NCOA7 (NM_181782) Human Tagged ORF Clone

CAT#: RC221673

NCOA7 (Myc-DDK-tagged)-Human nuclear receptor coactivator 7 (NCOA7), transcript variant 1

Product Images

Circular map for RC221673

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NCOA7 (Cat# RC221673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCOA7 (Cat# TA810478)(1:2000). Positive lysates LY405613 (100ug) and LC405613 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY405613) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221673 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NCOA7 protein (Cat# TP321673). The protein was produced from HEK293T cells transfected with NCOA7 cDNA clone (Cat# RC221673) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NCOA7
• Synonyms: dJ187J11.3; ERAP140; ESNA1; Nbla00052; Nbla10993; NCOA7-AS; TLDC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221673 representing NM_181782
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACCAAGGAAGAGAAGAAGGAACGGAAACAAAGTTATTTTGCTCGACTGAAAAAGAAAAAACAAG
  CCAAACAAAATGCAGAGACAGCCTCAGCTGTAGCTACAAGGACTCATACTGGGAAGGAAGATAATAATAC
  AGTAGTTTTAGAGCCAGACAAGTGCAACATTGCTGTGGAAGAGGAATATATGACTGATGAGAAAAAAAAG
  AGAAAAAGTAATCAGTTAAAGGAGATCAGGCGTACAGAACTAAAGAGATATTATAGTATTGATGACAATC
  AAAACAAAACACATGATAAAAAAGAGAAGAAGATGGTGGTTCAGAAGCCCCATGGGACTATGGAATACAC
  TGCTGGAAACCAGGACACCCTAAACTCCATAGCACTGAAATTTAACATCACTCCCAATAAATTGGTGGAA
  CTGAATAAACTTTTCACACATACTATTGTTCCAGGCCAGGTCCTTTTTGTGCCAGATGCCAACTCTCCTT
  CCAGTACCTTAAGGCTATCATCATCCAGTCCTGGTGCTACTGTCTCTCCTTCATCATCAGATGCAGAATA
  TGATAAATTGCCTGATGCTGACTTAGCACGAAAGGCCTTGAAACCCATTGAAAGAGTCTTATCGTCTACT
  TCTGAAGAAGATGAGCCAGGTGTGGTGAAATTTTTAAAAATGAATTGTCGATACTTCACCGATGGAAAGG
  GTGTGGTTGGCGGTGTTATGATAGTGACTCCTAACAACATCATGTTTGACCCTCATAAATCTGATCCTCT
  GGTTATTGAAAATGGGTGTGAGGAGTATGGTCTCATCTGCCCCATGGAAGAGGTTGTTTCCATTGCGCTC
  TACAATGACATTTCTCACATGAAGATCAAAGATGCCTTGCCATCTGACCTACCTCAGGATCTTTGTCCTC
  TGTACAGGCCTGGAGAATGGGAAGACCTGGCTTCAGAAAAGGATATCAACCCATTCAGTAAGTTCAAATC
  TATCAACAAGGAAAAACGACAGCAGAATGGAGAGAAAATTATGACTTCGGATTCCAGACCAATAGTACCT
  TTGGAGAAGTCCACAGGACATACACCTACAAAGCCCTCAGGCAGCTCTGTGTCAGAGAAATTAAAGAAAC
  TGGACTCCTCTAGGGAGACATCCCATGGTTCTCCCACAGTGACTAAGCTCAGCAAGGAACCTTCCGACAC
  TTCTTCTGCATTTGAATCTACAGCCAAAGAAAACTTTCTAGGGGAAGATGATGATTTTGTTGACTTGGAA
  GAACTTTCTTCTCAAACTGGTGGTGGAATGCACAAAAAAGACACCTTGAAGGAGTGCCTTTCTCTTGACC
  CAGAGGAACGAAAGAAAGCTGAGTCACAAATAAACAATTCTGCCGTGGAAATGCAGGTGCAGTCAGCCCT
  AGCCTTTTTGGGAACAGAGAATGATGTTGAACTGAAGGGGGCGCTAGATTTAGAAACCTGTGAGAAGCAA
  GATATAATGCCAGAAGTGGACAAGCAGTCTGGTTCGCCAGAAAGCCGAGTAGAAAACACACTGAACATAC
  ATGAAGATTTAGATAAAGTTAAACTCATTGAATATTACCTGACTAAGAACAAAGAAGGGCCACAGGTATC
  TGAAAATTTGCAGAAAACAGAATTAAGTGATGGAAAAAGTATTGAACCAGGGGGAATAGACATTACCCTT
  AGTAGTTCTCTTTCCCAGGCGGGTGATCCCATAACTGAGGGCAATAAAGAGCCAGATAAGACCTGGGTGA
  AAAAGGGAGAGCCCCTCCCGGTAAAACTGAACTCTTCTACAGAAGCAAATGTGATTAAAGAGGCTCTAGA
  CTCCTCTTTGGAATCTACTCTGGACAACAGCTGTCAAGGTGCACAAATGGATAATAAATCTGAAGTTCAG
  TTGTGGCTGTTAAAGAGAATTCAGGTACCCATTGAAGATATACTTCCTTCAAAAGAAGAAAAAAGCAAGA
  CCCCACCCATGTTCCTGTGCATCAAAGTGGGAAAACCAATGAGAAAATCCTTTGCCACTCACACTGCAGC
  CATGGTCCAGCAGTACGGCAAACGGAGAAAGCAGCCAGAGTACTGGTTTGCTGTTCCTCGGGAGAGGGTG
  GATCATTTGTACACATTCTTTGTTCAGTGGTCTCCCGATGTCTATGGAAAAGATGCCAAAGAGCAAGGCT
  TTGTGGTGGTGGAGAAGGAAGAACTGAACATGATTGACAACTTCTTCAGTGAGCCAACAACCAAGAGCTG
  GGAGATCATCACTGTTGAAGAGGCAAAGCGCAGGAAGAGCACATGCAGCTACTATGAAGACGAGGACGAA
  GAGGTGCTGCCTGTCCTACGGCCCCACAGCGCGCTCCTGGAGAATATGCACATCGAGCAGCTGGCCCGAC
  GCCTTCCTGCAAGGGTGCAAGGGTATCCATGGAGACTGGCCTATAGCACGTTAGAGCACGGGACCAGCTT
  AAAGACGCTCTACCGGAAATCGGCATCACTAGACAGTCCTGTCCTATTGGTCATCAAAGATATGGATAAT
  CAGATTTTTGGAGCATATGCAACTCATCCTTTCAAGTTCAGTGACCACTATTATGGCACAGGCGAAACTT
  TTCTCTACACATTCAGCCCTCATTTTAAGGTCTTTAAGTGGAGTGGAGAAAATTCATACTTTATCAATGG
  AGACATAAGTTCTTTAGAACTTGGTGGTGGAGGGGGACGATTTGGTTTATGGCTAGATGCTGATTTATAC
  CACGGACGAAGCAACTCTTGCAGCACTTTCAATAATGATATTCTTTCCAAAAAGGAAGACTTCATAGTTC
  AGGATCTGGAGGTGTGGGCATTTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221673 representing NM_181782
  Red=Cloning site Green=Tags(s)
  
  MDTKEEKKERKQSYFARLKKKKQAKQNAETASAVATRTHTGKEDNNTVVLEPDKCNIAVEEEYMTDEKKK
  RKSNQLKEIRRTELKRYYSIDDNQNKTHDKKEKKMVVQKPHGTMEYTAGNQDTLNSIALKFNITPNKLVE
  LNKLFTHTIVPGQVLFVPDANSPSSTLRLSSSSPGATVSPSSSDAEYDKLPDADLARKALKPIERVLSST
  SEEDEPGVVKFLKMNCRYFTDGKGVVGGVMIVTPNNIMFDPHKSDPLVIENGCEEYGLICPMEEVVSIAL
  YNDISHMKIKDALPSDLPQDLCPLYRPGEWEDLASEKDINPFSKFKSINKEKRQQNGEKIMTSDSRPIVP
  LEKSTGHTPTKPSGSSVSEKLKKLDSSRETSHGSPTVTKLSKEPSDTSSAFESTAKENFLGEDDDFVDLE
  ELSSQTGGGMHKKDTLKECLSLDPEERKKAESQINNSAVEMQVQSALAFLGTENDVELKGALDLETCEKQ
  DIMPEVDKQSGSPESRVENTLNIHEDLDKVKLIEYYLTKNKEGPQVSENLQKTELSDGKSIEPGGIDITL
  SSSLSQAGDPITEGNKEPDKTWVKKGEPLPVKLNSSTEANVIKEALDSSLESTLDNSCQGAQMDNKSEVQ
  LWLLKRIQVPIEDILPSKEEKSKTPPMFLCIKVGKPMRKSFATHTAAMVQQYGKRRKQPEYWFAVPRERV
  DHLYTFFVQWSPDVYGKDAKEQGFVVVEKEELNMIDNFFSEPTTKSWEIITVEEAKRRKSTCSYYEDEDE
  EVLPVLRPHSALLENMHIEQLARRLPARVQGYPWRLAYSTLEHGTSLKTLYRKSASLDSPVLLVIKDMDN
  QIFGAYATHPFKFSDHYYGTGETFLYTFSPHFKVFKWSGENSYFINGDISSLELGGGGGRFGLWLDADLY
  HGRSNSCSTFNNDILSKKEDFIVQDLEVWAFD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181782
• ORF Size: 2826 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181782.5
• RefSeq Size: 5550 bp
• RefSeq ORF: 2829 bp
• Locus ID: 135112
• UniProt ID: Q8NI08
• Cytogenetics: 6q22.31-q22.32
• Protein Families: Druggable Genome
• MW: 106 kDa
• Gene Summary: Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.[UniProtKB/Swiss-Prot Function]