MTMR14 (NM_022485) Human Tagged ORF Clone

CAT#: RC200809

MTMR14 (Myc-DDK-tagged)-Human myotubularin related protein 14 (MTMR14), transcript variant 3

Product Images

Circular map for RC200809

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  Western blot validation of overexpression lysate (Cat# LY411652) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200809 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MTMR14 protein (Cat# TP300809). The protein was produced from HEK293T cells transfected with MTMR14 cDNA clone (Cat# RC200809) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTMR14
• Synonyms: C3orf29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200809 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCGCTCGGGCCGCCGCCGCCGCTGCCTCGGCGGGGTCCTCGGCCTCTTCAGGCAACCAGCCGC
  CTCAGGAGCTGGGGCTTGGGGAGCTGCTGGAGGAGTTCTCCCGGACTCAGTACCGGGCCAAGGATGGCAG
  CGGGACCGGCGGCTCTAAGGTTGAGCGCATTGAGAAGAGATGTCTGGAGCTGTTTGGCCGAGACTACTGT
  TTCAGCGTGATTCCAAACACGAATGGGGATATCTGTGGCCACTATCCCCGGCACATCGTGTTCCTGGAGT
  ATGAGAGTTCTGAGAAGGAGAAAGACACGTTTGAGAGTACCGTACAGGTGAGCAAGTTGCAAGACCTCAT
  CCACCGCAGCAAGATGGCCCGGTGCAGAGGACGGTTTGTCTGCCCAGTAATCCTGTTCAAGGGCAAGCAC
  ATTTGCAGGTCGGCCACACTGGCTGGATGGGGAGAGCTGTATGGACGCTCAGGCTACAACTATTTTTTCT
  CAGGGGGTGCAGATGATGCCTGGGCAGATGTGGAGGACGTCACGGAGGAGGACTGTGCTCTTCGAAGTGG
  TGACACGCATCTTTTTGATAAGGTCAGAGGCTATGACATCAAGCTGCTTCGATACCTGTCAGTCAAATAC
  ATCTGTGACCTGATGGTGGAGAACAAGAAGGTGAAGTTTGGCATGAATGTAACCTCCTCTGAGAAGGTGG
  ACAAAGCCCAGCGCTATGCCGACTTCACTCTCCTCTCCATCCCGTATCCAGGCTGTGAATTTTTCAAGGA
  ATATAAAGATCGGGATTACATGGCAGAAGGGCTCATATTTAACTGGAAGCAGGACTACGTTGATGCCCCA
  TTGAGCATCCCCGACTTCCTGACTCACTCTCTGAACATTGACTGGAGCCAGTATCAGTGTTGGGATCTGG
  TGCAACAAACACAAAACTACCTGAAGCTGCTGCTTTCCTTAGTTAACAGTGATGATGACAGCGGGCTGCT
  GGTACACTGTATCTCAGGCTGGGATCGGACCCCCCTCTTCATCTCCCTCCTGCGCCTTTCCTTGTGGGCT
  GATGGGCTCATCCACACGTCCCTGAAGCCCACTGAGATCCTCTACCTCACTGTGGCCTATGACTGGTTCC
  TCTTCGGGCACATGTTGGTAGATCGGCTCAGCAAAGGGGAGGAGATTTTCTTCTTCTGCTTCAATTTTTT
  GAAGCATATTACCTCCGAGGAGTTCTCTGCTCTGAAGACCCAGAGGAGGAAGAGTTTGCCAGCCCGGGAT
  GGAGGCTTCACCCTGGAAGACATCTGCATGCTGAGACGAAAGGACCGTGGCAGCACCACCAGCCTTGGCA
  GCGACTTCTCCCTGGTCATGGAGAGTTCCCCAGGAGCCACTGGGAGCTTCACCTATGAGGCCGTGGAGCT
  GGTCCCAGCAGGAGCGCCAACTCAGGCAGCTTGGCTTGCAGCCCTGAGTGATCGAGAGACTCGGCTGCAG
  GAGGTGCGCTCAGCCTTCTTGGCTGCGTACAGCAGCACAGTGGGGCTTCGGGCAGTAGCCCCCAGTCCTT
  CCGGTGCCATCGGGGGCCTGCTGGAGCAATTTGCCCGTGGTGTTGGACTCCGGAGCATCAGCAGCAATGC
  CTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200809 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGARAAAAAASAGSSASSGNQPPQELGLGELLEEFSRTQYRAKDGSGTGGSKVERIEKRCLELFGRDYC
  FSVIPNTNGDICGHYPRHIVFLEYESSEKEKDTFESTVQVSKLQDLIHRSKMARCRGRFVCPVILFKGKH
  ICRSATLAGWGELYGRSGYNYFFSGGADDAWADVEDVTEEDCALRSGDTHLFDKVRGYDIKLLRYLSVKY
  ICDLMVENKKVKFGMNVTSSEKVDKAQRYADFTLLSIPYPGCEFFKEYKDRDYMAEGLIFNWKQDYVDAP
  LSIPDFLTHSLNIDWSQYQCWDLVQQTQNYLKLLLSLVNSDDDSGLLVHCISGWDRTPLFISLLRLSLWA
  DGLIHTSLKPTEILYLTVAYDWFLFGHMLVDRLSKGEEIFFFCFNFLKHITSEEFSALKTQRRKSLPARD
  GGFTLEDICMLRRKDRGSTTSLGSDFSLVMESSPGATGSFTYEAVELVPAGAPTQAAWLAALSDRETRLQ
  EVRSAFLAAYSSTVGLRAVAPSPSGAIGGLLEQFARGVGLRSISSNAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022485
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022485.5
• RefSeq Size: 2200 bp
• RefSeq ORF: 1617 bp
• Locus ID: 64419
• UniProt ID: Q8NCE2
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome, Phosphatase, Secreted Protein
• MW: 60 kDa
• Gene Summary: This gene encodes a myotubularin-related protein. The encoded protein is a phosphoinositide phosphatase that specifically dephosphorylates phosphatidylinositol 3,5-biphosphate and phosphatidylinositol 3-phosphate. Mutations in this gene are correlated with autosomal dominant centronuclear myopathy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 18.[provided by RefSeq, Apr 2010]