NCOA7 (NM_181782) Human Untagged Clone

CAT#: SC316973

NCOA7 (untagged)-Human nuclear receptor coactivator 7 (NCOA7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCOA7
• Synonyms: dJ187J11.3; ERAP140; ESNA1; Nbla00052; Nbla10993; NCOA7-AS; TLDC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316973 representing NM_181782.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATACCAAGGAAGAGAAGAAGGAACGGAAACAAAGTTATTTTGCTCGACTGAAAAAGAAAAAACAA
  GCCAAACAAAATGCAGAGACAGCCTCAGCTGTAGCTACAAGGACTCATACTGGGAAGGAAGATAATAAT
  ACAGTAGTTTTAGAGCCAGACAAGTGCAACATTGCTGTGGAAGAGGAATATATGACTGATGAGAAAAAA
  AAGAGAAAAAGTAATCAGTTAAAGGAGATCAGGCGTACAGAACTAAAGAGATATTATAGTATTGATGAC
  AATCAAAACAAAACACATGATAAAAAAGAGAAGAAGATGGTGGTTCAGAAGCCCCATGGGACTATGGAA
  TACACTGCTGGAAACCAGGACACCCTAAACTCCATAGCACTGAAATTTAACATCACTCCCAATAAATTG
  GTGGAACTGAATAAACTTTTCACACATACTATTGTTCCAGGCCAGGTCCTTTTTGTGCCAGATGCCAAC
  TCTCCTTCCAGTACCTTAAGGCTATCATCATCCAGTCCTGGTGCTACTGTCTCTCCTTCATCATCAGAT
  GCAGAATATGATAAATTGCCTGATGCTGACTTAGCACGAAAGGCCTTGAAACCCATTGAAAGAGTCTTA
  TCGTCTACTTCTGAAGAAGATGAGCCAGGTGTGGTGAAATTTTTAAAAATGAATTGTCGATACTTCACC
  GATGGAAAGGGTGTGGTTGGCGGTGTTATGATAGTGACTCCTAACAACATCATGTTTGACCCTCATAAA
  TCTGATCCTCTGGTTATTGAAAATGGGTGTGAGGAGTATGGTCTCATCTGCCCCATGGAAGAGGTTGTT
  TCCATTGCGCTCTACAATGACATTTCTCACATGAAGATCAAAGATGCCTTGCCATCTGACCTACCTCAG
  GATCTTTGTCCTCTGTACAGGCCTGGAGAATGGGAAGACCTGGCTTCAGAAAAGGATATCAACCCATTC
  AGTAAGTTCAAATCTATCAACAAGGAAAAACGACAGCAGAATGGAGAGAAAATTATGACTTCGGATTCC
  AGACCAATAGTACCTTTGGAGAAGTCCACAGGACATACACCTACAAAGCCCTCAGGCAGCTCTGTGTCA
  GAGAAATTAAAGAAACTGGACTCCTCTAGGGAGACATCCCATGGTTCTCCCACAGTGACTAAGCTCAGC
  AAGGAACCTTCCGACACTTCTTCTGCATTTGAATCTACAGCCAAAGAAAACTTTCTAGGGGAAGATGAT
  GATTTTGTTGACTTGGAAGAACTTTCTTCTCAAACTGGTGGTGGAATGCACAAAAAAGACACCTTGAAG
  GAGTGCCTTTCTCTTGACCCAGAGGAACGAAAGAAAGCTGAGTCACAAATAAACAATTCTGCCGTGGAA
  ATGCAGGTGCAGTCAGCCCTAGCCTTTTTGGGAACAGAGAATGATGTTGAACTGAAGGGGGCGCTAGAT
  TTAGAAACCTGTGAGAAGCAAGATATAATGCCAGAAGTGGACAAGCAGTCTGGTTCGCCAGAAAGCCGA
  GTAGAAAACACACTGAACATACATGAAGATTTAGATAAAGTTAAACTCATTGAATATTACCTGACTAAG
  AACAAAGAAGGGCCACAGGTATCTGAAAATTTGCAGAAAACAGAATTAAGTGATGGAAAAAGTATTGAA
  CCAGGGGGAATAGACATTACCCTTAGTAGTTCTCTTTCCCAGGCGGGTGATCCCATAACTGAGGGCAAT
  AAAGAGCCAGATAAGACCTGGGTGAAAAAGGGAGAGCCCCTCCCGGTAAAACTGAACTCTTCTACAGAA
  GCAAATGTGATTAAAGAGGCTCTAGACTCCTCTTTGGAATCTACTCTGGACAACAGCTGTCAAGGTGCA
  CAAATGGATAATAAATCTGAAGTTCAGTTGTGGCTGTTAAAGAGAATTCAGGTACCCATTGAAGATATA
  CTTCCTTCAAAAGAAGAAAAAAGCAAGACCCCACCCATGTTCCTGTGCATCAAAGTGGGAAAACCAATG
  AGAAAATCCTTTGCCACTCACACTGCAGCCATGGTCCAGCAGTACGGCAAACGGAGAAAGCAGCCAGAG
  TACTGGTTTGCTGTTCCTCGGGAGAGGGTGGATCATTTGTACACATTCTTTGTTCAGTGGTCTCCCGAT
  GTCTATGGAAAAGATGCCAAAGAGCAAGGCTTTGTGGTGGTGGAGAAGGAAGAACTGAACATGATTGAC
  AACTTCTTCAGTGAGCCAACAACCAAGAGCTGGGAGATCATCACTGTTGAAGAGGCAAAGCGCAGGAAG
  AGCACATGCAGCTACTATGAAGACGAGGACGAAGAGGTGCTGCCTGTCCTACGGCCCCACAGCGCGCTC
  CTGGAGAATATGCACATCGAGCAGCTGGCCCGACGCCTTCCTGCAAGGGTGCAAGGGTATCCATGGAGA
  CTGGCCTATAGCACGTTAGAGCACGGGACCAGCTTAAAGACGCTCTACCGGAAATCGGCATCACTAGAC
  AGTCCTGTCCTATTGGTCATCAAAGATATGGATAATCAGATTTTTGGAGCATATGCAACTCATCCTTTC
  AAGTTCAGTGACCACTATTATGGCACAGGCGAAACTTTTCTCTACACATTCAGCCCTCATTTTAAGGTC
  TTTAAGTGGAGTGGAGAAAATTCATACTTTATCAATGGAGACATAAGTTCTTTAGAACTTGGTGGTGGA
  GGGGGACGATTTGGTTTATGGCTAGATGCTGATTTATACCACGGACGAAGCAACTCTTGCAGCACTTTC
  AATAATGATATTCTTTCCAAAAAGGAAGACTTCATAGTTCAGGATCTGGAGGTGTGGGCATTTGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_181782
• Insert Size: 2829 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181782.4
• RefSeq Size: 6457 bp
• RefSeq ORF: 2829 bp
• Locus ID: 135112
• UniProt ID: Q8NI08
• Cytogenetics: 6q22.31-q22.32
• Protein Families: Druggable Genome
• MW: 106.2 kDa
• Gene Summary: Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) and variants 3 and 4 encode the same longest isoform (1).