RED1 (ADARB1) (NM_001112) Human Untagged Clone

CAT#: SC321955

ADARB1 (untagged)-Human adenosine deaminase, RNA-specific, B1 (ADARB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RED1
• Synonyms: ADAR2; DRABA2; DRADA2; NEDHYMS; RED1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC321955 CGAAGCGTGGGGCGCGCGTGCGGAGGACCAGGCGCGGCGCGGCTGCGGCTGAGAGTGGAG
  CCTTTCAGGCTGGCATGGAGAGCTTAAGGGGCAACTGAAGGAGACACACTGGCCAAGCGC
  GGAGTTCTGCTTACTTCAGTCCTGCTGAGATACTCTCTCAGTCCGCTCGCACCGAAGGAA
  GCTGCCTTGGGATCAGAGCAGACATAAAGCTAGAAAAATTTCAAGACAGAAACAGTCTCC
  GCCAGTCAAGAAACCCTCAAAAGTATTTTGCCATGGATATAGAAGATGAAGAAAACATGA
  GTTCCAGCAGCACTGATGTGAAGGAAAACCGCAATCTGGACAACGTGTCCCCCAAGGATG
  GCAGCACACCTGGGCCTGGCGAGGGCTCTCAGCTCTCCAATGGGGGTGGTGGTGGCCCCG
  GCAGAAAGCGGCCCCTGGAGGAGGGCAGCAATGGCCACTCCAAGTACCGCCTGAAGAAAA
  GGAGGAAAACACCAGGGCCCGTCCTCCCCAAGAACGCCCTGATGCAGCTGAATGAGATCA
  AGCCTGGTTTGCAGTACACACTCCTGTCCCAGACTGGGCCCGTGCACGCGCCTTTGTTTG
  TCATGTCTGTGGAGGTGAATGGCCAGGTTTTTGAGGGCTCTGGTCCCACAAAGAAAAAGG
  CAAAACTCCATGCTGCTGAGAAGGCCTTGAGGTCTTTCGTTCAGTTTCCTAATGCCTCTG
  AGGCCCACCTGGCCATGGGGAGGACCCTGTCTGTCAACACGGACTTCACATCTGACCAGG
  CCGACTTCCCTGACACGCTCTTCAATGGTTTTGAAACTCCTGACAAGGCGGAGCCTCCCT
  TTTACGTGGGCTCCAATGGGGATGACTCCTTCAGTTCCAGCGGGGACCTCAGCTTGTCTG
  CTTCCCCGGTGCCTGCCAGCCTAGCCCAGCCTCCTCTCCCTGCCTTACCACCATTCCCAC
  CCCCGAGTGGGAAGAATCCCGTGATGATCTTGAACGAACTGCGCCCAGGACTCAAGTATG
  ACTTCCTCTCCGAGAGCGGGGAGAGCCATGCCAAGAGCTTCGTCATGTCTGTGGTCGTGG
  ATGGTCAGTTCTTTGAAGGCTCGGGGAGAAACAAGAAGCTTGCCAAGGCCCGGGCTGCGC
  AGTCTGCCCTGGCCGCCATTTTTAACTTGCACTTGGATCAGACGCCATCTCGCCAGCCTA
  TTCCCAGTGAGGGTCTTCAGCTGCATTTACCGCAGGTTTTAGCTGACGCTGTCTCACGCC
  TGGTCCTGGGTAAGTTTGGTGACCTGACCGACAACTTCTCCTCCCCTCACGCTCGCAGAA
  AAGTGCTGGCTGGAGTCGTCATGACAACAGGCACAGATGTTAAAGATGCCAAGGTGATAA
  GTGTTTCTACAGGAACAAAATGTATTAATGGTGAATACATGAGTGATCGTGGCCTTGCAT
  TAAATGACTGCCATGCAGAAATAATATCTCGGAGATCCTTGCTCAGATTTCTTTATACAC
  AACTTGAGCTTTACTTAAATAACAAAGATGATCAAAAAAGATCCATCTTTCAGAAATCAG
  AGCGAGGGGGGTTTAGGCTGAAGGAGAATGTCCAGTTTCATCTGTACATCAGCACCTCTC
  CCTGTGGAGATGCCAGAATCTTCTCACCACATGAGCCAATCCTGGAAGAACCAGCAGATA
  GACACCCAAATCGTAAAGCAAGAGGACAGCTACGGACCAAAATAGAGTCTGGTGAGGGGA
  CGATTCCAGTGCGCTCCAATGCGAGCATCCAAACGTGGGACGGGGTGCTGCAAGGGGAGC
  GGCTGCTCACCATGTCCTGCAGTGACAAGATTGCACGCTGGAACGTGGTGGGCATCCAGG
  GATCCCTGCTCAGCATTTTCGTGGAGCCCATTTACTTCTCGAGCATCATCCTGGGCAGCC
  TTTACCACGGGGACCACCTTTCCAGGGCCATGTACCAGCGGATCTCCAACATAGAGGACC
  TGCCACCTCTCTACACCCTCAACAAGCCTTTGCTCAGTGGCATCAGCAATGCAGAAGCAC
  GGCAGCCAGGGAAGGCCCCCAACTTCAGTGTCAACTGGACGGTAGGCGACTCCGCTATTG
  AGGTCATCAACGCCACGACTGGGAAGGATGAGCTGGGCCGCGCGTCCCGCCTGTGTAAGC
  ACGCGTTGTACTGTCGCTGGATGCGTGTGCACGGCAAGGTTCCCTCCCACTTACTACGCT
  CCAAGATTACCAAACCCAACGTGTACCATGAGTCCAAGCTGGCGGCAAAGGAGTACCAGG
  CCGCCAAGGCGCGTCTGTTCACAGCCTTCATCAAGGCGGGGCTGGGGGCCTGGGTGGAGA
  AGCCCACCGAGCAGGACCAGTTCTCACTCACGCCCTGACCCGGGCAGACATGATGGGGGG
  TGCAGGGGGCTGTGGGCATCCAGCGTCATCCTCCAGAACCTCACATCTGAACTGGGGGCA
  GGTGCATACCTTGGGGAGGGAGTAGGGGGACACGGGGGACCACCAGGTGTCCACGGTTGT
  CCCCAGCATCTCACATCAGACCTGGGGCAGGTGCGCAGTGTGGGGAGGGGATGGGGTGCG
  TCAGGGCCCAGCATCGCCGCCTGGCATCTCTCTGCCGCAGCATTTCCCCTTCTGAACCGT
  CCAGTGACTGCTTTCAATCTCGGTTTACGTTTAGAAATTGAGTTCTACTGAGTAGGGCTT
  CCTTAAGTTTAGGAAAATAGAAATTACTTTGTGTGAAATTCTTGAATAAATAATTTATTC
  AGAGCTAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001112
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001112.2, NP_001103.1
• RefSeq Size: 6881 bp
• RefSeq ORF: 2106 bp
• Locus ID: 104
• UniProt ID: P78563
• Cytogenetics: 21q22.3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1), also known as ADAR2a-L1 or DRADA2a, lacks the ALU cassette insert and contains the long C-terminal region, as compared to variant 2. The resulting isoform (1), also known as hRED1-Short, lacks an internal segment, compared to isoform 2.