MTMR14 (NM_022485) Human Untagged Clone

CAT#: SC317910

MTMR14 (untagged)-Human myotubularin related protein 14 (MTMR14), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTMR14
• Synonyms: C3orf29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317910 representing NM_022485.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCGGCGCTCGGGCCGCCGCCGCCGCTGCCTCGGCGGGGTCCTCGGCCTCTTCAGGCAACCAGCCG
  CCTCAGGAGCTGGGGCTTGGGGAGCTGCTGGAGGAGTTCTCCCGGACTCAGTACCGGGCCAAGGATGGC
  AGCGGGACCGGCGGCTCTAAGGTTGAGCGCATTGAGAAGAGATGTCTGGAGCTGTTTGGCCGAGACTAC
  TGTTTCAGCGTGATTCCAAACACGAATGGGGATATCTGTGGCCACTATCCCCGGCACATCGTGTTCCTG
  GAGTATGAGAGTTCTGAGAAGGAGAAAGACACGTTTGAGAGTACCGTACAGGTGAGCAAGTTGCAAGAC
  CTCATCCACCGCAGCAAGATGGCCCGGTGCAGAGGACGGTTTGTCTGCCCAGTAATCCTGTTCAAGGGC
  AAGCACATTTGCAGGTCGGCCACACTGGCTGGATGGGGAGAGCTGTATGGACGCTCAGGCTACAACTAT
  TTTTTCTCAGGGGGTGCAGATGATGCCTGGGCAGATGTGGAGGACGTCACGGAGGAGGACTGTGCTCTT
  CGAAGTGGTGACACGCATCTTTTTGATAAGGTCAGAGGCTATGACATCAAGCTGCTTCGATACCTGTCA
  GTCAAATACATCTGTGACCTGATGGTGGAGAACAAGAAGGTGAAGTTTGGCATGAATGTAACCTCCTCT
  GAGAAGGTGGACAAAGCCCAGCGCTATGCCGACTTCACTCTCCTCTCCATCCCGTATCCAGGCTGTGAA
  TTTTTCAAGGAATATAAAGATCGGGATTACATGGCAGAAGGGCTCATATTTAACTGGAAGCAGGACTAC
  GTTGATGCCCCATTGAGCATCCCCGACTTCCTGACTCACTCTCTGAACATTGACTGGAGCCAGTATCAG
  TGTTGGGATCTGGTGCAACAAACACAAAACTACCTGAAGCTGCTGCTTTCCTTAGTTAACAGTGATGAT
  GACAGCGGGCTGCTGGTACACTGTATCTCAGGCTGGGATCGGACCCCCCTCTTCATCTCCCTCCTGCGC
  CTTTCCTTGTGGGCTGATGGGCTCATCCACACGTCCCTGAAGCCCACTGAGATCCTCTACCTCACTGTG
  GCCTATGACTGGTTCCTCTTCGGGCACATGTTGGTAGATCGGCTCAGCAAAGGGGAGGAGATTTTCTTC
  TTCTGCTTCAATTTTTTGAAGCATATTACCTCCGAGGAGTTCTCTGCTCTGAAGACCCAGAGGAGGAAG
  AGTTTGCCAGCCCGGGATGGAGGCTTCACCCTGGAAGACATCTGCATGCTGAGACGAAAGGACCGTGGC
  AGCACCACCAGCCTTGGCAGCGACTTCTCCCTGGTCATGGAGAGTTCCCCAGGAGCCACTGGGAGCTTC
  ACCTATGAGGCCGTGGAGCTGGTCCCAGCAGGAGCGCCAACTCAGGCAGCTTGGCTTGCAGCCCTGAGT
  GATCGAGAGACTCGGCTGCAGGAGGTGCGCTCAGCCTTCTTGGCTGCGTACAGCAGCACAGTGGGGCTT
  CGGGCAGTAGCCCCCAGTCCTTCCGGTGCCATCGGGGGCCTGCTGGAGCAATTTGCCCGTGGTGTTGGA
  CTCCGGAGCATCAGCAGCAATGCCTTGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_022485
• Insert Size: 1617 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022485.4
• RefSeq Size: 2200 bp
• RefSeq ORF: 1617 bp
• Locus ID: 64419
• UniProt ID: Q8NCE2
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome, Phosphatase, Secreted Protein
• MW: 60 kDa
• Gene Summary: This gene encodes a myotubularin-related protein. The encoded protein is a phosphoinositide phosphatase that specifically dephosphorylates phosphatidylinositol 3,5-biphosphate and phosphatidylinositol 3-phosphate. Mutations in this gene are correlated with autosomal dominant centronuclear myopathy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 18.[provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (3) lacks two alternate in-frame segments in the 3' coding region, compared to variant 2. The resulting protein (isoform 3) has a shorter C-terminus when it is compared to isoform 2.