B3GAT3 (NM_012200) Human Untagged Clone

CAT#: SC319206

B3GAT3 (untagged)-Human beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (B3GAT3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B3GAT3
• Synonyms: GLCATI; glcUAT-I; JDSCD
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012200.2 CTGCTCGGGCGCGGGCGGCGGCGCGGCCATGAAGCTGAAGCTGAAGAACGTGTTTCTCGC
  CTACTTCCTGGTGTCGATCGCCGGCCTCCTCTACGCGCTGGTACAGCTCGGCCAGCCATG
  TGACTGCCTTCCTCCCCTGCGGGCAGCAGCCGAGCAGCTACGGCAGAAGGATCTGAGGAT
  TTCCCAGCTGCAAGCGGAACTCCGACGGCCACCCCCTGCCCCTGCCCAGCCCCCTGAACC
  CGAGGCCCTGCCTACTATCTATGTTGTTACCCCCACCTATGCCAGGCTGGTACAGAAGGC
  AGAGCTGGTACGACTGTCCCAGACACTGAGCCTGGTGCCCCGGCTGCATTGGCTGCTGGT
  GGAGGATGCTGAGGGTCCCACCCCGCTGGTCTCAGGGCTGCTGGCTGCCTCTGGCCTCCT
  CTTCACACACCTGGTGGTCCTCACGCCCAAAGCCCAGCGGCTTCGGGAGGGCGAGCCTGG
  CTGGGTTCATCCCCGTGGTGTCGAGCAGCGGAACAAGGCCCTGGACTGGCTCCGGGGCAG
  AGGGGGTGCTGTGGGTGGGGAGAAGGACCCACCACCACCAGGGACCCAAGGAGTCGTCTA
  CTTTGCTGACGATGACAACACCTACAGCCGGGAGCTGTTTGAGGAGATGCGCTGGACCCG
  TGGTGTCTCAGTGTGGCCTGTGGGGCTGGTGGGCGGCCTGCGATTCGAGGGCCCTCAGGT
  ACAGGACGGCCGGGTAGTGGGCTTCCACACAGCATGGGAGCCCAGCAGGCCCTTCCCTGT
  GGATATGGCTGGATTTGCCGTGGCCCTGCCCTTGCTGTTAGATAAGCCCAATGCCCAATT
  TGATTCCACCGCTCCCCGGGGCCACCTGGAGAGCAGTCTTCTGAGCCACCTTGTGGATCC
  CAAGGACCTGGAGCCACGGGCTGCCAACTGCACTCGGGTACTGGTGTGGCATACTCGGAC
  AGAGAAGCCCAAGATGAAGCAGGAGGAGCAGCTGCAGCGGCAGGGCCGGGGCTCAGACCC
  AGCAATTGAGGTGTGATGGCGGCCCCACCCCAACTACCACCTCTTTTCAGGCACAGACCT
  TGTGGGACTGGGCCCCAGGCCTGCCCAGGATGTGGTTTTCCAAGTCCTGACCCTTGGAGC
  CAGAAGTGGCCCCTCTGCCCCTCCAGGCCCAGGGCATGGTCCTGCTGCTTCACCCCTCCC
  CTAGCCTGCCGTGTGGCACTGCCCACAGGCTGGGGACAAGCAGCCCTTGTGTTGAGTCAG
  GTTGGCCCTGTCTAGGGTGGAACAGAAGGACAGATGGACCCAGGAGGGAGGGCAGCTGAG
  TAACTGGGTAACTTATTGGGGCTGGGCATGCACTGGGGGGCTGGAGGAGCTGGGCTGGAC
  CCTTCCCACCTGAGCATGCTGACCCCCTTCCTACCTCCAGAATAAAGAATCTCAACCTGG
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_012200
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012200.2, NP_036332.2
• RefSeq Size: 1456 bp
• RefSeq ORF: 1008 bp
• Locus ID: 26229
• UniProt ID: O94766
• Cytogenetics: 11q12.3
• Domains: Glyco_transf_43
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a member of the glucuronyltransferase gene family, enzymes that exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product catalyzes the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans. A pseudogene of this gene has been identified on chromosome 3. [provided by RefSeq, Dec 2013]
  Transcript Variant: This variant (1) encodes the longest isoform (1).