NOX5 (NM_024505) Human Tagged ORF Clone

CAT#: RG214207

NOX5 (tGFP-tagged) - Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 1

Product Images

Circular map for RG214207

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NOX5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214207 representing NM_024505
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCCGAGGAGGATGCCAGGTGGCTCCGGTGGGTGACTCAGCAGTTTAAGACCATTGCAGGAGAAG
  ATGGGGAGATCAGCCTGCAAGAATTCAAAGCAGCTCTGCATGTGAAAGAGTCCTTCTTTGCAGAGCGATT
  CTTTGCCCTATTTGACTCCGATAGAAGTGGCACCATCACCCTCCAGGAGCTGCAGGAGGCACTGACCCTG
  CTCATCCATGGCAGCCCCATGGACAAACTCAAATTCCTCTTCCAGGTGTATGACATCGATGTGTGTGCAC
  GGCAGGGGGCGTCTGCAGGTACAGAGTGGGGTGCTGGGGCAGGCCCGCACTGGGCTTCATCCCCACTCGG
  GACAGGCAGTGGCTCCATTGACCCGGATGAGCTGCGCACTGTGCTGCAGTCGTGTCTGCGCGAGAGCGCC
  ATCTCGCTGCCTGACGAGAAGCTGGACCAGCTGACGCTGGCGCTCTTCGAATCGGCCGACGCGGACGGCA
  ACGGGGCCATCACCTTCGAGGAGCTCCGGGACGAGCTGCAGCGCTTCCCCGGAGTCATGGAGAACCTGAC
  CATCAGCGCTGCCCACTGGCTGACGGCCCCCGCCCCCCGCCCACGCCCGCGCCGGCCGCGCCAGCTGACC
  CGCGCCTACTGGCACAACCACCGCAGCCAGCTGTTCTGCCTGGCCACCTATGCAGGCCTCCACGTGCTGC
  TCTTCGGGCTGGCGGCCAGCGCGCACCGGGACCTCGGCGCCAGCGTCATGGTGGCCAAGGGCTGTGGCCA
  GTGCCTCAACTTCGACTGCAGCTTCATCGCGGTGCTGATGCTCAGACGCTGCCTCACCTGGCTGCGGGCC
  ACGTGGCTGGCTCAAGTCCTACCACTGGACCAGAACATCCAGTTCCACCAGCTTATGGGCTACGTGGTAG
  TGGGGCTGTCCCTCGTGCACACTGTGGCTCACACTGTGAACTTTGTACTCCAGGCTCAGGCGGAGGCCAG
  CCCTTTCCAGTTCTGGGAGCTGCTGCTCACCACGAGGCCTGGCATTGGCTGGGTACACGGTTCGGCCTCC
  CCGACAGGTGTCGCTCTGCTGCTGCTGCTCCTCCTCATGTTCATCTGCTCCAGTTCCTGCATCCGCAGGA
  GTGGCCACTTTGAGGTGTTCTATTGGACTCACCTGTCCTACCTCCTCGTGTGGCTTCTGCTCATCTTTCA
  TGGGCCCAACTTCTGGAAGTGGCTGCTGGTGCCTGGAATCTTGTTTTTCCTGGAGAAGGCCATCGGACTG
  GCAGTGTCCCGCATGGCAGCCGTGTGCATCATGGAAGTCAACCTCCTCCCCTCCAAGGTCACTCATCTCC
  TCATCAAGCGGCCCCCTTTTTTTCACTATAGACCTGGTGACTACTTGTATCTGAACATCCCCACCATTGC
  TCGCTATGAGTGGCACCCCTTCACCATCAGCAGTGCTCCTGAGCAGAAAGACACTATCTGGCTGCACATT
  CGGTCCCAAGGCCAGTGGACAAACAGGCTGTATGAGTCCTTCAAGGCATCAGACCCACTGGGCCGTGGTT
  CTAAGAGGCTGTCGAGGAGTGTGACAATGAGAAAGAGTCAAAGGTCGTCCAAGGGCTCTGAGATACTTTT
  GGAGAAACACAAATTCTGTAACATCAAGTGCTACATCGATGGGCCTTATGGGACCCCCACCCGCAGGATC
  TTTGCCTCTGAGCATGCCGTGCTCATCGGGGCAGGCATCGGCATCACCCCCTTTGCTTCCATTCTGCAGA
  GTATCATGTACAGGCACCAGAAAAGAAAGCATACTTGCCCCAGCTGCCAGCACTCCTGGATCGAAGGTGT
  CCAAGACAACATGAAGCTCCATAAGGTGGACTTTATCTGGATCAACAGAGACCAGCGGTCTTTCGAGTGG
  TTTGTGAGCCTGCTGACTAAACTGGAGATGGACCAGGCCGAGGAGGCTCAATACGGCCGCTTCCTGGAGC
  TGCATATGTACATGACATCTGCACTGGGCAAGAATGACATGAAGGCCATTGGCCTGCAGATGGCCCTTGA
  CCTCCTGGCCAACAAGGAGAAGAAAGACTCCATCACGGGGCTGCAGACGCGCACCCAGCCTGGGCGGCCT
  GACTGGAGCAAGGTGTTCCAGAAAGTGGCTGCTGAGAAGAAGGGCAAGGTGCAGGTCTTCTTCTGTGGCT
  CCCCAGCTCTGGCCAAGGTGCTGAAGGGCCATTGTGAGAAGTTCGGCTTCAGATTTTTCCAAGAGAATTT
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214207 representing NM_024505
  Red=Cloning site Green=Tags(s)
  
  MSAEEDARWLRWVTQQFKTIAGEDGEISLQEFKAALHVKESFFAERFFALFDSDRSGTITLQELQEALTL
  LIHGSPMDKLKFLFQVYDIDVCARQGASAGTEWGAGAGPHWASSPLGTGSGSIDPDELRTVLQSCLRESA
  ISLPDEKLDQLTLALFESADADGNGAITFEELRDELQRFPGVMENLTISAAHWLTAPAPRPRPRRPRQLT
  RAYWHNHRSQLFCLATYAGLHVLLFGLAASAHRDLGASVMVAKGCGQCLNFDCSFIAVLMLRRCLTWLRA
  TWLAQVLPLDQNIQFHQLMGYVVVGLSLVHTVAHTVNFVLQAQAEASPFQFWELLLTTRPGIGWVHGSAS
  PTGVALLLLLLLMFICSSSCIRRSGHFEVFYWTHLSYLLVWLLLIFHGPNFWKWLLVPGILFFLEKAIGL
  AVSRMAAVCIMEVNLLPSKVTHLLIKRPPFFHYRPGDYLYLNIPTIARYEWHPFTISSAPEQKDTIWLHI
  RSQGQWTNRLYESFKASDPLGRGSKRLSRSVTMRKSQRSSKGSEILLEKHKFCNIKCYIDGPYGTPTRRI
  FASEHAVLIGAGIGITPFASILQSIMYRHQKRKHTCPSCQHSWIEGVQDNMKLHKVDFIWINRDQRSFEW
  FVSLLTKLEMDQAEEAQYGRFLELHMYMTSALGKNDMKAIGLQMALDLLANKEKKDSITGLQTRTQPGRP
  DWSKVFQKVAAEKKGKVQVFFCGSPALAKVLKGHCEKFGFRFFQENF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024505
• ORF Size: 2241 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024505.2, NP_078781.2
• RefSeq Size: 2582 bp
• RefSeq ORF: 2298 bp
• Locus ID: 79400
• UniProt ID: Q96PH1
• Cytogenetics: 15q23
• Protein Families: Ion Channels: Other, Transmembrane
• Gene Summary: This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]