LAT2 (SLC7A8) (NM_012244) Human Untagged Clone

CAT#: SC321483

SLC7A8 (untagged)-Human solute carrier family 7 (amino acid transporter, L-type), member 8 (SLC7A8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LAT2
• Synonyms: LAT2; LPI-PC1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012244.2 CTTTTGAGGTTGCAATCCTACTGAGAAGGATGGAAGAAGGAGCCAGGCACCGAAACAACA
  CCGAAAAGAAACACCCAGGTGGGGGCGAGTCGGACGCCAGCCCCGAGGCTGGTTCCGGAG
  GGGGCGGAGTAGCCCTGAAGAAAGAGATCGGATTGGTCAGTGCCTGTGGTATCATCGTAG
  GGAACATCATCGGCTCTGGAATCTTTGTCTCGCCAAAGGGAGTGCTGGAGAATGCTGGTT
  CTGTGGGCCTTGCTCTCATCGTCTGGATTGTGACGGGCTTCATCACAGTTGTGGGAGCCC
  TCTGCTATGCTGAACTCGGGGTCACCATCCCCAAATCTGGAGGTGACTACTCCTATGTCA
  AGGACATCTTCGGAGGACTGGCTGGGTTCCTGAGGCTGTGGATTGCTGTGCTGGTGATCT
  ACCCCACCAACCAGGCTGTCATCGCCCTCACCTTCTCCAACTACGTGCTGCAGCCGCTCT
  TCCCCACCTGCTTCCCCCCAGAGTCTGGCCTTCGGCTCCTGGCTGCCATCTGCTTATTGC
  TCCTCACATGGGTCAACTGTTCCAGTGTGCGGTGGGCCACCCGGGTTCAAGACATCTTCA
  CAGCTGGGAAGCTCCTGGCCTTGGCCCTGATTATCATCATGGGGATTGTACAGATATGCA
  AAGGAGAGTACTTCTGGCTGGAGCCAAAGAATGCATTTGAGAATTTCCAGGAACCTGACA
  TCGGCCTCGTCGCACTGGCTTTCCTTCAGGGCTCCTTTGCCTATGGAGGCTGGAACTTTC
  TGAATTACGTGACTGAGGAGCTTGTTGATCCCTACAAGAACCTTCCCAGAGCCATCTTCA
  TCTCCATCCCACTGGTCACATTTGTGTATGTCTTTGCCAATGTCGCTTATGTCACTGCAA
  TGTCCCCCCAGGAGCTGCTGGCATCCAACGCCGTCGCTGTGACTTTTGGAGAGAAGCTCC
  TAGGAGTCATGGCCTGGATCATGCCCATTTCTGTTGCCCTGTCCACATTTGGAGGAGTTA
  ATGGGTCTCTCTTCACCTCCTCTCGGCTGTTCTTCGCTGGAGCCCGAGAGGGCCACCTTC
  CCAGTGTGTTGGCCATGATCCACGTGAAGCGCTGCACCCCAATCCCAGCCCTGCTCTTCA
  CATGCATCTCCACCCTGCTGATGCTGGTCACCAGTGACATGTACACACTCATCAACTACG
  TGGGCTTCATCAACTACCTCTTCTATGGGGTCACGGTTGCTGGACAGATAGTCCTTCGCT
  GGAAGAAGCCTGATATCCCCCGCCCCATCAAGATCAACCTGCTGTTCCCCATCATCTACT
  TGCTGTTCTGGGCCTTCCTGCTGGTCTTCAGCCTGTGGTCAGAGCCGGTGGTGTGTGGCA
  TTGGCCTGGCCATCATGCTGACAGGAGTGCCTGTCTATTTCCTGGGTGTTTACTGGCAAC
  ACAAGCCCAAGTGTTTCAGTGACTTCATTGAGCTGCTAACCCTGGTGAGCCAGAAGATGT
  GTGTGGTCGTGTACCCCGAGGTGGAGCGGGGCTCAGGGACAGAGGAGGCTAATGAGGACA
  TGGAGGAGCAGCAGCAGCCCATGTACCAACCCACTCCCACGAAGGACAAGGACGTGGCGG
  GGCAGCCCCAGCCCTGAGGACCACCATTCCCTGGCTACTCTCTCCTTCCTCCCCCTTTTA
  TCCTACCTCCCTGCCTTGGTCCCGCCAACACATGCGAGTACACACACACCCCTCTCTGCT
  TTTGTCAGGCAGTGGTAGGACTTTGGTGTGGGTGGTGAGAAATTGTAAACAAAACTGACA
  TTCATACCCAAAGAACCAGCCTCTCACCCCAGGGTCCATGTCCCAGGCCCCACTCCAGTG
  CTGCCCACACTCCCAGCTGCTGGAGGAGAGGGGAGATGCCAAGGTGCCCTGCAGGACCTC
  CCTCCGGGCCACACCCTCAGCTGCCTCTTCAGGAACCGGAGCTCATTACTGCCTTCCCTC
  CCAGGGAGGCCCCTTCAGAGAGGAGAGGCCACAGGAGCTGCATTGTGGGGGGACAGGCTC
  AAGCAATTCTGTCCCCATCAAGGGGTCAGCTGGAGAGACCCAAGACCCTATCTGTTCACC
  AGGGACCCAAAATCCAAGGGGATGCTTCCCTCTGCCCTCTTTCCTGCCCCTCCCCATCAT
  ACCTGCACCCACCCCAGCCAGGGCTCCCTGTCCAGAATTCGGTTCTCCTCAGGACGCCAA
  CTCCCAGAGCTAAGGACCAAGGAGAAGAACAGCCTCTCCACCCCCAAGCCAGGCGGTTGA
  GGAACATATTGAGAAAGGTTCAGATTGCAGAAACCCAGCCCTGCCCCTGCCTCCTGCATC
  CAGCCCCCAACATGGTGCCAAAGCTTCCAGAAGCCAAAAAGCTTCTGATTTTTAAGGTAG
  TGGGCATCTCTCTCCTAATGACGAAGCTGCTCAGCAACTCCACCTGCCCGCCGCAGGAAG
  GAGCAGTCCCCTGCTATCCCTGCAGCCACTCCCAGCACACCCGCACACAGCCAGCACCAC
  CGCCCCCACCGTGCACTTCTCCTCTCTGGGCCTTGGCTTGGGACCAGGTACGAAGGATCC
  CCAAGCCCTTCAGGCCTGAGATCAGAGCCAGATCAGCCTTAAGTCACCTCCCATCCAAGA
  ACTTGGCCTAAAAATACTCCCCTATTTCTAACCCTCAGGACGGATCTGATATTAAATGCC
  TTCCCTGGGAGGAAGGGTGCTTTCCCCCTCCCTAGAGGTGCCCATTCCATACCCTGGGAG
  ACTGAGGAGAGCATTGGCTGAAGCCCAGTTCCTTTCCCATCCATCCCCAACTCCAATAAT
  CCCCCACTCCTCGCAGGTCTCAGTGTCATGCTGTCTTGGGGCAGGGTGAAAGGGTAGTGG
  CAGCAGGGCGCCCACTCTGGAGATCCTCAAAAAAGGCCCTCCTCTGTGGCTGGCAGCCTC
  TGACCTTTCCCTGGGCTTCAAAGGAAGGCTATGGAGTTTGCTGTGGGCCCTGCAACCTTC
  CCAGCCACTCCTGCTGCACTAAGGACTTAGGATCCTTTTATCACAAATCGGGATTCTCTC
  CCCCACCCCGAATTCTGTCTGCTTAAACTGGAATACACAGGAGCCCTTCCTGGCCTGGAT
  GGTGTCTCCCAGCTTCCCCGCCCAGCTTGCCCACCCCATAGTTGGTGAGATGCCAAGTTT
  GGTCTGAGTTGTGACCCCTTCAGAGTAGATGCCCGGCAGGCTGGGGTTGGCCCCTGGAGG
  GTCAGGGGACCATCTTCTTATTCCCTCTTTTCTCATTCCTCCAACTTCCTCCCCTCCTTC
  AATTATTTTTTTGTAAAGTTGATGCCTTACTTTTTGGATAAATATTTTTGAAGCTGGTAT
  TTCTATTTCTTTTGGATTTTTTTTAATGTAAGGTTGTTTTGGGGGATGGAGTTAGAACCT
  TAATGATAATTTCTTTCGTTTGGTGTAGGTTTTAGAGATTTGTTTTGTGGAGAGGTTTTT
  TTCTTTTGATGTAATAAAATTTAAAATGGAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_012244
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012244.2, NP_036376.2
• RefSeq Size: 4237 bp
• RefSeq ORF: 1608 bp
• Locus ID: 23428
• UniProt ID: Q9UHI5
• Cytogenetics: 14q11.2
• Domains: aa_permeases
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).