Phosphatidic acid phosphatase type 2B (PLPP3) (NM_003713) Human Untagged Clone

CAT#: SC117784

PPAP2B (untagged)-Human phosphatidic acid phosphatase type 2B (PPAP2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Phosphatidic acid phosphatase type 2B
• Synonyms: Dri42; LPP3; PAP2B; PPAP2B; VCIP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003713 edited
  GAATTCGGCACGAGGGGAAGGTGGCCGAGCGCCCGCGCTGCCCACTCGCTCGCTCGCGCA
  CTCAGACGCGCGCCACAACAGCGCGCCCCAAGCTGCGCAGCTCTGCAAAAGTTTCTGCTC
  GGGATCTGGCTCTCTTCCCCTTGGACTTTAGAACGATTTAGGGTTGACAGAGGAAAGCAG
  AGGCGCGCAGGAGGAGCAGAAAACACCACCTTCTGCAGTTGGAGGCAGGCAGCCCCGGCT
  GCACTCTAGCCGCCGCGCCCGGAGCCGGGGCCGACCCGCCACTATCCGCAGCAGCCTCGG
  CCAGGAGGCGACCCGGGCGCCTGGGTGTGTGGCTGCTGTTGCGGGACGTCTTCGCGGGGC
  GGGAGGCTCGCGCCGCAGCCAGCGCCATGCAAAACTACAAGTACGACAAAGCGATCGTCC
  CGGAGAGCAAGAACGGCGGCAGCCCGGCGCTCAACAACAACCCGAGGAGGAGCGGCAGCA
  AGCGGGTGCTGCTCATCTGCCTCGACCTCTTCTGCCTCTTCATGGCGGGCCTCCCCTTCC
  TCATCATCGAGACAAGCACCATCAAGCCTTACCACCGAGGGTTTTACTGCAATGATGAGA
  GCATCAAGTACCCACTGAAAACTGGTGAGACAATAAATGACGCTGTGCTCTGTGCCGTGG
  GGATCGTCATTGCCATCCTCGCGATCATCACGGGGGAATTCTACCGGATCTATTACCTGA
  AGAAGTCGCGGTCGACGATTCAGAACCCCTACGTGGCAGCACTCTATAAGCAAGTGGGCT
  GCTTCCTCTTTGGCTGTGCCATCAGCCAGTCTTTCACAGACATTGCCAAAGTGTCCATAG
  GGCGCCTGCGTCCTCACTTCTTGAGTGTCTGCAACCCTGATTTCAGCCAGATCAACTGCT
  CTGAAGGCTACATTCAGAACTACAGATGCAGAGGTGATGACAGCAAAGTCCAGGAAGCCA
  GGAAGTCCTTCTTCTCTGGCCATGCCTCCTTCTCCATGTACACTATGCTGTATTTGGTGC
  TATACCTGCAGGCCCGCTTCACTTGGCGAGGAGCCCGCCTGCTCCGGCCCCTCCTGCAGT
  TCACCTTGATCATGATGGCCTTCTACACGGGACTGTCTCGCGTATCAGACCACAAGCACC
  ATCCCAGTGATGTTCTGGCAGGATTTGCTCAAGGAGCCCTGGTGGCCTGCTGCATAGTTT
  TCTTCGTGTCTGACCTCTTCAAGACTAAGACGACGCTCTCCCTGCCTGCCCCTGCTATCC
  GGAAGGAAATCCTTTCACCTGTGGACATTATTGACAGGAACAATCACCACAACATGATGT
  AGGTGCCACCCACCTCCTGAGCTGTTTTTGTAAAATGACTGCTGACAGCAAGTTCTTGCT
  GCTCTCCAATCTCATCAGACAGTAGAATGTAGGGAAAAACTTTTGCCCGACTGATTTTTA
  AAAAGGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003713 unedited
  GGGCACCATTTGTAAACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGGAAGGTGG
  CCGAGCGCCCGCGCTGCCCACTCGCTCGCTCGCGCACTCAGACGCGCGCCACAACAGCGC
  GCCCCAAGCTGCGCAGCTCTGCAAAAGTTTCTGCTCGGGATCTGGCTCTCTTCCCCTTGG
  ACTTTAGAACGATTTAGGGTTGACAGAGGAAAGCAGAGGCGCGCAGGAGGAGCAGAAAAC
  ACCACCTTCTGCAGTTGGAGGCAGGCAGCCCCGGCTGCACTCTAGCCGCCGCGCCCGGAG
  CCGGGGCCGACCCGCCACTATCCGCAGCAGCCTCGGCCAGGAGGCGACCCGGGCGCCTGG
  GTGTGTGGCTGCTGTTGCGGGACGTCTTCGCGGGGCGGGAGGCTCGCGCCGCAGCCAGCG
  CCATGCAAAACTACAAGTACGACAAAGCGATCGTCCCGGAGAGCAAGAACGGCGGCAGCC
  CGGCGCTCAACAACAACCCGAGGAGGAGCGGCAGCAAGCGGGTGCTGCTCATCTGCCTCG
  ACCTCTTCTGCCTCTTCATGGCGGGCCTCCCCTTCCTCATCATCGAGACAAGCACCATCA
  AGCCTTACCACCGAGGGTTTTACTGCAATGATGAGAGCATCAAGTACCCACTGAAAACTG
  GTGAGACAATAAATGACGCTGTGCTCTGTGCCGTGGGGATCGTCATTGCCATCCTCGCGA
  TCATCACGGGGGAATTCTACCGGATCTATTACCTGAAGAAGTCGCGGTCGACGATTCAGA
  ACCCCNTACGTGCAGCACTCTATAAGCAGTGGGCTGCTTCCTCTNTGGCTGTGCCATCAG
  CCAGTCTTTCACAGACATTGCCAAGTGTCCATAGGGCGCCTGCGTNCTCACTTNCTGAGT
  GTCTGCACCCCTGATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003713 unedited
  GCGGCACGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTTTAAAAATCAGTCG
  GGCAAAAGTTTTTCCCTACATTCTACTGTCTGATGAGATTGGAGAGCAGCAAGAACTTGC
  TGTCAGCAGTCATTTTACAAAAACAGCTCAGGAGGTGGGTGGCACCTACATCATGTTGTG
  GTGATTGTTCCTGTCAATAATGTCCACAGGTGAAAGGATTTCCTTCCGGATAGCAGGGGC
  AGGCAGGGAGAGCGTCGTCTTATTCTTGAAGAGGTCAGACACGAAGAAAACTATGCAGCA
  GGCCACCAGGGCTCCTTGAGCAAATCCTGCCAAAACATCACTGGGATGGTGCTTGTGGTC
  TGATACGCGAGACAGTCCCGTGTAGAAGGCCATCATGATCAAGGTGAACTGCAGGAGGGG
  CCGGAGCAGGCGGGCTCCTCGCCAAGTGAAGCGGGCCTGCAGGTATAGCACCAAATACAG
  CATAGTGTACATGGAGAAGGAGGCATGGCCAGAGAAGAAGGACTTCCTGGCTTCCTGGAC
  TTTGCTGTCATCACCTCTGCATCTGTAGTTCTGAATGTAGCCTTCAGAGCAGTTGATCTG
  GCTGAAATCAGGGTTGCAGACACTCAAGAAGTGAGGACGCAGGCGCCCTATGGACACTTT
  GGCCATGTCTGTGAAAGACTGGCTGATGGCACACCCAAAAGGAAGCAGCCCACTTGCTTA
  TAGAGTGCTGCCACGTAGGGGTTTCTGAATCGTCGACCGCGACTTTTTCAGTAATAGATC
  CCGTAAATTTCCCCGTGATGATCGCCAAGATGCAATGACGATCCCCCCGCACATAGCCCA
  CGGTCTTTTTGGCTCACCGTTTTCATGGGTCCTTGTGCTCCATCATTGAAGAAAACCCTC
  GCGGAAAGCTCTATGTGCTTTTCTCAATATGAGAAAGGAACGCCCCCCTAAAAGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003713
• Insert Size: 1520 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003713.3, NP_003704.3
• RefSeq Size: 3310 bp
• RefSeq ORF: 936 bp
• Locus ID: 8613
• UniProt ID: O14495
• Cytogenetics: 1p32.2
• Domains: acidPPc
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Sphingolipid metabolism
• Gene Summary: The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is a membrane glycoprotein localized at the cell plasma membrane. It has been shown to actively hydrolyze extracellular lysophosphatidic acid and short-chain phosphatidic acid. The expression of this gene is found to be enhanced by epidermal growth factor in Hela cells. [provided by RefSeq, Mar 2010]