GNS (NM_002076) Human Untagged Clone

CAT#: SC321684

GNS (untagged)-Human glucosamine (N-acetyl)-6-sulfatase (GNS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GNS
• Synonyms: G6S
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002076.2 CGTCTGACTCCAGCCACCGGCCTTCAAGGCACGGCTTTTTATTCCTTCGGCTGGTCGGCC
  TCTCGCCCTTCAGCTACCTGTGCGTCCCTCCGTCCCGTCCCGTCCCGGGGTCACCCCGGA
  GCCTGTCCGCTATGCGGCTCCTGCCTCTAGCCCCAGGTCGGCTCCGGCGGGGCAGCCCCC
  GCCACCTGCCCTCCTGCAGCCCAGCGCTGCTACTGCTGGTGCTGGGCGGCTGCCTGGGGG
  TCTTCGGGGTGGCTGCGGGAACCCGGAGGCCCAACGTGGTGCTGCTCCTCACGGACGACC
  AGGACGAAGTGCTCGGCGGCATGACACCGCTAAAGAAAACCAAAGCTCTCATCGGAGAGA
  TGGGGATGACTTTTTCCAGTGCTTATGTGCCAAGTGCTCTCTGCTGCCCCAGCAGAGCCA
  GTATCCTGACAGGAAAGTACCCACATAATCATCACGTTGTGAACAACACTCTGGAGGGGA
  ACTGCAGTAGTAAGTCCTGGCAGAAGATCCAAGAACCAAATACTTTCCCAGCAATTCTCA
  GATCAATGTGTGGTTATCAGACCTTTTTTGCAGGGAAATATTTAAATGAGTACGGAGCCC
  CAGATGCAGGTGGACTAGAACACGTTCCTCTGGGTTGGAGTTACTGGTATGCCTTGGAAA
  AGAATTCTAAGTATTATAATTACACCCTGTCTATCAATGGGAAGGCACGGAAGCATGGTG
  AAAACTATAGTGTGGACTACCTGACAGATGTTTTGGCTAATGTCTCCTTGGACTTTCTGG
  ACTACAAGTCCAACTTTGAGCCCTTCTTCATGATGATCGCCACTCCAGCGCCTCATTCGC
  CTTGGACAGCTGCACCTCAGTACCAGAAGGCTTTCCAGAATGTCTTTGCACCAAGAAACA
  AGAACTTCAACATCCATGGAACGAACAAGCACTGGTTAATTAGGCAAGCCAAGACTCCAA
  TGACTAATTCTTCAATACAGTTTTTAGATAATGCATTTAGGAAAAGGTGGCAAACTCTCC
  TCTCAGTTGATGACCTTGTGGAGAAACTGGTCAAGAGGCTGGAGTTCACTGGGGAGCTCA
  ACAACACTTACATCTTCTATACCTCAGACAATGGCTATCACACAGGACAGTTTTCCTTGC
  CAATAGACAAGAGACAGCTGTATGAGTTTGATATCAAAGTTCCACTGTTGGTTCGAGGAC
  CTGGGATCAAACCAAATCAGACAAGCAAGATGCTGGTTGCCAACATTGACTTGGGTCCTA
  CTATTTTGGACATTGCTGGCTACGACCTAAATAAGACACAGATGGATGGGATGTCCTTAT
  TGCCCATTTTGAGAGGTGCCAGTAACTTGACCTGGCGATCAGATGTCCTGGTGGAATACC
  AAGGAGAAGGCCGTAACGTCACTGACCCAACATGCCCTTCCCTGAGTCCTGGCGTATCTC
  AATGCTTCCCAGACTGTGTATGTGAAGATGCTTATAACAATACCTATGCCTGTGTGAGGA
  CAATGTCAGCATTGTGGAATTTGCAGTATTGCGAGTTTGATGACCAGGAGGTGTTTGTAG
  AAGTCTATAATCTGACTGCAGACCCAGACCAGATCACTAACATTGCTAAAACCATAGACC
  CAGAGCTTTTAGGAAAGATGAACTATCGGTTAATGATGTTACAGTCCTGTTCTGGGCCAA
  CCTGTCGCACTCCAGGGGTTTTTGACCCCGGATACAGGTTTGACCCCCGTCTCATGTTCA
  GCAATCGCGGCAGTGTCAGGACTCGAAGATTTTCCAAACATCTTCTGTAGCGACCTCACA
  CAGCCTCTGCAGATGGATCCCTGCACGCCTCTTTCTGATGAAGTGATTGTAGTAGGTGTC
  TGTAGCTAGTCTTCAAGACCACACCTGGAAGAGTTTCTGGGCTGGCTTTAAGTCCTGTTT
  GAAAAAGCAACCCAGTCAGCTGACTTCCTCGTGCAATGTGTTAAACTGTGAACTCTGCCC
  ATGTGTCAGGAGTGGCTGTCTCTGGTCTCTTCCTTTAGCTGACAAGGACACTCCTGAGGT
  CTTTGTTCTCACTGTATTCTTTTTATCCTGGGGCCACAGTTCTTGATTATTCCTCTTGTG
  GTTAAAGACTGAATTTGTAAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002076
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002076.2, NP_002067.1
• RefSeq Size: 5130 bp
• RefSeq ORF: 1659 bp
• Locus ID: 2799
• UniProt ID: P15586
• Cytogenetics: 12q14.3
• Domains: Sulfatase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome. [provided by RefSeq, Jul 2008]