Thimet Oligopeptidase (THOP1) (NM_003249) Human Untagged Clone

CAT#: SC324358

THOP1 (untagged)-Human thimet oligopeptidase 1 (THOP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Thimet Oligopeptidase
• Synonyms: EP24.15; MEPD_HUMAN; MP78; TOP
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003249.3 GCCCCGGGAGCGCGGGCGGCGGGCCCCTTGGTCCTCAGGCGGCCGTGGCGGCGGTGGCGG
  CGGTTGGGCCGAGGCAGGCGGCCTCAGTGGCCGAGGTGGCTGGACGCGTAGCAGGTGGAA
  GGAGGGAGGGAGCCGCAGGCGCAGACCCACCCGCCATGAAGCCCCCCGCAGCCTGTGCAG
  GAGACATGGCGGACGCAGCATCTCCGTGCTCTGTGGTAAACGACCTGCGGTGGGACCTGA
  GTGCCCAGCAGATAGAGGAGCGCACCAGGGAGCTCATCGAGCAGACCAAGCGCGTGTATG
  ACCAGGTTGGCACCCAGGAGTTTGAGGACGTGTCCTACGAGAGCACGCTCAAGGCGCTGG
  CCGATGTGGAGGTCACCTACACAGTTCAGAGGAATATCCTTGACTTCCCCCAGCATGTTT
  CCCCCTCCAAGGACATCCGGACAGCCAGCACAGAGGCCGACAAGAAGCTCTCTGAGTTCG
  ACGTGGAGATGAGCATGAGGGAGGACGTGTACCAGAGGATCGTGTGGCTCCAGGAGAAAG
  TTCAGAAGGACTCACTGAGGCCCGAGGCTGCGCGGTACCTGGAGCGGCTAATCAAGCTGG
  GCCGGAGAAATGGGCTTCACCTCCCCAGAGAGACTCAGGAAAACATCAAACGCATCAAGA
  AGAAGCTGAGCCTTCTGTGCATCGACTTCAACAAGAACCTGAACGAGGACACGACCTTCC
  TGCCCTTCACGCTCCAGGAGCTAGGAGGGCTCCCCGAGGACTTTCTGAACTCCCTGGAGA
  AGATGGAGGACGGCAAGTTGAAGGTCACCCTCAAGTACCCCCATTACTTCCCCCTCCTGA
  AGAAATGCCACGTGCCTGAGACCAGGAGGAAAGTGGAGGAGGCCTTCAACTGCCGGTGCA
  AGGAGGAGAACTGCGCTATCCTCAAGGAGCTGGTGACGCTGCGGGCCCAGAAGTCCCGCC
  TGCTGGGGTTCCACACGCACGCCGACTATGTCCTGGAGATGAACATGGCCAAGACCAGCC
  AGACCGTGGCCACCTTCCTAGATGAGCTGGCGCAGAAGCTGAAGCCCCTGGGGGAGCAGG
  AGCGTGCGGTGATTCTGGAGCTGAAGCGTGCGGAGTGCGAGCGCCGGGGCCTGCCCTTCG
  ACGGCCGCATCCGTGCCTGGGACATGCGCTACTACATGAACCAGGTGGAGGAGACGCGCT
  ACTGCGTGGACCAGAACCTGCTCAAGGAGTACTTCCCCGTGCAGGTGGTCACGCACGGGC
  TGCTGGGCATCTACCAGGAGCTCCTGGGGCTGGCCTTCCACCACGAGGAGGGCGCCAGTG
  CCTGGCATGAGGACGTGCGGCTCTACACCGCGAGGGACGCGGCCTCGGGGGAGGTGGTCG
  GCAAGTTCTACCTGGACCTGTACCCGCGGGAAGGAAAGTACGGGCACGCGGCCTGCTTTG
  GCCTGCAGCCCGGCTGCCTGCGGCAGGATGGGAGCCGCCAGATCGCCATCGCGGCCATGG
  TGGCCAACTTCACCAAGCCCACAGCCGACGCGCCCTCGCTGCTGCAGCATGACGAGGTGG
  AGACCTACTTCCATGAGTTTGGCCACGTGATGCACCAGCTCTGCTCCCAGGCGGAGTTCG
  CCATGTTCAGCGGGACCCACGTGGAGCGGGACTTTGTGGAGGCGCCGTCGCAGATGCTGG
  AGAACTGGGTGTGGGAGCAGGAGCCGCTGCTGCGGATGTCGCGGCACTACCGCACAGGCA
  GCGCCGTGCCCCGGGAGCTCCTGGAGAAGCTCATTGAGTCCCGGCAGGCCAACACAGGCC
  TCTTCAACCTGCGCCAGATCGTCCTCGCCAAGGTGGACCAGGCCCTGCACACGCAGACGG
  ACGCAGACCCCGCCGAGGAGTATGCGCGGCTCTGCCAGGAGATCCTCGGGGTCCCGGCCA
  CGCCAGGAACCAACATGCCTGCAACCTTCGGCCATCTGGCAGGTGGCTACGACGCCCAGT
  ACTACGGGTACCTGTGGAGCGAGGTGTATTCCATGGACATGTTCCACACGCGCTTCAAGC
  AGGAGGGTGTCCTGAACAGCAAGGTTGGCATGGATTACAGAAGCTGCATCCTGAGACCCG
  GCGGTTCCGAGGATGCCAGCGCCATGCTGAGGCGCTTCCTGGGCCGTGACCCCAAGCAGG
  ACGCCTTCCTCCTGAGCAAGGGGCTGCAGGTCGGGGGCTGCGAGCCCGAGCCGCAGGTCT
  GCTGAGGCCTGGCACTGCGACTGCCCAGTCTGGCCTGCGCTCCCGCCGCCCTGGTGCCTT
  AGCCCCCGGCACAGGATGGGGCAGGCTCTGGCACAGTGCCTGGGACTGGCAGGGTGGCTG
  AGCGGCTGTCTTGCCTCTTGTCATTGTCTGTCCCCACCCGGTCGTGGCCCACCCGGCTAG
  AGACGGCGTCCTCAAGGCATCTGGAGGGCTTTCGTGGCTGCCAGGGCCTGGTCTTTGTTG
  CACTAACACGTCTCCTCTCTGGGAAACGTCCCTTGTCAGGAGACGGCTCTTCTTTGAAAT
  GAGGTCATTAAAAGGAAACAGAAAAAGAGAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003249
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003249.3, NP_003240.1
• RefSeq Size: 2568 bp
• RefSeq ORF: 2070 bp
• Locus ID: 7064
• UniProt ID: P52888
• Cytogenetics: 19p13.3
• Domains: Peptidase_M3
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Renin-angiotensin system
• Gene Summary: The protein encoded by this gene is a kininase that uses zinc as a cofactor. The encoded oligopeptidase cleaves cytosolic peptides, making them unavailable for display on antigen-presenting cells. This protein also cleaves neuropeptides under 20 aa in length and can degrade beta-amyloid precursor protein to amyloidogenic peptides. [provided by RefSeq, Nov 2015]