FBXL8 (NM_018378) Human Untagged Clone

CAT#: SC320386

FBXL8 (untagged)-Human F-box and leucine-rich repeat protein 8 (FBXL8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXL8
• Synonyms: FBL8
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018378.2 GCTTCCCGTCCGCTGTCCTCTGCTGCCAGTCCCCTGCCCCGGGCAAAGCCCATCTGGTCC
  GCCGAGCAGGCCGGAGCTATTGGGAGTGGCGGATCCTCCCACCCCAGCCGGATCTGGGCC
  ATGGCCGAGCCTGGAGAGGGACTGCCAGAGGAGGTGCTGGCACTCATCTTCCGCCACCTG
  TCCCTGAGAGACCGTGCTGCCGCCGCCAGGGTCTGCAGGGCCTGGGCCGCCGCTGCTACC
  TGCAGCGCCGTGTGGCACGACACAAAAATCAGTTGCGAATGTGAGCTGGAAGGCATGCTG
  CCACCTTATCTGTCCGCCTGCCTCGACCACATTCACAACCTACGGCTGGAATTTGAGCCA
  TCGAGGAAGCCGAGCCGCCGGGCGGCCATCGAGCTGCTGATGGTTCTGGCGGGCCGTGCC
  CCGGGGCTGCGAGGCCTGCGCCTGGAGTGCCGCGGAGAAAAACCGCTCTTCGACGCGGGC
  CGCGACGTCCTGGAGGCTGTGCACGCTGTATGCGGGGCGGCCAGCCAGCTACGCCACCTC
  GACCTGCGGCGCTTGTCCTTCACACTGGACGACGCGCTGGTGCTGCAGGCGGCGCGCAGC
  TGTCCCGAGCTCCACAGCCTTTTTCTGGACAACAGTACCCTAGTGGGCAGCGTGGGTCCC
  GGCTCAGTGCTCGAGCTACTGGAGGCCTGCCCGCGCCTGCGCGCTCTCGGCCTGCACCTA
  GCCAGTTTGTCGCACGCCATCCTCGAAGCACTGGCGGCGCCAGACCGAGCGCCTTTCGCG
  CTCTTGGCTCTGCGGTGCGCGTGCCCCGAAGATGCACGCGCGTCCCCGCTGCCCAACGAA
  GCCTGGGTCGCGTTGCGCCGCCGCCACCCTGGGCTGGCAGTGGAGCTGGAGCTGGAGCCC
  GCGCTGCCCGCTGAGAGCGTGACGCGCGTCCTGCAGCCAGCCGTCCCCGTGGCTGCGCTG
  CGCCTCAACCTCTCAGGCGACACCGTAGGCCCAGTGCGCTTCGCAGCACACCACTACGCC
  GCAACCCTGTGCGCGCTCGAGGTGCGCGCAGCCGCTTCGGCCGAGCTGAACGCCGCGCTG
  GAGGAGCTGGCGGCGCGCTGCGCGGCCCTGCGCGAGGTGCATTGTTTCTGCGTGGTGAGC
  CACTCGGTGCTGGACGCCTTCCGCGCGCACTGCCCGCGCCTGCGCACCTATACCCTCAAG
  CTCACGCGCGAGCCGCATCCCTGGAGGCCTACGCTCGTGGCGTGATTGGGCGACTTCTCT
  CCCCCGTCCCCGTGGACGTAAGCGCTCTGAGAGGGAACGGGGGGGGGTGTCCAGGCGCGC
  CCCGAGTGCTAGTGCCTTCTTTTGGGATTGTTGCCCCCCGGGTCTTTACCGAGTTGGGAA
  CTGTGATGGCATCGGGACCAGTCCTGGGCGCCCTGAGACCACTCGCTGCTCTCACCCTCT
  GCAGACGCCCCACCCGCTCGGTCCTGGACACACTGCCCCCCTCTCTTGCCTCCACCCCTC
  TGCGGACTCTGCAGCTCCGCGGCCCCGGCGCAGGGAGAGGGAGGGCACGGGCGCGGGCCG
  GGCCTCAAGGGTGAGTGTGAAATAAACAAATCCTGCAGTGAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_018378
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018378.2, NP_060848.2
• RefSeq Size: 1618 bp
• RefSeq ORF: 1125 bp
• Locus ID: 55336
• UniProt ID: Q96CD0
• Cytogenetics: 16q22.1
• Domains: F-box
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. It shares 78% sequence identity with the mouse protein. [provided by RefSeq, Jul 2008]