METTL14 (NM_020961) Human Untagged Clone

CAT#: SC319510

METTL14 (untagged)-Human methyltransferase like 14 (METTL14)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: METTL14
• Synonyms: hMETTL14
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020961.2 ATACTCTGTTCGTAAGCTCCCGGTGAATTTTGTTCCACAGACTCGGAAGAAAGGTTGGAT
  AAGAGTTCACTGGAGATTGACAAGTACTCGGGATAGTGAAAAGCCGGAGTTGGAACATGG
  ATAGCCGCTTGCAGGAGATCCGGGAGCGGCAGAAGTTACGGCGACAGCTCCTCGCGCAGC
  AGTTGGGAGCTGAAAGTGCCGACAGCATTGGTGCCGTGTTAAATAGCAAAGATGAGCAGA
  GAGAAATTGCTGAAACAAGAGAAACTTGCAGGGCTTCCTATGATACCTCTGCTCCAAATG
  CAAAACGTAAGTATCTGGATGAAGGAGAGACAGATGAAGACAAAATGGAAGAATATAAGG
  ATGAACTAGAAATGCAACAGGATGAAGAAAATTTGCCATATGAAGAAGAGATTTACAAAG
  ATTCTAGTACTTTTCTTAAGGGAACACAGAGCTTAAATCCCCATAATGATTACTGCCAAC
  ATTTTGTAGACACTGGACATAGACCTCAGAATTTCATCAGGGATGTAGGTTTAGCTGACA
  GATTTGAAGAATATCCTAAACTGAGGGAGCTCATCAGGCTAAAGGATGAGTTAATAGCTA
  AATCTAACACTCCTCCCATGTACTTACAAGCCGATATAGAAGCCTTTGACATCAGAGAAC
  TAACACCCAAATTTGATGTGATTCTTCTGGAACCCCCTTTAGAAGAATATTACAGAGAAA
  CTGGCATCACTGCTAATGAAAAATGCTGGACTTGGGATGATATTATGAAGTTAGAAATTG
  ATGAGATTGCAGCACCTCGATCATTTATTTTTCTCTGGTGTGGTTCTGGGGAGGGGTTGG
  ACCTTGGAAGAGTGTGTTTACGAAAATGGGGTTACAGAAGATGTGAAGATATTTGTTGGA
  TTAAAACCAATAAAAACAATCCTGGGAAGACTAAGACTTTAGATCCAAAGGCTGTCTTTC
  AGAGAACAAAGGAACACTGCCTCATGGGGATCAAAGGAACTGTGAAGCGTAGCACAGACG
  GGGACTTCATTCATGCTAATGTTGACATTGACTTAATTATCACAGAAGAACCTGAAATTG
  GCAATATAGAAAAACCTGTAGAAATTTTTCATATAATTGAGCATTTTTGTCTTGGTAGAA
  GACGCCTTCATCTATTTGGAAGAGATAGTACAATTCGACCAGGCTGGCTCACAGTTGGAC
  CAACGCTTACAAATAGCAACTACAATGCAGAAACATATGCATCCTATTTCAGTGCTCCTA
  ATTCCTACTTGACTGGTTGTACAGAAGAAATTGAGAGACTTCGACCAAAATCGCCTCCTC
  CCAAATCTAAATCTGACCGAGGAGGTGGAGCTCCCAGAGGTGGAGGAAGAGGTGGAACTT
  CTGCTGGCCGTGGACGAGAAAGAAATAGATCTAACTTCCGAGGAGAAAGAGGTGGCTTTA
  GAGGGGGCCGTGGAGGAGCACACAGAGGTGGCTTTCCACCTCGATAATTGTTGAAGACAT
  TGAACCTATTCATCCTCCTCTAACCTTCTTTATTGTAATTAAATTTCAAGTGGGAGACTT
  AACTTTAGAACTCACTTCCAGCTTGCACTTTGCTTTAATTTCTCTGAGCTGCAAGAATGT
  CTTAGCGAGCCTTGCTTGCAGTTGTCACACACACTGTCCGGTTTTTTTCAGGATAAATGA
  ATGATTCTGCCTTTTGTTATGTGCGTGAACAGAATGGAACAACTCAAGTAGCTTCATCTT
  CAGAGACTGAATTTATTCTGATAGACTTCAGCTAATTACAAAGGATTTTGCTAATTTTTG
  GGAATAAATAATGGAAAAAGATCCAGTCTGTGGTATCATGCTAGTGCTGACAGGGCCTTG
  ATAGAATAGAGTTGGAAAAGATGGTAAGCTTTTGTCAGGGTTTTAACATTTTCTTGATGA
  AACAATAAAAAGAGGTAAGCTTTTTTCTTCTTTTTTTTTAAGTTTTAAATAAACTCAGAT
  ATAATTTGAATACTGAAGAAATTAAGAGACTTTGAACAAAATCTCTTCCCAAATCTAAAT
  TTGATAGGGGAGGTGGAGATTCCAGGGGTGGGTGAAAGAAGAGATAGAACTTAGCAGGCA
  GACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_020961
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020961.2, NP_066012.1
• RefSeq Size: 2138 bp
• RefSeq ORF: 1371 bp
• Locus ID: 57721
• UniProt ID: Q9HCE5
• Cytogenetics: 4q26
• Domains: MT-A70
• Gene Summary: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:29348140, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194, PubMed:29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).[UniProtKB/Swiss-Prot Function]