Phosphatidic acid phosphatase type 2B (PLPP3) (NM_003713) Human Untagged Clone

CAT#: SC320358

PPAP2B (untagged)-Human phosphatidic acid phosphatase type 2B (PPAP2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Phosphatidic acid phosphatase type 2B
• Synonyms: Dri42; LPP3; PAP2B; PPAP2B; VCIP
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003713.3 GGAAGGTGGCCGAGCGCCCGCGCTGCCCACTCGCTCGCTCGCGCACTCAGACGCGCGCCA
  CAACAGCGCGCCCCAAGCTGCGCAGCTCTGCAAAAGTTTCTGCTCGGGATCTGGCTCTCT
  TCCCCTTGGACTTTAGAACGATTTAGGGTTGACAGAGGAAAGCAGAGGCGCGCAGGAGGA
  GCAGAAAACACCACCTTCTGCAGTTGGAGGCAGGCAGCCCCGGCTGCACTCTAGCCGCCG
  CGCCCGGAGCCGGGGCCGACCCGCCACTATCCGCAGCAGCCTCGGCCAGGAGGCGACCCG
  GGCGCCTGGGTGTGTGGCTGCTGTTGCGGGACGTCTTCGCGGGGCGGGAGGCTCGCGCCG
  CAGCCAGCGCCATGCAAAACTACAAGTACGACAAAGCGATCGTCCCGGAGAGCAAGAACG
  GCGGCAGCCCGGCGCTCAACAACAACCCGAGGAGGAGCGGCAGCAAGCGGGTGCTGCTCA
  TCTGCCTCGACCTCTTCTGCCTCTTCATGGCGGGCCTCCCCTTCCTCATCATCGAGACAA
  GCACCATCAAGCCTTACCACCGAGGGTTTTACTGCAATGATGAGAGCATCAAGTACCCAC
  TGAAAACTGGTGAGACAATAAATGACGCTGTGCTCTGTGCCGTGGGGATCGTCATTGCCA
  TCCTCGCGATCATCACGGGGGAATTCTACCGGATCTATTACCTGAAGAAGTCGCGGTCGA
  CGATTCAGAACCCCTACGTGGCAGCACTCTATAAGCAAGTGGGCTGCTTCCTCTTTGGCT
  GTGCCATCAGCCAGTCTTTCACAGACATTGCCAAAGTGTCCATAGGGCGCCTGCGTCCTC
  ACTTCTTGAGTGTCTGCAACCCTGATTTCAGCCAGATCAACTGCTCTGAAGGCTACATTC
  AGAACTACAGATGCAGAGGTGATGACAGCAAAGTCCAGGAAGCCAGGAAGTCCTTCTTCT
  CTGGCCATGCCTCCTTCTCCATGTACACTATGCTGTATTTGGTGCTATACCTGCAGGCCC
  GCTTCACTTGGCGAGGAGCCCGCCTGCTCCGGCCCCTCCTGCAGTTCACCTTGATCATGA
  TGGCCTTCTACACGGGACTGTCTCGCGTATCAGACCACAAGCACCATCCCAGTGATGTTC
  TGGCAGGATTTGCTCAAGGAGCCCTGGTGGCCTGCTGCATAGTTTTCTTCGTGTCTGACC
  TCTTCAAGACTAAGATGACGCTCTCCCTGCCTGCCCCTGCTATCCGGAAGGAAATCCTTT
  CACCTGTGGACATTATTGACAGGAACAATCACCACAACATGATGTAGGTGCCACCCACCT
  CCTGAGCTGTTTTTGTAAAATGACTGCTGACAGCAAGTTCTTGCTGCTCTCCAATCTCAT
  CAGACAGTAGAATGTAGGGAAAAACTTTTGCCCGACTGATTTTTAAAAAAAAAAAAAAAA
  AA
• Restriction Sites: Please inquire
• ACCN: NM_003713
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003713.3, NP_003704.3
• RefSeq Size: 3310 bp
• RefSeq ORF: 936 bp
• Locus ID: 8613
• UniProt ID: O14495
• Cytogenetics: 1p32.2
• Domains: acidPPc
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Sphingolipid metabolism
• Gene Summary: The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is a membrane glycoprotein localized at the cell plasma membrane. It has been shown to actively hydrolyze extracellular lysophosphatidic acid and short-chain phosphatidic acid. The expression of this gene is found to be enhanced by epidermal growth factor in Hela cells. [provided by RefSeq, Mar 2010]