PIAS3 (NM_006099) Human Untagged Clone

CAT#: SC116323

PIAS3 (untagged)-Human protein inhibitor of activated STAT, 3 (PIAS3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIAS3
• Synonyms: ZMIZ5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006099, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGAGCTGGGCGAATTAAAGCACATGGTGATGAGTTTCCGGGTGTCTGAGCTCCAGGTGCTTCTTG
  GCTTTGCTGGCCGGAACAAGAGTGGACGGAAGCACGAGCTCCTGGCCAAGGCTCTGCACCTCCTGAAGTC
  CAGCTGTGCCCCTAGTGTCCAGATGAAGATCAAAGAGCTTTACCGACGACGCTTTCCCCGGAAGACCCTG
  GGGCCCTCTGATCTCTCCCTTCTCTCTTTGCCCCCTGGCACCTCTCCTGTAGGCTCCCCTGGTCCTCTAG
  CTCCCATTCCCCCAACGCTGTTGGCCCCTGGCACCCTGCTGGGCCCCAAGCGTGAGGTGGACATGCACCC
  CCCTCTGCCCCAGCCTGTGCACCCTGATGTCACCATGAAACCATTGCCCTTCTATGAAGTCTATGGGGAG
  CTCATCCGGCCCACCACCCTTGCATCCACTTCTAGCCAGCGGTTTGAGGAAGCGCACTTTACCTTTGCCC
  TCACACCCCAGCAAGTGCAGCAGATTCTTACATCCAGAGAGGTTCTGCCAGGAGCCAAATGTGATTATAC
  CATACAGGTGCAGCTAAGGTTCTGTCTCTGTGAGACCAGCTGCCCCCAGGAAGATTATTTTCCCCCCAAC
  CTCTTTGTCAAGGTCAATGGGAAACTGTGCCCCCTGCCGGGTTACCTTCCCCCAACCAAGAATGGGGCCG
  AGCCCAAGAGGCCCAGCCGCCCCATCAACATCACACCCCTGGCTCGACTCTCAGCCACTGTTCCCAACAC
  CATTGTGGTCAATTGGTCATCTGAGTTCGGACGGAATTACTCCTTGTCTGTGTACCTGGTGAGGCAGTTG
  ACTGCAGGAACCCTTCTACAAAAACTCAGAGCAAAGGGTATCCGGAACCCAGACCACTCGCGGGCACTGA
  TCAAGGAGAAATTGACTGCTGACCCTGACAGTGAGGTGGCCACTACAAGTCTCCGGGTGTCACTCATGTG
  CCCGCTAGGGAAGATGCGCCTGACTGTCCCTTGTCGTGCCCTCACCTGCGCCCACCTGCAGAGCTTCGAT
  GCTGCCCTTTATCTACAGATGAATGAGAAGAAGCCTACATGGACATGTCCTGTGTGTGACAAGAAGGCTC
  CCTATGAATCTCTTATCATTGATGGTTTATTTATGGAGATTCTTAGTTCCTGTTCAGATTGTGATGAGAT
  CCAATTCATGGAAGATGGATCCTGGTGCCCAATGAAACCCAAGAAGGAGGCATCTGAGGTTTGCCCCCCG
  CCAGGGTATGGGCTGGATGGCCTCCAGTACAGCCCAGTCCAGGGGGGAGATCCATCAGAGAATAAGAAGA
  AGGTCGAAGTTATTGACTTGACAATAGAAAGCTCATCAGATGAGGAGGATCTGCCCCCTACCAAGAAGCA
  CTGTTCTGTCACCTCAGCTGCCATCCCGGCCCTACCTGGAAGCAAAGGAGTCCTGACATCTGGCCACCAG
  CCATCCTCGGTGCTAAGGAGCCCTGCTATGGGCACGTTGGGTGGGGATTTCCTGTCCAGTCTCCCACTAC
  ATGAGTACCCACCTGCCTTCCCACTGGGAGCCGACATCCAAGGTTTAGATTTATTTTCATTTCTTCAGAC
  AGAGAGTCAGCACTATGGCCCCTCTGTCATCACCTCACTAGATGAACAGGATGCCCTTGGCCACTTCTTC
  CAGTACCGAGGGACCCCTTCTCACTTTCTGGGCCCACTGGCCCCCACGCTGGGGAGCTCCCACTGCAGCG
  CCACTCCGGCGCCCCCTCCTGGCCGTGTCAGCAGCATTGTGGCCCCTGGGGGGGCCTTGAGGGAGGGGCA
  TGGAGGACCCCTGCCCTCAGGTCCCTCTTTGACTGGCTGTCGGTCAGACATCATTTCCCTGGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006099 unedited
  TATATTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCCCCATTCCCC
  CACCTCTCCAGCTCGCCCTCTGAGCCTCCCGAGCCCTCTCTCCATTTCCCACAATTGTGC
  TGCACATGGTGATGAGTTTCCGGGTGTCTGAGCTCCAGGTGCTTCTTGGCTTTGCTGGCC
  GGAACAAGAGTGGACGGAAGCACGAGCTCCTGGCCAAGGCTCTGCACCTCCTGAAGTCCA
  GCTGTGCCCCTAGTGTCCAGATGAAGATCAAAGAGCTTTACCGACGACGCTTTCCCCGGA
  AGACCCTGGGGCCCTCTGATCTCTCCCTTCTCTCTTTGCCCCCTGGCACCTCTCCTGTAG
  GCTCCCCTGGTCCTCTAGCTCCCATTCCCCCAACGCTGTTGGCCCCTGGCACCCTGCTGG
  GCCCCAAGCGTGAGGTGGACATGCACCCCCCTCTGCCCCAGCCTGTGCACCCTGATGTCA
  CCATGAAACCATTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGGCCCACCACCCTTG
  CATCCACTTCTAGCCAGCGGTTTGAGGAAGCGCACTTTACCTTTGCCCTCACACCCCAGC
  AAGTGCAGCAGATTCTTACATCCAGAGAGGTTCTGCCAGGAGCCAAATGTGATTATACCA
  TACAGGTGCAGCTAAGGTTCTGTCTCTGTGAGACCAGCTGCCCCCAGNAAGATTATTTTC
  CCCCCAACCTCTNTGTCAAGGTCAATGGGAAACTGTGCCCNNCTGCCGGTTACCTTCCCC
  CACCAAGAATGGNGCCGAGCCCCAGAAGCCCAGCCGNCCCCATCACATCACACCCCTGGC
  TCGACTCTCAGNCCTGGTCCCACACCATTTGTGTCATGGNTCATCTGAGTTCGNACGGAA
  ATACTCTTGTCTGTGTACCTGGNGAGGCAGNTGACTGCAGGAACCCTTCTACAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006099 unedited
  GCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAACATTCA
  CAACCTTTATTATGGGTGAGAGCTTCACTACAACTTTAGAAATAAAAAGTAAAATTACAA
  CATAGGTCCTAAGCCTTGGCGAGCCTGAAAAAGAAGATTGGGAAGGAGGGCACAGGGTCC
  TTCCACCTCCCTGGAAAGGTGCAGAATGAGCCAGGCCTAACTACAGGGCCATGAGCCTCT
  AAAAGCTTAGGGGGGAATAAGAAACACTGGGAACTTAGATATATAAATAGAGAGAGACCC
  AAGCAATAGGCCGGGCCTGACTCCCAGACCCCTGCAGATCATGGCTAAGGCCTTACATCC
  TCCTCTGTCATACTGGAGTAGGAGGCAGGGGAAGTCATCTTTGGAGTATTTTGGTTTTTC
  TTATTTATGTACAAAAAAGTCCACATCCACAATCCAAAATAAAGTTCTCTGTCCATCTCT
  GAAACTGCCTGTCTCAGTTTTTCCACCTGTCTGTTTGGCCGAAGGGAATGGAATATACAT
  GAAGAGACAATGGATGTGAAGAAGAAATAGAGCCATGGGTTCGGGTAATAAGAAGAGAGA
  GCTTTTGGGGTTCAAGAGAGGTGCCCCTTCCCCAAGAGGCTCTAGACATAATCAAGGCTG
  AAGGTTCAACCCCTTTTCTAGCTCAATACCATCCCATTTGCCTCCCACTGCCTAGGTAAA
  CTACCCAAGGAATGTGCCCCCCTCTAAAGACATTTCCAGAACAGACCCAAGGGCTTAAAA
  GAGGCTAAATTTAACCCTTTGGTGCTGGCCTTTCCGGCAAAATAGCCCAAAGTAGGTTTC
  TGGAAGGCTTCTGGCCCTGGGCACACCCCCAAGGAACATAGAACTGGGTTGGACCCCCAA
  TACTTCCTCAAGGTGGGCAACAAAATTTCCTACCCGGGACCCTTCGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006099
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006099.2, NP_006090.1
• RefSeq Size: 2902 bp
• RefSeq ORF: 1860 bp
• Locus ID: 10401
• UniProt ID: Q9Y6X2
• Cytogenetics: 1q21.1
• Domains: SAP, zf-MIZ
• Protein Families: Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]