Myosin IIIB (MYO3B) (NM_138995) Human Untagged Clone

CAT#: SC315329

MYO3B (untagged)-Human myosin IIIB (MYO3B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Myosin IIIB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138995 edited
  CATCAATGAAACATCTGTATGGATTATTTCACTATAATCCTATGATGCTTGGACTTGAAT
  CACTTCCAGATCCCACAGACACCTGGGAAATTATAGAGACCATTGGTAAAGGCACCTATG
  GCAAAGTCTACAAGGTAACTAACAAGAGAGATGGGAGCCTGGCTGCAGTGAAAATTCTGG
  ATCCAGTCAGTGATATGGATGAAGAAATTGAGGCAGAATACAACATTTTGCAGTTCCTTC
  CTAATCATCCCAATGTTGTAAAGTTTTATGGGATGTTTTACAAAGCGGATCACTGTGTAG
  GGGGACAGCTGTGGCTGGTCCTGGAGCTGTGTAATGGGGGCTCAGTCACTGAGCTTGTCA
  AAGGTCTACTCAGATGTGGCCAGCGGTTGGATGAAGCAATGATCTCATACATCTTGTACG
  GGGCCCTCTTGGGCCTTCAGCATTTGCACAACAACCGAATCATCCACCGTGATGTGAAGG
  GGAATAACATTCTTCTGACAACAGAAGGAGGAGTTAAGCTCGTTGACTTTGGTGTTTCAG
  CTCAACTCACCAGTACACGTCTGCGGAGAAACACATCTGTTGGCACCCCGTTCTGGATGG
  CCCCTGAGGTCATTGCCTGTGAGCAGCAGTATGACTCTTCCTATGACGCTCGCTGTGACG
  TCTGGTCCTTGGGGATCACAGCTATTGAACTGGGGGATGGAGACCCTCCCCTCTTTGACA
  TGCATCCTGTGAAAACACTCTTTAAGATTCCAAGAAATCCTCCACCTACTTTACTTCATC
  CAGAAAAATGGTGTGAAGAATTCAACCACTTTATTTCACAGTGTCTTATTAAGGATTTTG
  AAAGGCGACCTTCCGTCACACATCTCCTTGACCACCCATTTATTAAAGGAGTACATGGAA
  AAGTTCTGTTTCTGCAAAAACAGCTGGCCGAGGTTCTCCAAGACCAGAAGCATCAAAATC
  CTGTTGCTAAAACCAGGCATGAGAGGATGCATACCAGAAGACCTTATCATGTGGAAGATG
  CTGAAAAATACTGCCTTGAGGATGATTTGGTCAACCTAGAGGTTCTGGATGAGGATACAA
  TTATCCATCAGTTGCAGAAACGTTATGCAGACTTGCTAATTTACACATATGTTGGAGACA
  TCTTAATTGCCTTAAACCCCTTCCAGAATCTAAGCATATACTCTCCACAGTTTTCCAGAC
  TTTATCATGGGGTGAAACGTGCCTCCAATCCCCCCCACATATTTGCATCAGCAGATGCTG
  CTTACCAGTGCATGGTTACTCTCAGCAAAGACCAGTGCATTGTCATCAGCGGAGAGAGTG
  GCTCTGGGAAGACAGAAAGCGCCCACCTGATTGTTCAGCATTTGACTTTCTTGGGAAAGG
  CCAATAATCAGACCTTGAGAGAGAAAATTCTACAAGTCAACTCCCTGGTGGAAGCCTTTG
  GGAACTCATGCACTGCCATCAATGACAACTCGAGCCGTTTTGGAAAATATCTGGAAATGA
  TGTTTACACCAACTGGAGTTGTGATGGGGGCAAGAATCTCTGAATATCTCCTGGAAAAAT
  CCAGAGTTATAAAACAGGCAGCGAGAGAGAAAAATTTTCATATATTTTACTATATTTATG
  CTGGTCTTCATCACCAAAAGAAGCTTTCTGATTTCAGACTTCCTGAGGAAAAACCTCCTA
  GGTACATAGCTGATGAAACTGGAAGGGTGATGCACGACATAACTTCCAAGGAGTCTTACA
  GAAGACAATTCGAAGCAATTCAGCATTGCTTCAGGATTATAGGGTTCACGGACAAAGAGG
  TGCACTCAGTGTACAGAATTTTGGCTGGGATTTTGAATATTGGGAACATTGAGTTCGCAG
  CTATTTCCTCTCAACATCAGACTGATAAAAGTGAGGTGCCCAATGCTGAAGCTTTGCAAA
  ATGCTGCCTCTGTTCTGTGCATTAGCCCTGAAGAGCTCCAGGAGGCCCTCACCTCCCACT
  GTGTGGTCACCCGGGGCGAGACCATCATCCGTGCCAACACTGTAGACAGGGCTGCGGACG
  TTCGAGACGCCATGTCCAAAGCCCTGTATGGGAGGCTCTTCAGCTGGATTGTGAATCGCA
  TTAATACACTCCTGCAGCCAGACGAAAACATATGTAGTGCAGGAGGTGGAATGAATGTGG
  GGATCTTGGATATCTTTGGATTCGAGAATTTTCAGAGAAATTCATTTGAGCAGCTCTGCA
  TAAACATCGCCAATGAGCAAATCCAGTACTATTTCAATCAGCATGTTTTTGCTCTTGAGC
  AGATGGAATATCAGAATGAAGGCATTGATGCTGTACCCGTGGAATATGAGGACAACCGCC
  CGCTCCTGGACATGTTCCTCCAGAAACCCCTGGGACTGCTTGCACTTTTGGATGAGGAAA
  GTCGGTTTCCCCAAGCAACTGACCAGACCCTGGTTGATAAATTTGAAGATAATCTACGAT
  GCAAATACTTCTGGAGGCCCAAAGGAGTGGAACTGTGCTTTGGCATTCAGCATTATGCTG
  GAAAGGTATTATATGATGCTTCTGGGGTTCTTGAGAAAAATAGAGACACTCTCCCTGCCG
  ATGTGGTTGTGGTCCTGAGAACGTCAGAAAACAAGCTTCTTCAGCAGCTCTTCTCAATCC
  CTCTGACCAAAACAGGTAATTTGGCCCAGACAAGAGCTAGGATAACAGTGGCCTCAAGTT
  CTTTGCCTCCACATTTCAGTGCTGGGAAAGCCAAGGTGGACACTCTGGAGGTGATACGGC
  ATCCGGAAGAAACCACCAACATGAAGAGGCAAACTGTGGCTTCTTACTTCCGGTATTCTC
  TGATGGACCTGCTCTCCAAAATGGTGGTTGGACAGCCCCACTTTGTGCGCTGCATTAAAC
  CCAATGATGACCGAGAGGCCCTGCAGTTCTCTCGAGAGAGGGTGCTGGCCCAGCTCCGCT
  CCACAGGGATTCTGGAGACAGTCAGCATCCGCCGCCAGGGCTATTCCCACCGCATCCTTT
  TTGAAGAATTTGTGAAAAGGTATTATTACTTGGCATTCACAGCACATCAAACACCTCTTG
  CTAGCAAAGAGAGCTGTGTGGCTATCTTGGAAAAGTCCAGATTAGATCACTGGGTGCTGG
  GAAAAACAAAGGTTTTTCTCAAATATTACCATGTTGAGCAATTAAATTTGCTGCTTCGAG
  AAGTCATAGGCAGAGTGGTTGTGCTGCAGGCATATACCAAGGGGTGGCTTGGAGCCAGGA
  GATACAAAAAGGTCAGAGAGAAGAGAGAGAAGGGAGCCATTGCCATCCAGTCAGCCTGGA
  GAGGATATGATGCTCGGAGGAAATTTAAGAAAATAAGCAACAGAAGGAATGAGTCTGCTG
  CTCATAATCAAGCAGGGGACACTTCAAACCAAAGCAGTGGGCCACATTCCCCCGTCGCAG
  CAGGTACGAGGGGAAGTGCCGAGGTTCAAGACTGCAGCGAGCCTGGTGACCATAAAGTTC
  TCAGGGGCTCTGTACATCGTAGGAGCCATTCACAAGCAGAATCCAACAATGGCCGTACAC
  AGACTTCAAGCAACTCTCCTGCTGTCACAGAGAAAAATGGGCATTCACAAGCCCAGAGTT
  CTCCAAAAGGGTGCGATATCTTCGCAGGACATGCAAACAAGCACTCGGTTTCTGGGACTG
  ATTTGCTGTCTTCTCGGATATGCCATCCTGCTCCAGATCAGCAAGGATTGAGTCTCTGGG
  GAGCCCCTCAAAAGCCTGGTTCAGAAAATGGTCTTGCACAGAAGCATCGAACACCTCGCC
  GACGATGTCAGCAGCCCAAAATGCTGAGTAGCCCTGAGGACACCATGTACTATAACCAGT
  TAAATGGAACTCTAGAATATCAAGGGAGCAAGAGGAAGCCAAGAAAACTTGGCCAAATCA
  AAGTACTTGATGGGGAAGATGAATATTACAAATCTCTGTCACCAGTGGACTGTATCCCTG
  AGGAGAACAACTCAGCCCACCCTTCCTTTTTTTCTTCATCCTCAAAAGGAGACTCTTTTG
  CTCAACATTAAATTGTGCTTCCTAACCCTAAATCTGTCCAGAGTAGGAACATTCATGGTA
  ATCGACTGTCTGTCATTGCGTAAGAAAGCACTGATATGGGGTCAGCTTCTTTGGACATAT
  GGTCCATGCCTGAACCTTACTGAACCACTTGCAGATTCCAAAACATCTTATCCTATCCTC
  TACCACTCTCCCACATGTGTTGTGCGGCCTGAGCTGGGCGCTGCCTTCCTTTCTCATCCC
  ATGGGGCCCTGTGGGACACTGAGAACACCTTTACAATAGTTTAAACAGTCATTCATGCCC
  CCAGTGTCTAGGAAGATAACAGCCAGTCTCACCCCAGTCTAATCATGGACCCTGATAATA
  TTGCTTGATTTTTCCTATCAAGTTACTTTTCAATCCATTCAGAATCTGCCCCAGTGGAGA
  CCCAGGAGTTCCTTTCCTGCACTCTTCTCCATCCTCCCACCTTTGCTGGGCTTTTCTATC
  ACTCCCACCTCCCCCAGAGTCAGGGCTCCATTGCTGAGTGCCCCATCCTGGAGGATTGGC
  CCCAAGATCTCCTAGAACAGGATAATTGCCTGTGTTTAGGCAGATAGGCCTAAATCTTTC
  AGATTCTTTCTACAAGGCAAATAACCCCTCTCTTGTTAATTATGATGCTGAGAAAGCCTC
  TGTCTCTTTATTTCACCTTGCCAAGACACCCCCCACAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138995 unedited
  CTACACATTGTACACGACTTACTATAGGGCGGCTCGCGAGGATATGCACGAGGCACTCAA
  TGAAACATCTGTACGGTACTTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACT
  TCCAGATCCCACAGACACCTGGGAAATTATAGAGACCATTGGTAAAGGCACCTATGGCAA
  AGTCTACAAGGTAACTAACAAGAGAGATGGGAGCCTGGCTGCAGTGAAAATTCTGGATCC
  AGTCAGTGATATGGATGAAGAAATTGAGGCAGAATACAACATTTTGCAGTTCCTTCCTAA
  TCATCCCAATGTTGTAAAGTTTTATGGGATGTTTTACAAAGCGGATCACTGTGTAGGGGG
  ACAGCTGTGGCTGGTCCTGGAGCTGTGTAATGGGGGCTCAGTCACTGAGCTTGTCAAAGG
  TCTACTCAGATGTGGCCAGCGGTTGGATGAAGCAATGATCTCATACATCTTGTACGGGGC
  CCTCTTGGGCCTTCAGCATTTGCACAACAACCGAATCATCCACCGTGATGTGAAGGGGAA
  TAACATTCTTCTGACAACAGAAGGAGGAGTTAAGCTCGTTGACTTTGGTGTTTCAGCTCA
  ACTCACCAGTACACGTCTGCGGAGAAACACATCTGTTGGCACCCCGTTCTGGATGGCCCC
  TGAGGTCATTGCCTGTGAGCAGCAGTATGACTCTTCCTATGACGCTCGCTGTGACGTCTG
  GTCCTTGGGGATCACAGCTATTGAACTGGGGGATGGAGACCCTCCCTCTTTGACATGCAT
  CCTGTGAAAACACTCTTTAGATTCCAAGAAATCTCCACCTACTTTACTCATCAGAAAATG
  GTGTGAGATCACCACTTTATTTCACAGTGTTCTTATTAAGATTTTGAAGGCGACCTTCCG
  TCA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_138995 unedited
  CCCGGGGGATTTGCCCATCCTGCTCCATAAGCAGGNATTGATCTCTGGGGAGCCCCTCAA
  AACCTGGTTCAGAAAAGGTCTTGCACAGAAGCATCGAACACCTCGCCGACGATGTCAGCA
  GCCCAAAATGCTGAGTAGCCCTGAGGACACCATGTACTATAACCAGTTAAATGGAACTCT
  AGAATATCAAGGGAGCAAGAGGAAGCCAAGAAAACTTGGCCAAATCAAAGTACTTGATGG
  GGAAGATGAATATTACAAATCTCTGTCACCAGTGGACTGTATCCCTGAGGAGAACAACTC
  AGCCCACCCTTCCTTTTTTTCTTCATCCTCAAAAGGAGACTCTTTTGCTCAACATTAAAT
  TGTGCTTCCTAACCCTAAATCTGTCCAGAGTAGGAACATTCATGGTAATCGACTGTCTGT
  CATTGCGTAAGAAAGCACTGATATGGGGTCAGCTTCTTTGGACATATGGTCCATGCCTGA
  ACCTTACTGAACCACTTGCAGATTCCAAAACATCTTATCCTATCCTCTACCACTCTCCCA
  CATGTGTTGTGCGGCCTGAGCTGGGCGCTGCCTTCCTTTCTCATCCCTGGGGCCCTGTGG
  GACACTGAGAACACCTTTACAATAGTTTAAACAGTCATTCATGCCCCCAGTGTCTAGGAA
  GATAACAGCCAGTCTCACCCCAGTCTAATCATGGACCCTGATAATATTGCTTGATTTTTC
  CTATCAAGTTACTTTTCAATCCATTCAGAATCTGCCCCAGTGGAGACCCAGGAGTTCCTT
  TCCTGCACTCTTCTCCATCCTCCCACCTTTGCTGGGCTTTTCTATCACTCCCACCTCCCC
  CCAGATCAGGGCTCCATTGCTGAGTGCC
  
• Restriction Sites: Please inquire
• ACCN: NM_138995
• Insert Size: 4800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138995.1, NP_620482.1
• RefSeq Size: 5560 bp
• RefSeq ORF: 3837 bp
• Locus ID: 140469
• UniProt ID: Q8WXR4
• Cytogenetics: 2q31.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes one of the class III myosins. Myosins are ATPases, activated by actin, that move along actin filaments in the cell. This class of myosins are characterized by an amino-terminal kinase domain and shown to be present in photoreceptors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (2) encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.