTTTTGGCAAAAAAAGCGCGTGGTCTTCTGCCCAAGTGAAGGTGACCGACAC
  AGACTTTGATGGTGTGGAAGTCAGAGTGTTTGAAGGCCCTCCGAAGCCCGAAGAGCCACTGAAACGCAGC
  GTCGTTTATATCCACGGAGGAGGCTGGGCCTTGGCAAGTGCAAGTGCGTCCTGGTCACCTTCAGATGAAA
  TCAGGTATTATGATGAGCTGTGTACAGCAATGGCTGAGGAATTGAATGCTGTCATTGTTTCCATTGAATA
  CAGGCTAGTTCCAAAGGTTTATTTTCCTGAGCAAATTCATGATGTTGTACGGGCCACAAAGTATTTCCTG
  AAGCCAGAAGTCTTACAGAAGTATATGGTTGATCCAGGCAGAATTTGCATTTCTGGTGACAGTGCTGGTG
  GAAATCTGGCTGCTGCCCTTGGACAACAGTTTACTCAAGATGCCAGCCTAAAAAATAAGCTCAAACTACA
  AGCTTTAATTTATCCAGTTCTTCAAGCTTTAGATTTTAACACACCATCTTATCAGCAAAATGTGAACACC
  CCAATCCTGCCCCGCTATGTCATGGTGAAGTATTGGGTGGACTACTTCAAAGGCAACTATGACTTTGTGC
  AGGCAATGATCGTTAACAATCACACTTCACTTGATGTGGAAGAGGCTGCTGCTGTCAGGGCCCGTCTAAA
  CTGGACATCCCTCTTGCCTGCATCCTTCACAAAGAACTACAAGCCTGTTGTACAGACCACAGGCAATGCC
  AGGATTGTCCAGGAGCTTCCTCAGTTGCTGGATGCCCGCTCCGCCCCACTCATTGCAGACCAGGCAGTGC
  TGCAGCTCCTCCCAAAGACCTACATTCTGACGTGTGAGCATGATGTCCTCAGAGACGATGGCATCATGTA
  TGCCAAGCGTTTGGAGAGTGCCGGTGTGGAGGTGACCCTGGATCACTTTGAGGATGGCTTTCACGGATGT
  ATGATTTTCACTAGCTGGCCCACCAACTTCTCAGTGGGAATCCGGACTAGGAATAGTTACATCAAGTGGC
  TAGATCAAAACCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228360 representing NM_001146276
  Red=Cloning site Green=Tags(s)
  
  MSSCRGQKVAGGLRVVSPFPLCQPAGEPSQGKMRSSCVLLTALVALAAYYVYIPLPGSVSDPWKLMLLDA
  TFRGAQQVSNLIHYLGLSHHLLALNFIIVSFGKKSAWSSAQVKVTDTDFDGVEVRVFEGPPKPEEPLKRS
  VVYIHGGGWALASASASWSPSDEIRYYDELCTAMAEELNAVIVSIEYRLVPKVYFPEQIHDVVRATKYFL
  KPEVLQKYMVDPGRICISGDSAGGNLAAALGQQFTQDASLKNKLKLQALIYPVLQALDFNTPSYQQNVNT
  PILPRYVMVKYWVDYFKGNYDFVQAMIVNNHTSLDVEEAAAVRARLNWTSLLPASFTKNYKPVVQTTGNA
  RIVQELPQLLDARSAPLIADQAVLQLLPKTYILTCEHDVLRDDGIMYAKRLESAGVEVTLDHFEDGFHGC
  MIFTSWPTNFSVGIRTRNSYIKWLDQNL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146276
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146276.1, NP_001139748.1
• RefSeq Size: 4318 bp
• RefSeq ORF: 1251 bp
• Locus ID: 57552
• UniProt ID: Q6PIU2
• Cytogenetics: 3q26.31
• Protein Families: Transmembrane
• Gene Summary: Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.[UniProtKB/Swiss-Prot Function]