NCBP1 (NM_002486) Human Untagged Clone

CAT#: SC321555

NCBP1 (untagged)-Human nuclear cap binding protein subunit 1, 80kDa (NCBP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCBP1
• Synonyms: CBP80; NCBP; Sto1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002486.4 CCAGACAGTTCCTGCAGCGCTTACCGCCTGGCCTCTCGGTTCCGCGGCGCACCGGAGGGC
  AGCATGTCGCGGCGGCGGCACAGCGACGAGAACGACGGTGGGCAGCCTCACAAAAGGAGA
  AAGACCTCTGATGCAAATGAAACTGAAGATCATTTGGAATCTTTAATATGTAAAGTAGGA
  GAAAAGAGTGCCTGCTCTTTGGAGAGCAACCTAGAAGGCTTGGCTGGTGTTTTGGAAGCT
  GATCTTCCTAACTACAAGAGCAAGATCTTAAGGCTTCTTTGTACAGTTGCACGCCTATTA
  CCTGAGAAGCTGACAATTTATACAACATTAGTTGGACTACTGAATGCCAGGAATTACAAT
  TTTGGTGGAGAATTTGTAGAAGCCATGATTCGTCAACTTAAAGAATCATTGAAAGCAAAC
  AATTATAATGAAGCCGTGTATTTGGTCCGTTTTTTATCTGATCTTGTGAATTGTCATGTG
  ATTGCCGCCCCATCAATGGTTGCTATGTTTGAAAATTTTGTAAGCGTAACTCAGGAAGAA
  GATGTACCTCAGGTGCGACGAGATTGGTATGTGTATGCATTTCTGTCATCTTTGCCCTGG
  GTTGGAAAGGAGTTGTACGAAAAGAAAGATGCAGAGATGGACCGCATCTTTGCCAACACT
  GAAAGCTATCTTAAAAGACGCCAAAAGACTCATGTACCCATGTTACAGGTATGGACTGCT
  GATAAACCACATCCACAAGAAGAGTATTTAGATTGCCTGTGGGCCCAGATTCAGAAATTG
  AAAAAGGATCGCTGGCAGGAACGGCACATCCTAAGACCTTATCTTGCCTTTGACAGCATC
  CTGTGTGAAGCACTGCAGCACAATCTGCCTCCTTTTACACCACCTCCTCACACTGAAGAT
  TCAGTGTACCCAATGCCAAGGGTCATCTTCAGAATGTTTGATTACACAGATGATCCCGAG
  GGTCCTGTCATGCCAGGGAGTCATTCAGTGGAAAGATTTGTAATAGAAGAGAATCTTCAC
  TGCATCATTAAGTCCCACTGGAAGGAAAGGAAGACTTGTGCTGCACAGTTAGTGAGCTAT
  CCAGGGAAGAACAAGATCCCCTTGAACTACCACATAGTTGAGGTGATCTTTGCAGAGCTG
  TTTCAACTTCCAGCACCCCCTCACATTGATGTGATGTACACAACACTCCTCATTGAACTG
  TGCAAACTTCAACCTGGCTCTCTACCCCAAGTTCTTGCACAGGCAACTGAAATGCTATAC
  ATGCGTTTGGACACAATGAACACTACCTGTGTAGACAGGTTTATTAATTGGTTTTCTCAT
  CATCTAAGTAACTTCCAGTTCCGTTGGAGCTGGGAAGATTGGTCAGATTGTCTTAGTCAA
  GATCCTGAAAGTCCCAAACCGAAGTTTGTAAGAGAAGTTCTAGAAAAATGTATGAGGTTG
  TCTTACCATCAGCGTATATTAGATATTGTTCCTCCTACCTTCTCAGCTCTGTGTCCTGCA
  AACCCAACCTGCATTTACAAGTATGGAGATGAAAGTAGCAATTCTCTTCCTGGACATTCT
  GTTGCCCTCTGTTTAGCTGTTGCCTTTAAAAGTAAGGCAACCAATGATGAAATCTTCAGC
  ATTCTGAAAGATGTACCAAATCCTAACCAGGATGATGACGACGATGAAGGATTCAGTTTT
  AACCCATTGAAAATAGAAGTCTTTGTACAGACTCTGCTACACTTGGCAGCCAAATCATTC
  AGCCACTCCTTCAGTGCTCTTGCAAAGTTTCATGAAGTCTTCAAAACCCTAGCTGAAAGT
  GATGAAGGAAAGTTACATGTGCTAAGAGTTATGTTTGAGGTCTGGAGGAACCATCCACAG
  ATGATTGCTGTACTAGTGGATAAGATGATTCGTACACAAATAGTTGATTGTGCTGCCGTA
  GCAAATTGGATCTTCTCTTCAGAACTATCTCGTGACTTTACCAGATTGTTTGTTTGGGAA
  ATTTTGCACTCTACAATTCGTAAGATGAACAAACATGTCCTGAAGATCCAGAAAGAGCTG
  GAAGAAGCTAAAGAGAAACTTGCTAGGCAACACAAACGGCGAAGTGATGATGACGACAGA
  AGCAGTGACAGGAAAGACGGGGTTCTTGAGGAACAAATAGAACGACTTCAGGAAAAAGTG
  GAATCTGCTCAGAGTGAACAAAAGAATCTTTTCCTCGTTATATTTCAGCGGTTTATCATG
  ATCTTGACCGAGCACCTAGTACGATGCGAAACTGATGGGACCAGTGTATTAACACCATGG
  TATAAGAACTGTATAGAGAGGCTGCAGCAGATCTTCCTACAGCATCACCAAATAATCCAG
  CAGTACATGGTGACCCTGGAGAACCTTCTCTTCACTGCTGAATTAGACCCTCATATCTTG
  GCCGTGTTCCAGCAGTTCTGTGCCCTGCAGGCCTAAGGGTCATTTTTTCCTCATGTCAAG
  GTTTTTTTTGATATCTTAAAATAATTTGTCTTATAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002486
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002486.4, NP_002477.1
• RefSeq Size: 5381 bp
• RefSeq ORF: 2373 bp
• Locus ID: 4686
• UniProt ID: Q09161
• Cytogenetics: 9q22.33
• Domains: MIF4G
• Protein Pathways: Spliceosome
• Gene Summary: The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq, Jul 2008]