Phosphatidic acid phosphatase type 2B (PLPP3) (NM_003713) Human Tagged ORF Clone

CAT#: RG203480

  • TrueORF®

PPAP2B (tGFP-tagged) - Human phosphatidic acid phosphatase type 2B (PPAP2B)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK


  "NM_003713" in other vectors (7)

Reconstitution Protocol

USD 500.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Mouse monoclonal turboGFP antibody, clone OTI2H8
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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PPAP2B Rabbit polyclonal Antibody
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Other products for "Phosphatidic acid phosphatase type 2B"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol Phosphatidic acid phosphatase type 2B
Synonyms Dri42; LPP3; PAP2B; PPAP2B; VCIP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG203480 representing NM_003713
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAAACTACAAGTACGACAAAGCGATCGTCCCGGAGAGCAAGAACGGCGGCAGCCCGGCGCTCAACA
ACAACCCGAGGAGGAGCGGCAGCAAGCGGGTGCTGCTCATCTGCCTCGACCTCTTCTGCCTCTTCATGGC
GGGCCTCCCCTTCCTCATCATCGAGACAAGCACCATCAAGCCTTACCACCGAGGGTTTTACTGCAATGAT
GAGAGCATCAAGTACCCACTGAAAACTGGTGAGACAATAAATGACGCTGTGCTCTGTGCCGTGGGGATCG
TCATTGCCATCCTCGCGATCATCACGGGGGAATTCTACCGGATCTATTACCTGAAGAAGTCGCGGTCGAC
GATTCAGAACCCCTACGTGGCAGCACTCTATAAGCAAGTGGGCTGCTTCCTCTTTGGCTGTGCCATCAGC
CAGTCTTTCACAGACATTGCCAAAGTGTCCATAGGGCGCCTGCGTCCTCACTTCTTGAGTGTCTGCAACC
CTGATTTCAGCCAGATCAACTGCTCTGAAGGCTACATTCAGAACTACAGATGCAGAGGTGATGACAGCAA
AGTCCAGGAAGCCAGGAAGTCCTTCTTCTCTGGCCATGCCTCCTTCTCCATGTACACTATGCTGTATTTG
GTGCTATACCTGCAGGCCCGCTTCACTTGGCGAGGAGCCCGCCTGCTCCGGCCCCTCCTGCAGTTCACCT
TGATCATGATGGCCTTCTACACGGGACTGTCTCGCGTATCAGACCACAAGCACCATCCCAGTGATGTTCT
GGCAGGATTTGCTCAAGGAGCCCTGGTGGCCTGCTGCATAGTTTTCTTCGTGTCTGACCTCTTCAAGACT
AAGATGACGCTCTCCCTGCCTGCCCCTGCTATCCGGAAGGAAATCCTTTCACCTGTGGACATTATTGACA
GGAACAATCACCACAACATGATG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG203480 representing NM_003713
Red=Cloning site Green=Tags(s)

MQNYKYDKAIVPESKNGGSPALNNNPRRSGSKRVLLICLDLFCLFMAGLPFLIIETSTIKPYHRGFYCND
ESIKYPLKTGETINDAVLCAVGIVIAILAIITGEFYRIYYLKKSRSTIQNPYVAALYKQVGCFLFGCAIS
QSFTDIAKVSIGRLRPHFLSVCNPDFSQINCSEGYIQNYRCRGDDSKVQEARKSFFSGHASFSMYTMLYL
VLYLQARFTWRGARLLRPLLQFTLIMMAFYTGLSRVSDHKHHPSDVLAGFAQGALVACCIVFFVSDLFKT
KMTLSLPAPAIRKEILSPVDIIDRNNHHNMM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003713
ORF Size 933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003713.2
RefSeq Size 3310 bp
RefSeq ORF 936 bp
Locus ID 8613
UniProt ID O14495
Cytogenetics 1p32.2
Domains acidPPc
Protein Families Druggable Genome, Transmembrane
Protein Pathways Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Sphingolipid metabolism
Gene Summary The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is a membrane glycoprotein localized at the cell plasma membrane. It has been shown to actively hydrolyze extracellular lysophosphatidic acid and short-chain phosphatidic acid. The expression of this gene is found to be enhanced by epidermal growth factor in Hela cells. [provided by RefSeq, Mar 2010]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.