RASGRP3 (NM_001139488) Human Untagged Clone

CAT#: SC325277

RASGRP3 (untagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASGRP3
• Synonyms: GRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC325277 representing NM_001139488.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGATCAAGTGGCCTTGGGAAAGCAGCAACATTAGATGAACTGCTGTGCACTTGCATTGAGATGTTT
  GATGACAATGGAGAGCTGGATAATAGTTATTTGCCAAGAATAGTTCTACTGATGCACCGATGGTATTTA
  TCTTCCACTGAATTGGCAGAAAAACTTCTCTGCATGTATCGAAATGCCACTGGAGAAAGCTGCAATGAA
  TTTCGATTAAAGATCTGCTACTTCATGAGGTACTGGATTCTGAAGTTTCCTGCAGAGTTTAATTTGGAT
  CTTGGTTTGATTCGTATGACTGAGGAATTTCGGGAAGTAGCTAGTCAACTAGGATATGAAAAACACGTC
  AGCCTCATCGACATATCCAGCATTCCTTCCTATGACTGGATGAGAAGAGTCACACAGAGGAAAAAAGTA
  TCCAAGAAGGGAAAAGCCTGTCTGCTGTTTGACCATCTGGAGCCCATTGAATTGGCTGAGCACCTCACT
  TTTCTGGAGCATAAATCTTTTAGAAGGATCTCATTCACTGATTACCAAAGCTATGTCATCCATGGCTGC
  CTGGAGAATAATCCAACCTTGGAAAGATCGATTGCTTTATTTAATGGAATCTCTAAGTGGGTCCAGTTG
  ATGGTTCTTAGCAAACCAACCCCCCAGCAAAGGGCAGAAGTCATCACAAAGTTTATCAATGTTGCAAAG
  AAGCTCCTTCAGCTCAAAAATTTTAACACCCTGATGGCAGTGGTGGGAGGCCTCAGTCATAGTTCCATT
  TCACGCCTCAAAGAGACCCATTCTCATCTTTCTTCAGAAGTTACAAAGAACTGGAATGAAATGACAGAG
  TTGGTCTCCTCCAACGGCAATTACTGCAATTACCGCAAGGCCTTTGCCGACTGCGATGGCTTCAAAATC
  CCCATCCTTGGAGTACACTTGAAAGACTTGATAGCTGTCCATGTCATTTTCCCAGACTGGACAGAGGAG
  AACAAAGTGAACATTGTGAAAATGCACCAGCTCTCCGTTACCCTGAGTGAACTAGTCTCCCTGCAGAAT
  GCCTCTCACCACTTAGAACCCAACATGGATTTGATCAACCTGCTCACGCTTTCCCTGGACCTCTATCAC
  ACTGAAGATGATATTTACAAACTGTCACTGGTGCTGGAGCCTAGAAATTCTAAATCGCAGCCTACCTCC
  CCTACGACGCCCAACAAGCCTGTGGTACCCCTGGAGTGGGCATTAGGGGTGATGCCAAAGCCAGACCCC
  ACGGTCATCAACAAGCACATAAGGAAATTAGTGGAGTCTGTATTTAGAAACTATGATCACGACCATGAT
  GGGTACATTTCCCAAGAGGACTTTGAAAGTATAGCTGCCAATTTTCCCTTCTTGGATTCCTTCTGTGTT
  CTGGACAAAGATCAGGATGGCCTAATTAGTAAAGATGAAATGATGGCTTACTTCCTGAGAGCTAAATCC
  CAACTACACTGTAAAATGGGACCAGGATTTATCCATAATTTTCAGGAGATGACCTATCTCAAGCCAACC
  TTCTGCGAACACTGTGCGGGATTTCTCTGGGGCATAATCAAGCAAGGATACAAATGCAAAGACTGTGGA
  GCCAATTGTCACAAACAGTGCAAAGACCTCCTGGTTCTGGCCTGCAGGAGATTTGCCCGGGCGCCCTCC
  TTGAGCAGTGGTCATGGGTCACTGCCTGGAAGCCCCTCGCTGCCCCCAGCGCAGGATGAGGTGTTTGAG
  TTCCCTGGAGTCACTGCTGGACACAGGGATTTAGACAGCAGAGCCATCACACTGGTTACAGGCTCTTCT
  CGCAAGATCTCTGTGAGGCTACAGAGGGCCACCACCAGCCAGGCCACCCAGACTGAACCTGTCTGGTCA
  GAGGCTGGCTGGGGGGACTCGGGGTCCCACACCTTCCCTAAAATGAAATCCAAGTTCCATGACAAAGCA
  GCAAAGGACAAAGGCTTTGCCAAATGGGAAAATGAGAAGCCCAGGGTGCATGCTGGTGTGGATGTTGTA
  GACCGGGGCACGGAGTTTGAACTTGACCAGGATGAAGGAGAAGAGACCAGACAGGATGGTGAGGATGGC
  TGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001139488
• Insert Size: 2073 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001139488.1
• RefSeq Size: 4798 bp
• RefSeq ORF: 2073 bp
• Locus ID: 25780
• UniProt ID: Q8IV61
• Cytogenetics: 2p22.3
• Protein Pathways: B cell receptor signaling pathway, MAPK signaling pathway
• MW: 78.3 kDa
• Gene Summary: The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1, 2, and 4-6 all encode the same isoform (1).