Myosin IIIB (MYO3B) (NM_138995) Human Tagged ORF Clone

CAT#: RG218508

MYO3B (tGFP-tagged) - Human myosin IIIB (MYO3B), transcript variant 2

Product Images

Circular map for RG218508

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Myosin IIIB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218508 representing NM_138995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACATCTGTATGGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTTCCAGATCCCA
  CAGACACCTGGGAAATTATAGAGACCATTGGTAAAGGCACCTATGGCAAAGTCTACAAGGTAACTAACAA
  GAGAGATGGGAGCCTGGCTGCAGTGAAAATTCTGGATCCAGTCAGTGATATGGATGAAGAAATTGAGGCA
  GAATACAACATTTTGCAGTTCCTTCCTAATCATCCCAATGTTGTAAAGTTTTATGGGATGTTTTACAAAG
  CGGATCACTGTGTAGGGGGACAGCTGTGGCTGGTCCTGGAGCTGTGTAATGGGGGCTCAGTCACTGAGCT
  TGTCAAAGGTCTACTCAGATGTGGCCAGCGGTTGGATGAAGCAATGATCTCATACATCTTGTACGGGGCC
  CTCTTGGGCCTTCAGCATTTGCACAACAACCGAATCATCCACCGTGATGTGAAGGGGAATAACATTCTTC
  TGACAACAGAAGGAGGAGTTAAGCTCGTTGACTTTGGTGTTTCAGCTCAACTCACCAGTACACGTCTGCG
  GAGAAACACATCTGTTGGCACCCCATTCTGGATGGCCCCTGAGGTCATTGCCTGTGAGCAGCAGTATGAC
  TCTTCCTATGACGCTCGCTGTGACGTCTGGTCCTTGGGGATCACAGCTATTGAACTGGGGGATGGAGACC
  CTCCCCTCTTTGACATGCATCCTGTGAAAACACTCTTTAAGATTCCAAGAAATCCTCCACCTACTTTACT
  TCATCCAGAAAAATGGTGTGAAGAATTCAACCACTTTATTTCACAGTGTCTTATTAAGGATTTTGAAAGG
  CGACCTTCCGTCACACATCTCCTTGACCACCCATTTATTAAAGGAGTACATGGAAAAGTTCTGTTTCTGC
  AAAAACAGCTGGCCAAGGTTCTCCAAGACCAGAAGCATCAAAATCCTGTTGCTAAAACCAGGCATGAGAG
  GATGCATACCAGAAGACCTTATCATGTGGAAGATGCTGAAAAATACTGCCTTGAGGATGATTTGGTCAAC
  CTAGAGGTTCTGGATGAGGATACAATTATCCATCAGTTGCAGAAACGTTATGCAGACTTGCTAATTTACA
  CATATGTTGGAGACATCTTAATTGCCTTAAACCCCTTCCAGAATCTAAGCATATACTCTCCACAGTTTTC
  CAGACTTTATCATGGGGTGAAACGCGCCTCCAATCCCCCCCACATATTTGCATCAGCAGATGCTGCTTAC
  CAGTGCATGGTTACTCTCAGCAAAGACCAGTGCATTGTCATCAGCGGAGAGAGTGGCTCTGGGAAGACAG
  AAAGCGCCCACCTGATTGTTCAACATTTGACTTTCTTGGGAAAGGCCAATAATCAGACCTTGAGAGAGAA
  AATTCTACAAGTCAACTCCCTGGTGGAAGCCTTTGGGAACTCATGCACTGCCATCAATGACAACTCGAGC
  CGTTTTGGAAAATATCTGGAAATGATGTTTACACCAACTGGAGTTGTGATGGGGGCAAGAATCTCTGAAT
  ATCTCCTGGAAAAATCCAGAGTTATAAAACAGGCAGCGAGAGAGAAAAATTTTCATATATTTTACTATAT
  TTATGCTGGTCTTCATCACCAAAAGAAGCTTTCTGATTTCAGACTTCCTGAGGAAAAACCTCCTAGGTAC
  ATAGCTGATGAAACTGGAAGGGTGATGCACGACATAACTTCCAAGGAGTCTTACAGAAGACAATTCGAAG
  CAATTCAGCATTGCTTCAGGATTATAGGGTTCACGGACAAAGAGGTGCACTCAGTGTACAGAATTTTGGC
  TGGGATTTTGAATATTGGGAACATTGAGTTCGCAGCTATTTCCTCTCAACATCAGACTGATAAAAGTGAG
  GTGCCCAATGCTGAAGCTTTGCAAAATGCTGCCTCTGTTCTGTGCATTAGCCCTGAAGAGCTCCAGGAGG
  CCCTCACCTCCCACTGTGTGGTCACCCGGGGCGAGACCATCATCCGTGCCAACACTGTAGACAGGGCTGC
  GGACGTTCGAGACGCCATGTCCAAAGCCCTGTATGGGAGGCTCTTCAGCTGGATTGTGAATCGCATTAAT
  ACACTCCTGCAGCCAGACGAAAACATATGTAGTGCAGGAGGTGGAATGAATGTGGGGATCTTGGATATCT
  TTGGATTCGAGAATTTTCAGAGAAATTCATTTGAGCAGCTCTGCATAAACATCGCCAATGAGCAAATCCA
  GTACTATTTCAATCAGCATGTTTTTGCTCTTGAGCAGATGGAATATCAGAATGAAGGCATTGATGCTGTA
  CCCGTGGAATATGAGGACAACCGCCCGCTCTTGGACATGTTCCTCCAGAAACCCCTGGGACTGCTTGCAC
  TTTTGGATGAGGAAAGTCGGTTTCCCCAAGCAACTGACCAGACCCTGGTTGATAAATTTGAAGATAATCT
  ACGATGCAAATACTTCTGGAGGCCCAAAGGAGTGGAACTGTGCTTTGGCATTCAGCATTATGCTGGAAAG
  GTATTATATGATGCTTCTGGGGTTCTTGAGAAAAATAGAGACACTCTCCCTGCCGATGTGGTTGTGGTCC
  TGAGAACGTCAGAAAACATGCTTCTTCAGCAGCTCTTCTCAATCCCTCTGACCAAAACAGGTAATTTGGC
  CCAGACAAGAGCTAGGATAACAGTGGCCTCAAGTTCTTTGCCTCCACATTTCAGTGCTGGGAAAGCCAAG
  GTGGACACTCTGGAGGTGATACGGCATCCGGAAGAAACCACCAACATGAAGAGGCAAACTGTGGCTTCTT
  ACTTCCGGTATTCTCTGATGGACCTGCTCTCCAAAATGGTGGTTGGACAGCCCCACTTTGTGCGCTGCAT
  TAAACCCAATGATGACCGAGAGGCCCTGCAGTTCTCTCGAGAGAGGGTGCTGGCCCAGCTCCGCTCCACA
  GGGATTCTGGAGACAGTCAGCATCCGCCGCCAGGGCTATTCCCACCGCATCCTTTTTGAAGAATTTGTGA
  AAAGGTATTATTACTTGGCATTCACAGCACATCAAACACCTCTTGCTAGCAAAGAGAGCTGTGTGGCTAT
  CTTGGAAAAGTCCAGATTAGATCACTGGGTGCTGGGAAAAACAAAGGTTTTTCTCAAATATTACCATGTT
  GAGCAATTAAATTTGCTGCTTCGAGAAGTCATAGGCAGAGTGGTTGTGCTGCAGGCATATACCAAGGGGT
  GGCTTGGAGCCAGGAGATACAAAAAGGTCAGAGAGAAGAGAGAGAAGGGAGCCATTGCCATCCAGTCAGC
  CTGGAGAGGATATGATGCTCGGAGGAAATTTAAGAAAATAAGCAACAGAAGGAATGAGTCTGCTGCTCAT
  AATCAAGCAGGGGACACTTCAAACCAAAGCAGTGGGCCACATTCCCCCGTCGCAGCAGGTACGAGGGGAA
  GTGCCGAGGTTCAAGACTGCAGCGAGCCTGGTGACCATAAAGTTCTCAGGGGCTCTGTACATCGTAGGAG
  CCATTCACAAGCAGAATCCAACAATGGCCGTACACAGACTTCAAGCAACTCTCCTGCTGTCACAGAGAAA
  AATGGGCATTCACAAGCCCAGAGTTCTCCAAAAGGGTGCGATATCTTCGCAGGACATGCAAACAAGCACT
  CGGTTTCTGGGACTGATTTGCTGTCTTCTCGGATATGCCATCCTGCTCCAGATCAGCAAGGATTGAGTCT
  CTGGGGAGCCCCTCAAAAGCCTGGTTCAGAAAATGGTCTTGCACAGAAGCATCGAACACCTCGCCGACGA
  TGTCAGCAGCCCAAAATGCTGAGTAGCCCTGAGGACACCATGTACTATAACCAGTTAAATGGAACTCTAG
  AATATCAAGGGAGCAAGAGGAAGCCAAGAAAACTTGGCCAAATCAAAGTACTTGATGGGGAAGATGAATA
  TTACAAATCTCTGTCACCAGTGGACTGTATCCCTGAGGAGAACAACTCAGCCCACCCTTCCTTTTTTTCT
  TCATCCTCAAAAGGAGACTCTTTTGCTCAACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218508 representing NM_138995
  Red=Cloning site Green=Tags(s)
  
  MKHLYGLFHYNPMMLGLESLPDPTDTWEIIETIGKGTYGKVYKVTNKRDGSLAAVKILDPVSDMDEEIEA
  EYNILQFLPNHPNVVKFYGMFYKADHCVGGQLWLVLELCNGGSVTELVKGLLRCGQRLDEAMISYILYGA
  LLGLQHLHNNRIIHRDVKGNNILLTTEGGVKLVDFGVSAQLTSTRLRRNTSVGTPFWMAPEVIACEQQYD
  SSYDARCDVWSLGITAIELGDGDPPLFDMHPVKTLFKIPRNPPPTLLHPEKWCEEFNHFISQCLIKDFER
  RPSVTHLLDHPFIKGVHGKVLFLQKQLAKVLQDQKHQNPVAKTRHERMHTRRPYHVEDAEKYCLEDDLVN
  LEVLDEDTIIHQLQKRYADLLIYTYVGDILIALNPFQNLSIYSPQFSRLYHGVKRASNPPHIFASADAAY
  QCMVTLSKDQCIVISGESGSGKTESAHLIVQHLTFLGKANNQTLREKILQVNSLVEAFGNSCTAINDNSS
  RFGKYLEMMFTPTGVVMGARISEYLLEKSRVIKQAAREKNFHIFYYIYAGLHHQKKLSDFRLPEEKPPRY
  IADETGRVMHDITSKESYRRQFEAIQHCFRIIGFTDKEVHSVYRILAGILNIGNIEFAAISSQHQTDKSE
  VPNAEALQNAASVLCISPEELQEALTSHCVVTRGETIIRANTVDRAADVRDAMSKALYGRLFSWIVNRIN
  TLLQPDENICSAGGGMNVGILDIFGFENFQRNSFEQLCINIANEQIQYYFNQHVFALEQMEYQNEGIDAV
  PVEYEDNRPLLDMFLQKPLGLLALLDEESRFPQATDQTLVDKFEDNLRCKYFWRPKGVELCFGIQHYAGK
  VLYDASGVLEKNRDTLPADVVVVLRTSENMLLQQLFSIPLTKTGNLAQTRARITVASSSLPPHFSAGKAK
  VDTLEVIRHPEETTNMKRQTVASYFRYSLMDLLSKMVVGQPHFVRCIKPNDDREALQFSRERVLAQLRST
  GILETVSIRRQGYSHRILFEEFVKRYYYLAFTAHQTPLASKESCVAILEKSRLDHWVLGKTKVFLKYYHV
  EQLNLLLREVIGRVVVLQAYTKGWLGARRYKKVREKREKGAIAIQSAWRGYDARRKFKKISNRRNESAAH
  NQAGDTSNQSSGPHSPVAAGTRGSAEVQDCSEPGDHKVLRGSVHRRSHSQAESNNGRTQTSSNSPAVTEK
  NGHSQAQSSPKGCDIFAGHANKHSVSGTDLLSSRICHPAPDQQGLSLWGAPQKPGSENGLAQKHRTPRRR
  CQQPKMLSSPEDTMYYNQLNGTLEYQGSKRKPRKLGQIKVLDGEDEYYKSLSPVDCIPEENNSAHPSFFS
  SSSKGDSFAQH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138995
• ORF Size: 4023 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138995.2, NP_620482.2
• RefSeq Size: 5529 bp
• RefSeq ORF: 4026 bp
• Locus ID: 140469
• UniProt ID: Q8WXR4
• Cytogenetics: 2q31.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes one of the class III myosins. Myosins are ATPases, activated by actin, that move along actin filaments in the cell. This class of myosins are characterized by an amino-terminal kinase domain and shown to be present in photoreceptors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]