DCP1A (NM_018403) Human Untagged Clone

CAT#: SC319488

DCP1A (untagged)-Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCP1A
• Synonyms: HSA275986; Nbla00360; SMAD4IP1; SMIF
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018403.3 GATGGAGGCGCTGAGTCGAGCTGGGCAGGAGATGAGCCTAGCGGCCCTGAAGCAACACGA
  CCCCTATATCACCAGCATCGCAGACCTCACGGGCCAGGTCGCTCTGTACACCTTCTGCCC
  CAAGGCCAACCAGTGGGAGAAGACTGATATAGAAGGGACCTTATTCGTATATCGAAGGTC
  AGCTTCCCCTTACCATGGTTTTACCATTGTGAATCGACTAAATATGCACAATCTAGTTGA
  ACCAGTGAATAAAGATTTGGAATTTCAGCTCCATGAACCATTTCTTCTGTATAGAAATGC
  AAGCTTGTCGATATATAGTATCTGGTTTTATGACAAGAATGACTGTCACCGCATAGCAAA
  ACTCATGGCTGATGTGGTAGAAGAGGAGACACGGCGATCCCAGCAAGCTGCTCGGGACAA
  ACAGAGTCCCAGCCAGGCCAATGGCTGCAGCGACCACAGGCCCATCGACATCCTGGAGAT
  GCTGAGCAGAGCCAAGGATGAGTATGAGAGGAATCAGATGGGTGACTCAAATATCTCCAG
  CCCTGGGTTACAGCCAAGCACTCAGCTCTCCAATCTGGGAAGCACCGAGACTCTAGAAGA
  AATGCCCTCCGGGTCACAGGATAAGTCTGCTCCATCTGGACACAAGCATCTGACGGTAGA
  AGAGTTATTTGGAACCTCTTTGCCAAAGGAACAACCAGCAGTTGTGGGTCTGGATTCAGA
  AGAAATGGAGAGGTTGCCAGGAGATGCCTCCCAGAAAGAGCCCAATTCATTCCTACCATT
  TCCCTTTGAGCAGTTAGGAGGAGCCCCTCAATCAGAAACCCTGGGTGTCCCTTCTGCTGC
  CCACCATTCAGTCCAGCCTGAAATCACCACCCCGGTGCTAATCACTCCAGCCTCCATCAC
  ACAGTCCAATGAAAAGCATGCTCCAACCTACACAATCCCGTTGAGCCCTGTTCTCAGTCC
  CACTCTGCCAGCTGAAGCTCCTACTGCACAGGTTCCCCCCAGCTTACCTCGAAACAGCAC
  CATGATGCAGGCAGTGAAGACCACGCCTAGACAGAGGTCTCCACTCCTGAACCAGCCAGT
  CCCTGAGCTAAGCCATGCCAGTCTGATTGCCAACCAGAGCCCCTTCAGGGCCCCATTGAA
  CGTGACGAACACAGCTGGCACATCCCTCCCAAGCGTTGATCTTCTCCAGAAACTCAGGTT
  GACCCCACAGCATGACCAAATACAGACACAACCACTTGGGAAAGGTGCAATGGTAGCCAG
  CTTTTCTCCGGCAGCTGGTCAGCTAGCCACACCTGAGAGCTTCATAGAGCCTCCCTCTAA
  GACAGCAGCAGCAAGAGTGGCGGCCTCAGCCTCCCTGAGCAACATGGTGCTTGCTCCCCT
  TCAGTCTATGCAGCAGAACCAGGATCCTGAAGTATTTGTGCAGCCTAAGGTGTTATCCAG
  TGCCATCCCGGTTGCAGGCGCCCCACTGGTTACTGCAACGACCACTGCAGTGTCTTCAGT
  CCTGCTGGCCCCAAGTGTTTTCCAGCAGACAGTTACAAGATCTTCGGACCTTGAGAGGAA
  AGCCAGCTCCCCTTCTCCTCTAACTATTGGAACGCCAGAAAGTCAGAGAAAGCCTTCCAT
  TATTCTCAGCAAGTCTCAGCTCCAGGATACATTAATACATCTAATAAAGAATGATTCCAG
  CTTCCTCAGTACACTTCATGAAGTCTACTTGCAGGTTCTGACCAAGAACAAAGACAACCA
  CAACCTATGACTGGAGCAGAATAAGTCTAAAGGCAGAGCCCCAGCCTCAGAGACAAATAG
  GCTTCATCATGGAAACTTCTGAGCAGAACATTGAGTTTGAGAATCCTGAAATTGAGCCTA
  TGAGAAAGAGACTCATTTCTTAAGAATGTTTTCCAAGTGACATTCTCAGTGATAATGGAG
  GTTTCACTGTGAAGCATCACCAGCAGATCCTTGTTTTGACAAAAAAAAAAAAAAAAAAAA
  AA
• Restriction Sites: Please inquire
• ACCN: NM_018403
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018403.3, NP_060873.3
• RefSeq Size: 2054 bp
• RefSeq ORF: 1749 bp
• Locus ID: 55802
• UniProt ID: Q9NPI6
• Cytogenetics: 3p21.1
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.