NOX5 (NM_024505) Human Untagged Clone

CAT#: SC123954

NOX5 (untagged)-Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NOX5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024505, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACACATCTGGAGACCCAGCCCAGACGGGCCCTGAAGGCTGTAGAGGCACCATGAGTGCCGAGGAGG
  ATGCCAGGTGGCTCCGGTGGGTGACTCAGCAGTTTAAGACCATTGCAGGAGAAGATGGGGAGATCAGCCT
  GCAAGAATTCAAAGCAGCTCTGCATGTGAAAGAGTCCTTCTTTGCAGAGCGATTCTTTGCCCTATTTGAC
  TCCGATAGAAGTGGCACCATCACCCTCCAGGAGCTGCAGGAGGCACTGACCCTGCTCATCCATGGCAGCC
  CCATGGACAAACTCAAATTCCTCTTCCAGGTGTATGACATCGATGTGTGTGCACGGCAGGGGGCGTCTGC
  AGGTACAGAGTGGGGTGCTGGGGCAGGCCCGCACTGGGCTTCATCCCCACTCGGGACAGGCAGTGGCTCC
  ATTGACCCGGATGAGCTGCGCACTGTGCTGCAGTCGTGTCTGCGCGAGAGCGCCATCTCGCTGCCTGACG
  AGAAGCTGGACCAGCTGACGCTGGCGCTCTTCGAATCGGCCGACGCGGACGGCAACGGGGCCATCACCTT
  CGAGGAGCTCCGGGACGAGCTGCAGCGCTTCCCCGGAGTCATGGAGAACCTGACCATCAGCGCTGCCCAC
  TGGCTGACGGCCCCCGCCCCCCGCCCACGCCCGCGCCGGCCGCGCCAGCTGACCCGCGCCTACTGGCACA
  ACCACCGCAGCCAGCTGTTCTGCCTGGCCACCTATGCAGGCCTCCACGTGCTGCTCTTCGGGCTGGCGGC
  CAGCGCGCACCGGGACCTCGGCGCCAGCGTCATGGTGGCCAAGGGCTGCGGCCAGTGCCTCAACTTCGAC
  TGCAGCTTCATCGCGGTGCTGATGCTCAGACGCTGCCTCACCTGGCTGCGGGCCACGTGGCTGGCTCAAG
  TCCTACCACTGGACCAGAACATCCAGTTCCACCAGCTTATGGGCTACGTGGTAGTGGGGCTGTCCCTCGT
  GCACACCGTGGCTCACACTGTGAACTTTGTACTCCAGGCTCAGGCGGAGGCCAGCCCTTTCCAGTTCTGG
  GAGCTGCTGCTCACCACGAGGCCTGGCATTGGCTGGGTACACGGTTCGGCCTCCCCGACAGGTGTCGCTC
  TGCTGCTGCTGCTCCTCCTCATGTTCATCTGCTCCAGTTCCTGCATCCGCAGGAGTGGCCACTTTGAGGT
  GTTCTATTGGACTCACCTGTCCTACCTCCTCGTGTGGCTTCTGCTCATCTTTCATGGGCCCAACTTCTGG
  AAGTGGCTGCTGGTGCCTGGAATCTTGTTTTTCCTGGAGAAGGCCATCGGACTGGCAGTGTCCCGCATGG
  CAGCCGTGTGCATCATGGAAGTCAACCTCCTCCCCTCCAAGGTCACTCATCTCCTCATCAAGCGGCCCCC
  TTTTTTTCACTATAGACCTGGTGACTACTTGTATCTGAACATCCCCACCATTGCTCGCTATGAGTGGCAC
  CCCTTCACCATCAGCAGTGCTCCTGAGCAGAAAGACACTATCTGGCTGCACATTCGGTCCCAAGGCCAGT
  GGACAAACAGGCTGTATGAGTCCTTCAAGGCATCAGACCCACTGGGCCGTGGTTCTAAGAGGCTGTCGAG
  GAGTGTGACAATGAGAAAGAGTCAAAGGTCGTCCAAGGGCTCTGAGATACTTTTGGAGAAACACAAATTC
  TGTAACATCAAGTGCTACATCGATGGGCCTTATGGGACCCCCACCCGCAGGATCTTTGCCTCTGAGCATG
  CCGTGCTCATCGGGGCAGGCATCGGCATCACCCCCTTTGCTTCCATTCTGCAGAGTATCATGTACAGGCA
  CCAGAAAAGAAAGCATACTTGCCCCAGCTGCCAGCACTCCTGGATCGAAGGTGTCCAAGACAACATGAAG
  CTCCATAAGGTGGACTTTATCTGGATCAACAGAGACCAGCGGTCTTTCGAGTGGTTTGTGAGCCTGCTGA
  CTAAACTGGAGATGGACCAGGCCGAGGAGGCTCAATACGGCCGCTTCCTGGAGCTGCATATGTACATGAC
  ATCTGCACTGGGCAAGAATGACATGAAGGCCATTGGCCTGCAGATGGCCCTTGACCTCCTGGCCAACAAG
  GAGAAGAAAGACTCCATCACGGGGCTGCAGACGCGCACCCAGCCTGGGCGGCCTGACTGGAGCAAGGTGT
  TCCAGAAAGTGGCTGCTGAGAAGAAGGGCAAGGTGCAGGTCTTCTTCTGTGGCTCCCCAGCTCTGGCCAA
  GGTGCTGAAGGGCCATTGTGAGAAGTTCGGCTTCAGATTTTTCCAAGAGAATTTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024505 unedited
  NATTCAGCCCGCTATCGGGCAGGCATCGGCATCACCCCCTTTGCTTCCATTCTGCAGAGT
  ATCATGTACAGGCACCAGAAAAGAAAGCATACTTGCCCCAGCTGCCAGCACTCCTGGATC
  GAAGGTGTCCAAGACAACATGAAGCTCCATAAGGTGGACTTTATCTGGATCAACAGAGAC
  CAGCGGTCTTTCGAGTGGTTTGTGAGCCTGCTGACTAAACTGGAGATGGACCAGGCCGAG
  GAGGCTCAATACGGCCGCTTCCTGGAGCTGCATATGTACATGACATCTGCACTGGGCAAG
  AATGACATGAAGGCCATTGGCCTGCAGATGGCCCTTGACCTCCTGGCCAACAAGGAGAAG
  AAAGACTCCATCACGGGGCTGCAGACGCGCACCCAGCCTGGGCGGCCTGACTGGAGCAAG
  GTGTTCCAGAAAGTGGCTGCTGAGAAGAAGGGCAAGGTGCAGGTCTTCTTCTGTGGCTCC
  CCAGCTCTGGCCAAGGTGCTGAAGGGCCATTGTGAGAAGTTCGGCTTCAGATTTTTCCAA
  GAGAATTTCTAGCCTCACCTCTCCAAGCTCTGCCCCAAGTCCACACCATGGGTCTGCTTC
  ATCGCATTAGTATAAATGCCCCCACAGGGACCAGCCTCAGATGACCCACCCAATAAGAAC
  AAGCCTATGGACCCCCCTATCCTGCTCAACGAGAGGACAAGAGAACCCAAGGGCAAATGA
  ACTTCCCTAGACCCCNGGGAAGGGACANTGCTTGTTAATCTGCGGAGATTCTGGGTTCTT
  GAAATTAAGAACCCAAGTGGTCCGGGACTTGGGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024505 unedited
  NATACCCCGTTGCCGTCCGCGTCGGCCGATTCGAAGAGCGCCAGCGTCAGCTGGTCCAGC
  TTCTCGTCAGGCAGCGAGATGGCGCTCTCGCGCAGACACGACTGCAGCACAGTGCGCAGC
  TCATCCGGGTCAATGGAGCCACTGCCTGTCCCGAGTGGGGATGAAGCCCAGTGCGGGCCT
  GCCCCAGCACCCCACTCTGTACCTGCAGACGCCCCCTGCCGTGCACACACATCGATGTCA
  TACACCTGGAAGAGGAATTTGAGTTTGTCCATGGGGCTGCCATGGATGAGCAGGGTCAGT
  GCCTCCTGCAGCTCCTGGAGGGTGATGGTGCCACTTCTATCGGAGTCAAATAGGGCAAAG
  AATCGCTCTGCAAAGAAGGACTCTTTCACATGCAGAGCTGCTTTGAATTCTTGCAGGCTG
  ATCTCCCCATCTTCTCCTGCAATGGTCTTAAACTGCTGAGTCACCCACCGGAGCCACCTG
  GCATCCTCCTCGGCACTCATGGTGCCTCTACAGCCTTCAGGGCCCGTCTGGGCTGGGTCT
  CCAGCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGT
  TCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCG
  TCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTTCCGTTGATTTTGTTGCCAAACAA
  AACTCCCATGGCCTCATGGGGTTGAAACCTGGAATATCCTGTGTTAAAACTTTTCCGCCC
  CATTGGTGCTGCCAAACCCCTTCCCTGGTATAGCGTTATAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024505
• Insert Size: 3200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024505.1, NP_078781.1
• RefSeq Size: 2809 bp
• RefSeq ORF: 1698 bp
• Locus ID: 79400
• UniProt ID: Q96PH1
• Cytogenetics: 15q23
• Protein Families: Ion Channels: Other, Transmembrane
• Gene Summary: This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1, alternately referred to as NOX5 gamma and v3) encodes the longest isoform (1).