PIAS3 (NM_006099) Human Untagged Clone

CAT#: SC324304

PIAS3 (untagged)-Human protein inhibitor of activated STAT, 3 (PIAS3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIAS3
• Synonyms: ZMIZ5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006099.3 CTGGGCCTGAGCTCCGGCTCCGGCTGGGGCGCCTGCGATGTCTCAAGATGGCGGAGCTGG
  GCGAATTAAAGCACATGGTGATGAGTTTCCGGGTGTCTGAGCTCCAGGTGCTTCTTGGCT
  TTGCTGGCCGGAACAAGAGTGGACGGAAGCACGAGCTCCTGGCCAAGGCTCTGCACCTCC
  TGAAGTCCAGCTGTGCCCCTAGTGTCCAGATGAAGATCAAAGAGCTTTACCGACGACGCT
  TTCCCCGGAAGACCCTGGGGCCCTCTGATCTCTCCCTTCTCTCTTTGCCCCCTGGCACCT
  CTCCTGTAGGCTCCCCTGGTCCTCTAGCTCCCATTCCCCCAACGCTGTTGGCCCCTGGCA
  CCCTGCTGGGCCCCAAGCGTGAGGTGGACATGCACCCCCCTCTGCCCCAGCCTGTGCACC
  CTGATGTCACCATGAAACCATTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGGCCCA
  CCACCCTTGCATCCACTTCTAGCCAGCGGTTTGAGGAAGCGCACTTTACCTTTGCCCTCA
  CACCCCAGCAAGTGCAGCAGATTCTTACATCCAGAGAGGTTCTGCCAGGAGCCAAATGTG
  ATTATACCATACAGGTGCAGCTAAGGTTCTGTCTCTGTGAGACCAGCTGCCCCCAGGAAG
  ATTATTTTCCCCCCAACCTCTTTGTCAAGGTCAATGGGAAACTGTGCCCCCTGCCGGGTT
  ACCTTCCCCCAACCAAGAATGGGGCCGAGCCCAAGAGGCCCAGCCGCCCCATCAACATCA
  CACCCCTGGCTCGACTCTCAGCCACTGTTCCCAACACCATTGTGGTCAATTGGTCATCTG
  AGTTCGGACGGAATTACTCCTTGTCTGTGTACCTGGTGAGGCAGTTGACTGCAGGAACCC
  TTCTACAAAAACTCAGAGCAAAGGGTATCCGGAACCCAGACCACTCGCGGGCACTGATCA
  AGGAGAAATTGACTGCTGACCCTGACAGTGAGGTGGCCACTACAAGTCTCCGGGTGTCAC
  TCATGTGCCCGCTAGGGAAGATGCGCCTGACTGTCCCTTGTCGTGCCCTCACCTGCGCCC
  ACCTGCAGAGCTTCGATGCTGCCCTTTATCTACAGATGAATGAGAAGAAGCCTACATGGA
  CATGTCCTGTGTGTGACAAGAAGGCTCCCTATGAATCTCTTATCATTGATGGTTTATTTA
  TGGAGATTCTTAGTTCCTGTTCAGATTGTGATGAGATCCAATTCATGGAAGATGGATCCT
  GGTGCCCAATGAAACCCAAGAAGGAGGCATCTGAGGTTTGCCCCCCGCCAGGGTATGGGC
  TGGATGGCCTCCAGTACAGCCCAGTCCAGGGGGGAGATCCATCAGAGAATAAGAAGAAGG
  TCGAAGTTATTGACTTGACAATAGAAAGCTCATCAGATGAGGAGGATCTGCCCCCTACCA
  AGAAGCACTGTTCTGTCACCTCAGCTGCCATCCCGGCCCTACCTGGAAGCAAAGGAGTCC
  TGACATCTGGCCACCAGCCATCCTCGGTGCTAAGGAGCCCTGCTATGGGCACGTTGGGTG
  GGGATTTCCTGTCCAGTCTCCCACTACATGAGTACCCACCTGCCTTCCCACTGGGAGCCG
  ACATCCAAGGTTTAGATTTATTTTCATTTCTTCAGACAGAGAGTCAGCACTATGGCCCCT
  CTGTCATCACCTCACTAGATGAACAGGATGCCCTTGGCCACTTCTTCCAGTACCGAGGGA
  CCCCTTCTCACTTTCTGGGCCCACTGGCCCCCACGCTGGGGAGCTCCCACTGCAGCGCCA
  CTCCGGCGCCCCCTCCTGGCCGTGTCAGCAGCATTGTGGCCCCTGGGGGGGCCTTGAGGG
  AGGGGCATGGAGGACCCCTGCCCTCAGGTCCCTCTTTGACTGGCTGTCGGTCAGACATCA
  TTTCCCTGGACTGAGTTCCCTGGATTATGGAAACTTCGCTGTCCCCCAACACTGAGCAAG
  TATGCTGTGGAGTCCCAACCCCAGCTACTCTGATCCCTCTGGGGGCTCTGGCCAAGGGCC
  AGACAGACCTTCACAGATGCCTACTTTTGGCCTCATCTCTGCCTGACAAGGCCAGCACCC
  AAAGGGTTAATATTTAACCTCTTTTTAAGGACACTGGGGTCTGTTTCTGGAAATGTTCTT
  TAGATGGTGGCACATTCCTTTGGGTATGTTAACCTAGGCAGTGGGAGGCAAATGGGATGG
  TATGTGAGCTAGGAGAAGGGCTGAACCCTCAGCCTTGACTATGTCTAGAGCCTCTTGGGG
  AAGGGGCACCTCTCTTGAACCCCAAATGCTCTCTCTTCTTATTACCCAAACCCATGGCTC
  TATTTCTTCTTCACATCCATTGTCTCTTCATGTCTATTCCATTCCCTTCGGCCAAACAGA
  CAGGTGGAAAAACTGAGACAGGCAGTTTCAGAGATGGACAGAGAACTTTATTTTGGATTG
  TGGATGTGGACTTTTTTGTACATAAATAAGAAAAACCAAAATACTCCAAAGATGACTTCC
  CCTGCCTCCTACTCCAGTATGACAGAGGAGGATGTAAGGCCTTAGCCATGATCTGCAGGG
  GTCTGGGAGTCAGGCCCGGCCTATTGCTTGGGTCTCTCTCTATTTATATATCTAAGTTCA
  CAGTGTTTCTTATTCCCCCCTAAGCTTCTAGAGGCTCATGGCCCTGTAGTTAGGCCTGGC
  TCATTCTGCACCTTTCCAGGGAGGTGGAAGGACCCTGTGCCCTCCTTCCCAATCTTCTTT
  TTCAGGCTCGCCAAGGCCTAGGACCTATGTTGTAATTTTACTTTTTATTTCTAAAGTTGT
  AGTGAAGCTCTCACCCATAATAAAGGTTGTGAATGTTCAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006099
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006099.3, NP_006090.2
• RefSeq Size: 2902 bp
• RefSeq ORF: 1887 bp
• Locus ID: 10401
• UniProt ID: Q9Y6X2
• Cytogenetics: 1q21.1
• Domains: SAP, zf-MIZ
• Protein Families: Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]