Thimet Oligopeptidase (THOP1) (NM_003249) Human Tagged ORF Clone

CAT#: RC201738

THOP1 (Myc-DDK-tagged)-Human thimet oligopeptidase 1 (THOP1)

Product Images

Circular map for RC201738

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY THOP1 (Cat# RC201738, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-THOP1 (Cat# TA501409). Positive lysates LY401123 (100ug) and LC401123 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401123) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201738 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified THOP1 protein (Cat# TP301738). The protein was produced from HEK293T cells transfected with THOP1 cDNA clone (Cat# RC201738) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Thimet Oligopeptidase
• Synonyms: EP24.15; MEPD_HUMAN; MP78; TOP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201738 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCCCCCGCAGCCTGTGCAGGAGACATGGCGGACGCAGCATCTCCGTGCTCTGTGGTAAACGACC
  TGCGGTGGGACCTGAGTGCCCAGCAGATAGAGGAGCGCACCAGGGAGCTCATCGAGCAGACCAAGCGCGT
  GTATGACCAGGTTGGCACCCAGGAGTTTGAGGACGTGTCCTACGAGAGCACGCTCAAGGCGCTGGCCGAT
  GTGGAGGTCACCTACACAGTTCAGAGGAATATCCTTGACTTCCCCCAGCATGTTTCCCCCTCCAAGGACA
  TCCGGACAGCCAGCACAGAGGCCGACAAGAAGCTCTCTGAGTTCGACGTGGAGATGAGCATGAGGGAGGA
  CGTGTACCAGAGGATCGTGTGGCTCCAGGAGAAAGTTCAGAAGGACTCACTGAGGCCCGAGGCTGCGCGG
  TACCTGGAGCGGCTAATCAAGCTGGGCCGGAGAAATGGGCTTCACCTCCCCAGAGAGACTCAGGAAAACA
  TCAAACGCATCAAGAAGAAGCTGAGCCTTCTGTGCATCGACTTCAACAAGAACCTGAACGAGGACACGAC
  CTTCCTGCCCTTCACGCTCCAGGAGCTAGGAGGGCTCCCCGAGGACTTTCTGAACTCCCTGGAGAAGATG
  GAGGACGGCAAGTTGAAGGTCACCCTCAAGTACCCCCATTACTTCCCCCTCCTGAAGAAATGCCACGTGC
  CTGAGACCAGGAGGAAAGTGGAGGAGGCCTTCAACTGCCGGTGCAAGGAGGAGAACTGCGCTATCCTCAA
  GGAGCTGGTGACGCTGCGGGCCCAGAAGTCCCGCCTGCTGGGGTTCCACACGCACGCCGACTATGTCCTG
  GAGATGAACATGGCCAAGACCAGCCAGACCGTGGCCACCTTCCTAGATGAGCTGGCGCAGAAGCTGAAGC
  CCCTGGGGGAGCAGGAGCGTGCGGTGATTCTGGAGCTGAAGCGTGCGGAGTGCGAGCGCCGGGGCCTGCC
  CTTCGACGGCCGCATCCGTGCCTGGGACATGCGCTACTACATGAACCAGGTGGAGGAGACGCGCTACTGC
  GTGGACCAGAACCTGCTCAAGGAGTACTTCCCCGTGCAGGTGGTCACGCACGGGCTGCTGGGCATCTACC
  AGGAGCTCCTGGGGCTGGCCTTCCACCACGAGGAGGGCGCCAGTGCCTGGCATGAGGACGTGCGGCTCTA
  CACCGCGAGGGACGCGGCCTCGGGGGAGGTGGTCGGCAAGTTCTACCTGGACCTGTACCCGCGGGAAGGA
  AAGTACGGGCACGCGGCCTGCTTTGGCCTGCAGCCCGGCTGCCTGCGGCAGGATGGGAGCCGCCAGATCG
  CCATCGCGGCCATGGTGGCCAACTTCACCAAGCCCACAGCCGACGCGCCCTCGCTGCTGCAGCATGACGA
  GGTGGAGACCTACTTCCATGAGTTTGGCCACGTGATGCACCAGCTCTGCTCCCAGGCGGAGTTCGCCATG
  TTCAGCGGGACCCACGTGGAGCGGGACTTTGTGGAGGCGCCGTCGCAGATGCTGGAGAACTGGGTGTGGG
  AGCAGGAGCCGCTGCTGCGGATGTCGCGGCACTACCGCACAGGCAGCGCCGTGCCCCGGGAGCTCCTGGA
  GAAGCTCATTGAGTCCCGGCAGGCCAACACAGGCCTCTTCAACCTGCGCCAGATCGTCCTCGCCAAGGTG
  GACCAGGCCCTGCACACGCAGACGGACGCAGACCCCGCCGAGGAGTATGCGCGGCTCTGCCAGGAGATCC
  TCGGGGTCCCGGCCACGCCAGGAACCAACATGCCTGCAACCTTCGGCCATCTGGCAGGTGGCTACGACGC
  CCAGTACTACGGGTACCTGTGGAGCGAGGTGTATTCCATGGACATGTTCCACACGCGCTTCAAGCAGGAG
  GGTGTCCTGAACAGCAAGGTTGGCATGGATTACAGAAGCTGCATCCTGAGACCCGGCGGTTCCGAGGATG
  CCAGCGCCATGCTGAGGCGCTTCCTGGGCCGTGACCCCAAGCAGGACGCCTTCCTCCTGAGCAAGGGGCT
  GCAGGTCGGGGGCTGCGAGCCCGAGCCGCAGGTCTGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201738 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKPPAACAGDMADAASPCSVVNDLRWDLSAQQIEERTRELIEQTKRVYDQVGTQEFEDVSYESTLKALAD
  VEVTYTVQRNILDFPQHVSPSKDIRTASTEADKKLSEFDVEMSMREDVYQRIVWLQEKVQKDSLRPEAAR
  YLERLIKLGRRNGLHLPRETQENIKRIKKKLSLLCIDFNKNLNEDTTFLPFTLQELGGLPEDFLNSLEKM
  EDGKLKVTLKYPHYFPLLKKCHVPETRRKVEEAFNCRCKEENCAILKELVTLRAQKSRLLGFHTHADYVL
  EMNMAKTSQTVATFLDELAQKLKPLGEQERAVILELKRAECERRGLPFDGRIRAWDMRYYMNQVEETRYC
  VDQNLLKEYFPVQVVTHGLLGIYQELLGLAFHHEEGASAWHEDVRLYTARDAASGEVVGKFYLDLYPREG
  KYGHAACFGLQPGCLRQDGSRQIAIAAMVANFTKPTADAPSLLQHDEVETYFHEFGHVMHQLCSQAEFAM
  FSGTHVERDFVEAPSQMLENWVWEQEPLLRMSRHYRTGSAVPRELLEKLIESRQANTGLFNLRQIVLAKV
  DQALHTQTDADPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQE
  GVLNSKVGMDYRSCILRPGGSEDASAMLRRFLGRDPKQDAFLLSKGLQVGGCEPEPQVC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003249
• ORF Size: 2067 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003249.5
• RefSeq Size: 2568 bp
• RefSeq ORF: 2070 bp
• Locus ID: 7064
• UniProt ID: P52888
• Cytogenetics: 19p13.3
• Domains: Peptidase_M3
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Renin-angiotensin system
• MW: 78.8 kDa
• Gene Summary: The protein encoded by this gene is a kininase that uses zinc as a cofactor. The encoded oligopeptidase cleaves cytosolic peptides, making them unavailable for display on antigen-presenting cells. This protein also cleaves neuropeptides under 20 aa in length and can degrade beta-amyloid precursor protein to amyloidogenic peptides. [provided by RefSeq, Nov 2015]