Thimet Oligopeptidase (THOP1) (NM_003249) Human Untagged Clone

CAT#: SC118078

THOP1 (untagged)-Human thimet oligopeptidase 1 (THOP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Thimet Oligopeptidase
• Synonyms: EP24.15; MEPD_HUMAN; MP78; TOP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118078 sequence for NM_003249 edited (data generated by NextGen Sequencing)
  ATGAAGCCCCCCGCAGCCTGTGCAGGAGACATGGCGGACGCAGCATCTCCGTGCTCTGTG
  GTAAACGACCTGCGGTGGGACCTGAGTGCCCAGCAGATAGAGGAGCGCACCAGGGAGCTC
  ATCGAGCAGACCAAGCGCGTGTATGACCAGGTTGGCACCCAGGAGTTTGAGGACGTGTCC
  TACGAGAGCACGCTCAAGGCGCTGGCCGATGTGGAGGTCACCTACACAGTTCAGAGGAAT
  ATCCTTGACTTCCCCCAGCATGTTTCCCCCTCCAAGGACATCCGGACAGCCAGCACAGAG
  GCCGACAAGAAGCTCTCTGAGTTCGACGTGGAGATGAGCATGAGGGAGGACGTGTACCAG
  AGGATCGTGTGGCTCCAGGAGAAAGTTCAGAAGGACTCACTGAGGCCCGAGGCTGCGCGG
  TACCTGGAGCGGCTAATCAAGCTGGGCCGGAGAAATGGGCTTCACCTCCCCAGAGAGACT
  CAGGAAAACATCAAACGCATCAAGAAGAAGCTGAGCCTTCTGTGCATCGACTTCAACAAG
  AACCTGAACGAGGACACGACCTTCCTGCCCTTCACGCTCCAGGAGCTAGGAGGGCTCCCC
  GAGGACTTTCTGAACTCCCTGGAGAAGATGGAGGACGGCAAGTTGAAGGTCACCCTCAAG
  TACCCCCATTACTTCCCCCTCCTGAAGAAATGCCACGTGCCTGAGACCAGGAGGAAAGTG
  GAGGAGGCCTTCAACTGCCGGTGCAAGGAGGAGAACTGCGCTATCCTCAAGGAGCTGGTG
  ACGCTGCGGGCCCAGAAGTCCCGCCTGCTGGGGTTCCACACGCACGCCGACTATGTCCTG
  GAGATGAACATGGCCAAGACCAGCCAGACCGTGGCCACCTTCCTAGATGAGCTGGCGCAG
  AAGCTGAAGCCCCTGGGGGAGCAGGAGCGTGCGGTGATTCTGGAGCTGAAGCGTGCGGAG
  TGCGAGCGCCGGGGCCTGCCCTTCGACGGCCGCATCCGTGCCTGGGACATGCGCTACTAC
  ATGAACCAGGTGGAGGAGACGCGCTACTGCGTGGACCAGAACCTGCTCAAGGAGTACTTC
  CCCGTGCAGGTGGTCACGCACGGGCTGCTGGGCATCTACCAGGAGCTCCTGGGGCTGGCC
  TTCCACCACGAGGAGGGCGCCAGTGCCTGGCATGAGGACGTGCGGCTCTACACCGCGAGG
  GACGCGGCCTCGGGGGAGGTGGTCGGCAAGTTCTACCTGGACCTGTACCCGCGGGAAGGA
  AAGTACGGGCACGCGGCCTGCTTTGGCCTGCAGCCCGGCTGCCTGCGGCAGGATGGGAGC
  CGCCAGATCGCCATCGCGGCCATGGTGGCCAACTTCACCAAGCCCACAGCCGACGCGCCC
  TCGCTGCTGCAGCATGACGAGGTGGAGACCTACTTCCATGAGTTTGGCCACGTGATGCAC
  CAGCTCTGCTCCCAGGCGGAGTTCGCCATGTTCAGCGGGACCCACGTGGAGCGGGACTTT
  GTGGAGGCGCCGTCGCAGATGCTGGAGAACTGGGTGTGGGAGCAGGAGCCGCTGCTGCGG
  ATGTCGCGGCACTACCGCACAGGCAGCGCCGTGCCCCGGGAGCTCCTGGAGAAGCTCATT
  GAGTCCCGGCAGGCCAACACAGGCCTCTTCAACCTGCGCCAGATCGTCCTCGCCAAGGTG
  GACCAGGCCCTGCACACGCAGACGGACGCAGACCCCGCCGAGGAGTATGCGCGGCTCTGC
  CAGGAGATCCTCGGGGTCCCGGCCACGCCAGGAACCAACATGCCTGCAACCTTCGGCCAT
  CTGGCAGGTGGCTACGACGCCCAGTACTACGGGTACCTGTGGAGCGAGGTGTATTCCATG
  GACATGTTCCACACGCGCTTCAAGCAGGAGGGTGTCCTGAACAGCAAGGTTGGCATGGAT
  TACAGAAGCTGCATCCTGAGACCCGGCGGTTCCGAGGATGCCAGCGCCATGCTGAGGCGC
  TTCCTGGGCCGTGACCCCAAGCAGGACGCCTTCCTCCTGAGCAAGGGGCTGCAGGTCGGG
  GGCTGCGAGCCCGAGCCGCAGGTCTGCTGA
  
  Clone variation with respect to NM_003249.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003249 unedited
  ATTTTGTAATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGGGGCGGCGGGCCC
  CTTGGTCCTCAGGCGGCCGTGGCGGCGGTGGCGGCGGTTGGGCCGAGGCAGGCGGCCTCA
  GTGGCCGAGGTGGCTGGACGCGTAGCAGGTGGAAGGAGGGAGGGAGCCGCAGGCGCAGAC
  CCACCCGCCATGAAGCCCCCCGCAGCCTGTGCAGGAGACATGGCGGACGCAGCATCTCCG
  TGCTCTGTGGTAAACGACCTGCGGTGGGACCTGAGTGCCCAGCAGATAGAGGAGCGCACC
  AGGGAGCTCATCGAGCAGACCAAGCGCGTGTATGACCAGGTTGGCACCCAGGAGTTTGAG
  GACGTGTCCTACGAGAGCACGCTCAAGGCGCTGGCCGATGTGGAGGTCACCTACACAGTT
  CAGAGGAATATCCTTGACTTCCCCCAGCATGTTTCCCCCTCCAAGGACATCCGGACAGCC
  AGCACAGAGGCCGACAAGAAGCTCTCTGAGTTCGACGTGGAGATGAGCATGAGGGAGGAC
  GTGTACCAGAGGATCGTGTGGCTCCAGGAGAAAGTTCAGAAGGACTCACTGAGGCCCGAG
  GCTGCGCGGTACCTGGAGCGGCTAATCAAGCTGGGCCGGAGAAATGGGCTTCACCTCCCC
  AGAGAGACTCAGGANAACATCAAACGCATCAAGAAGAAGCTGAGCCTTCTGTGCATCGAC
  TTCAACAAGAACCTGAACGAGGACACGACCTTCCTGCCCTTCACGCTCCAGGAGCTAGGA
  GGGCTCCCCGAGGACTTTCTGAAACTCCTGGAGAAGATGGNAGACNGGCAGNTGAAGGTC
  ACCCNTTCAGTACCCCATTACTTTCCCCTNNCTGAGAAATGCCACGTGCCTGAGACCAGN
  NNAGAAGTGGNAGAGGCCCTTCACTGNCCNGTGCAGGGAGAGACTGCGCTTNCTCAGGAG
  CTGTGACGCTCNGNCCAAAGTCCGCTGCTGGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003249 unedited
  CGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCTGTTTCCTTTTAATGACC
  TCATTTCAAAAAAAAGCCGTCTCCTGACAAGGGACGTTTCCCAGAGAGGAGACGTGTTAG
  TGCAACAAAGACCAGGCCCTGGCAGCCACGAAAGCCCTCCAGATGCCTTGAGGACGCCGT
  CTCTAGCCGGGTGGGCCACGACCGGGTGGGGACAGACAATGACAAGAGGCAAGACAGCCG
  CTCAGCCACCCTGCCAGTCCCAGGCACTGTGCCAGAGCCTGCCCCATCCTGTGCCGGGGG
  CTAAGGCACCAGGGCGGCGGGAGCGCAGGCCAGACTGGGCAGTCGCAGTGCCAGGCCTCA
  GCAGACCTGCGGCTCGGGCTCGCAGCCCCCGACCTGCAGCCCCTTGCTCAGGAGGAAGGC
  GTCCTGCTTGGGGTCACCGGCCCAGGAAGCGCCTCAGCATGGCGCTGGCATCCTCGGAAC
  CGCCGGGTCTCAGGATGCAGCTTCTGTAATCCATGCCAACCTTGCTGTTCAGGACACCCT
  CCTGCTTGAAGCGCGTGTGGAACATGTNCATGGAATACACCTCGCTCCACAGGTACCCGT
  AGCACTGCGCGTCGTACCCACCTGCCAGATTGGCCGAAGCTGCAGGCATGCTGGTTCCTG
  GCGTGCCCGGGACCCNGAGGATCTCCTGCCAGAGCCGCGCATACTCCTCGGTGGGGTCTG
  CGCCCGTTTGCGTGTGCAAGGCCTGATCCACCTGGCCGAGGACAACCTGGCGCACGTTGA
  AAAAGCCTGTTGTTGCCCGCCCGGACTCATTGAGCTTCTCCAGAACCTACCGGGGCAAGG
  TCCTGCCTGGGCGGTAATGCCGCAACTTCCGCATCAAGGGTTCCTTGGTCCCAACCCCAG
  TTTCTCACAATTGTGGGAAGCGGCCTCACACAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003249
• Insert Size: 2680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003249.3, NP_003240.1
• RefSeq Size: 2568 bp
• RefSeq ORF: 2070 bp
• Locus ID: 7064
• UniProt ID: P52888
• Cytogenetics: 19p13.3
• Domains: Peptidase_M3
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Renin-angiotensin system
• Gene Summary: The protein encoded by this gene is a kininase that uses zinc as a cofactor. The encoded oligopeptidase cleaves cytosolic peptides, making them unavailable for display on antigen-presenting cells. This protein also cleaves neuropeptides under 20 aa in length and can degrade beta-amyloid precursor protein to amyloidogenic peptides. [provided by RefSeq, Nov 2015]