NOX5 (NM_024505) Human Untagged Clone

CAT#: SC318228

NOX5 (untagged)-Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NOX5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318228 representing NM_024505.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAACACATCTGGAGACCCAGCCCAGACGGGCCCTGAAGGCTGTAGAGGCACCATGAGTGCCGAGGAG
  GATGCCAGGTGGCTCCGGTGGGTGACTCAGCAGTTTAAGACCATTGCAGGAGAAGATGGGGAGATCAGC
  CTGCAAGAATTCAAAGCAGCTCTGCATGTGAAAGAGTCCTTCTTTGCAGAGCGATTCTTTGCCCTATTT
  GACTCCGATAGAAGTGGCACCATCACCCTCCAGGAGCTGCAGGAGGCACTGACCCTGCTCATCCATGGC
  AGCCCCATGGACAAACTCAAATTCCTCTTCCAGGTGTATGACATCGATGTGTGTGCACGGCAGGGGGCG
  TCTGCAGGTACAGAGTGGGGTGCTGGGGCAGGCCCGCACTGGGCTTCATCCCCACTCGGGACAGGCAGT
  GGCTCCATTGACCCGGATGAGCTGCGCACTGTGCTGCAGTCGTGTCTGCGCGAGAGCGCCATCTCGCTG
  CCTGACGAGAAGCTGGACCAGCTGACGCTGGCGCTCTTCGAATCGGCCGACGCGGACGGCAACGGGGCC
  ATCACCTTCGAGGAGCTCCGGGACGAGCTGCAGCGCTTCCCCGGAGTCATGGAGAACCTGACCATCAGC
  GCTGCCCACTGGCTGACGGCCCCCGCCCCCCGCCCACGCCCGCGCCGGCCGCGCCAGCTGACCCGCGCC
  TACTGGCACAACCACCGCAGCCAGCTGTTCTGCCTGGCCACCTATGCAGGCCTCCACGTGCTGCTCTTC
  GGGCTGGCGGCCAGCGCGCACCGGGACCTCGGCGCCAGCGTCATGGTGGCCAAGGGCTGCGGCCAGTGC
  CTCAACTTCGACTGCAGCTTCATCGCGGTGCTGATGCTCAGACGCTGCCTCACCTGGCTGCGGGCCACG
  TGGCTGGCTCAAGTCCTACCACTGGACCAGAACATCCAGTTCCACCAGCTTATGGGCTACGTGGTAGTG
  GGGCTGTCCCTCGTGCACACCGTGGCTCACACTGTGAACTTTGTACTCCAGGCTCAGGCGGAGGCCAGC
  CCTTTCCAGTTCTGGGAGCTGCTGCTCACCACGAGGCCTGGCATTGGCTGGGTACACGGTTCGGCCTCC
  CCGACAGGTGTCGCTCTGCTGCTGCTGCTCCTCCTCATGTTCATCTGCTCCAGTTCCTGCATCCGCAGG
  AGTGGCCACTTTGAGGTGTTCTATTGGACTCACCTGTCCTACCTCCTCGTGTGGCTTCTGCTCATCTTT
  CATGGGCCCAACTTCTGGAAGTGGCTGCTGGTGCCTGGAATCTTGTTTTTCCTGGAGAAGGCCATCGGA
  CTGGCAGTGTCCCGCATGGCAGCCGTGTGCATCATGGAAGTCAACCTCCTCCCCTCCAAGGTCACTCAT
  CTCCTCATCAAGCGGCCCCCTTTTTTTCACTATAGACCTGGTGACTACTTGTATCTGAACATCCCCACC
  ATTGCTCGCTATGAGTGGCACCCCTTCACCATCAGCAGTGCTCCTGAGCAGAAAGACACTATCTGGCTG
  CACATTCGGTCCCAAGGCCAGTGGACAAACAGGCTGTATGAGTCCTTCAAGGCATCAGACCCACTGGGC
  CGTGGTTCTAAGAGGCTGTCGAGGAGTGTGACAATGAGAAAGAGTCAAAGGTCGTCCAAGGGCTCTGAG
  ATACTTTTGGAGAAACACAAATTCTGTAACATCAAGTGCTACATCGATGGGCCTTATGGGACCCCCACC
  CGCAGGATCTTTGCCTCTGAGCATGCCGTGCTCATCGGGGCAGGCATCGGCATCACCCCCTTTGCTTCC
  ATTCTGCAGAGTATCATGTACAGGCACCAGAAAAGAAAGCATACTTGCCCCAGCTGCCAGCACTCCTGG
  ATCGAAGGTGTCCAAGACAACATGAAGCTCCATAAGGTGGACTTTATCTGGATCAACAGAGACCAGCGG
  TCTTTCGAGTGGTTTGTGAGCCTGCTGACTAAACTGGAGATGGACCAGGCCGAGGAGGCTCAATACGGC
  CGCTTCCTGGAGCTGCATATGTACATGACATCTGCACTGGGCAAGAATGACATGAAGGCCATTGGCCTG
  CAGATGGCCCTTGACCTCCTGGCCAACAAGGAGAAGAAAGACTCCATCACGGGGCTGCAGACGCGCACC
  CAGCCTGGGCGGCCTGACTGGAGCAAGGTGTTCCAGAAAGTGGCTGCTGAGAAGAAGGGCAAGGTGCAG
  GTCTTCTTCTGTGGCTCCCCAGCTCTGGCCAAGGTGCTGAAGGGCCATTGTGAGAAGTTCGGCTTCAGA
  TTTTTCCAAGAGAATTTCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_024505
• Insert Size: 2298 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024505.3
• RefSeq Size: 2809 bp
• RefSeq ORF: 2298 bp
• Locus ID: 79400
• UniProt ID: Q96PH1
• Cytogenetics: 15q23
• Protein Families: Ion Channels: Other, Transmembrane
• MW: 86.4 kDa
• Gene Summary: This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1, alternately referred to as NOX5 gamma and v3) encodes the longest isoform (1).