Myosin IIIB (MYO3B) (NM_138995) Human Untagged Clone

CAT#: SC323595

MYO3B (untagged)-Kinase deficient mutant (K44M) of Human myosin IIIB (MYO3B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Myosin IIIB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_138995, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAACATCTGTATGGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTTCCAGATCCCA
  CAGACACCTGGGAAATTATAGAGACCATTGGTAAAGGCACCTATGGCAAAGTCTACAAGGTAACTAACAA
  GAGAGATGGGAGCCTGGCTGCAGTGAAAATTCTGGATCCAGTCAGTGATATGGATGAAGAAATTGAGGCA
  GAATACAACATTTTGCAGTTCCTTCCTAATCATCCCAATGTTGTAAAGTTTTATGGGATGTTTTACAAAG
  CGGATCACTGTGTAGGGGGACAGCTGTGGCTGGTCCTGGAGCTGTGTAATGGGGGCTCAGTCACTGAGCT
  TGTCAAAGGTCTACTCAGATGTGGCCAGCGGTTGGATGAAGCAATGATCTCATACATCTTGTACGGGGCC
  CTCTTGGGCCTTCAGCATTTGCACAACAACCGAATCATCCACCGTGATGTGAAGGGGAATAACATTCTTC
  TGACAACAGAAGGAGGAGTTAAGCTCGTTGACTTTGGTGTTTCAGCTCAACTCACCAGTACACGTCTGCG
  GAGAAACACATCTGTTGGCACCCCGTTCTGGATGGCCCCTGAGGTCATTGCCTGTGAGCAGCAGTATGAC
  TCTTCCTATGACGCTCGCTGTGACGTCTGGTCCTTGGGGATCACAGCTATTGAACTGGGGGATGGAGACC
  CTCCCCTCTTTGACATGCATCCTGTGAAAACACTCTTTAAGATTCCAAGAAATCCTCCACCTACTTTACT
  TCATCCAGAAAAATGGTGTGAAGAATTCAACCACTTTATTTCACAGTGTCTTATTAAGGATTTTGAAAGG
  CGACCTTCCGTCACACATCTCCTTGACCACCCATTTATTAAAGGAGTACATGGAAAAGTTCTGTTTCTGC
  AAAAACAGCTGGCCAAGGTTCTCCAAGACCAGAAGCATCAAAATCCTGTTGCTAAAACCAGGCATGAGAG
  GATGCATACCAGAAGACCTTATCATGTGGAAGATGCTGAAAAATACTGCCTTGAGGATGATTTGGTCAAC
  CTAGAGGTTCTGGATGAGGATACAATTATCCATCAGTTGCAGAAACGTTATGCAGACTTGCTAATTTACA
  CATATGTTGGAGACATCTTAATTGCCTTAAACCCCTTCCAGAATCTAAGCATATACTCTCCACAGTTTTC
  CAGACTTTATCATGGGGTGAAACGCGCCTCCAATCCCCCCCACATATTTGCATCAGCAGATGCTGCTTAC
  CAGTGCATGGTTACTCTCAGCAAAGACCAGTGCATTGTCATCAGCGGAGAGAGTGGCTCTGGGAAGACAG
  AAAGCGCCCACCTGATTGTTCAGCATTTGACTTTCTTGGGAAAGGCCAATAATCAGACCTTGAGAGAGAA
  AATTCTACAAGTCAACTCCCTGGTGGAAGCCTTTGGGAACTCATGCACTGCCATCAATGACAACTCGAGC
  CGTTTTGGAAAATATCTGGAAATGATGTTTACACCAACTGGAGTTGTGATGGGGGCAAGAATCTCTGAAT
  ATCTCCTGGAAAAATCCAGAGTTATAAAACAGGCAGCGAGAGAGAAAAATTTTCATATATTTTACTATAT
  TTATGCTGGTCTTCATCACCAAAAGAAGCTTTCTGATTTCAGACTTCCTGAGGAAAAACCTCCTAGGTAC
  ATAGCTGATGAAACTGGAAGGGTGATGCACGACATAACTTCCAAGGAGTCTTACAGAAGACAATTCGAAG
  CAATTCAGCATTGCTTCAGGATTATAGGGTTCACGGACAAAGAGGTGCACTCAGTGTACAGAATTTTGGC
  TGGGATTTTGAATATTGGGAACATTGAGTTCGCAGCTATTTCCTCTCAACATCAGACTGATAAAAGTGAG
  GTGCCCAATGCTGAAGCTTTGCAAAATGCTGCCTCTGTTCTGTGCATTAGCCCTGAAGAGCTCCAGGAGG
  CCCTCACCTCCCACTGTGTGGTCACCCGGGGCGAGACCATCATCCGTGCCAACACTGTAGACAGGGCTGC
  GGACGTTCGAGACGCCATGTCCAAAGCCCTGTATGGGAGGCTCTTCAGCTGGATTGTGAATCGCATTAAT
  ACACTCCTGCAGCCAGACGAAAACATATGTAGTGCAGGAGGTGGAATGAATGTGGGGATCTTGGATATCT
  TTGGATTCGAGAATTTTCAGAGAAATTCATTTGAGCAGCTCTGCATAAACATCGCCAATGAGCAAATCCA
  GTACTATTTCAATCAGCATGTTTTTGCTCTTGAGCAGATGGAATATCAGAATGAAGGCATTGATGCTGTA
  CCCGTGGAATATGAGGACAACCGCCCGCTCTTGGACATGTTCCTCCAGAAACCCCTGGGACTGCTTGCAC
  TTTTGGATGAGGAAAGTCGGTTTCCCCAAGCAACTGACCAGACCCTGGTTGATAAATTTGAAGATAATCT
  ACGATGCAAATACTTCTGGAGGCCCAAAGGAGTGGAACTGTGCTTTGGCATTCAGCATTATGCTGGAAAG
  GTATTATATGATGCTTCTGGGGTTCTTGAGAAAAATAGAGACACTCTCCCTGCCGATGTGGTTGTGGTCC
  TGAGAACGTCAGAAAACAAGCTTCTTCAGCAGCTCTTCTCAATCCCTCTGACCAAAACAGGTAATTTGGC
  CCAGACAAGAGCTAGGATAACAGTGGCCTCAAGTTCTTTGCCTCCACATTTCAGTGCTGGGAAAGCCAAG
  GTGGACACTCTGGAGGTGATACGGCATCCGGAAGAAACCACCAACATGAAGAGGCAAACTGTGGCTTCTT
  ACTTCCGGTATTCTCTGATGGACCTGCTCTCCAAAATGGTGGTTGGACAGCCCCACTTTGTGCGCTGCAT
  TAAACCCAATGATGACCGAGAGGCCCTGCAGTTCTCTCGAGAGAGGGTGCTGGCCCAGCTCCGCTCCACA
  GGGATTCTGGAGACAGTCAGCATCCGCCGCCAGGGCTATTCCCACCGCATCCTTTTTGAAGAATTTGTGA
  AAAGGTATTATTACTTGGCATTCACAGCACATCAAACACCTCTTGCTAGCAAAGAGAGCTGTGTGGCTAT
  CTTGGAAAAGTCCAGATTAGATCACTGGGTACTGGGAAAAACAAAGGTTTTTCTCAAATATTACCATGTT
  GAGCAATTAAATTTGCTGCTTCGAGAAGTCATAGGCAGAGTGGTTGTGCTGCAGGCATATACCAAGGGGT
  GGCTTGGAGCCAGGAGATACAAAAGGGTCAGAGAGAAGAGAGAGAAGGGAGCCATTGCCATCCAGTCAGC
  CTGGAGAGGATATGATGCTCGGAGGAAATTTAAGAAAATAAGCAACAGAAGGAATGAGTCTGCTGCTCAT
  AATCAAGCAGGGGACACTTCAAACCAAAGCAGTGGGCCACATTCCCCCGTCGCAGCAGGTACGAGGGGAA
  GTGCCGAGGTTCAAGACTGCAGCGAGCCTGGTGACCATAAAGTTCTCAGGGGCTCTGTACATCGTAGGAG
  CCATTCACAAGCAGAATCCAACAATGGCCGTACACAGACTTCAAGCAACTCTCCTGCTGTCACAGAGAAA
  AATGGGCATTCACAAGCCCAGAGTTCTCCAAAAGGGTGCGATATCTTCGCAGGACATGCAAACAAGCACT
  CGGTTTCTGGGACTGATTTGCTGTCTTCTCGGATATGCCATCCTGCTCCAGATCAGCAAGGATTGAGTCT
  CTGGGGAGCCCCTCAAAAGCCTGGTTCAGAAAATGGTCTTGCACAGAAGCATCGAACACCTCGCCGACGA
  TGTCAGCAGCCCAAAATGCTGAGTAGCCCTGAGGACACCATGTACTATAACCAGTTAAATGGAACTCTAG
  AATATCAAGGGAGCAAGAGGAAGCCAAGAAAACTTGGCCAAATCAAAGTACTTGATGGGGAAGATGAATA
  TTACAAATCTCTGTCACCAGTGGACTGTATCCCTGAGGAGAACAACTCAGCCCACCCTTCCTTTTTTTCT
  TCATCCTCAAAAGGAGACTCTTTTGCTCAACATTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_138995 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCAACAAGGAAACAT
  CTGTATGGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTTCCAGATCCCACAGACACCT
  GGGAAATTATAGAGACCATTGGTAAAGGCACCTATGGCAAAGTCTACAAGGTAACTAACAAGAGAGATGG
  GAGCCTGGCTGCAGTGATGATTCTGGATCCAGTCAGTGATATGGATGAAGAAATTGAGGCAGAATACAAC
  ATTTTTGCAGTTCCTTCCTAATCATCCCAATGTTTGTAAAGTTTTTATGGGGATGGTTTTAACAAAGCCG
  GATCACTGTGTAGGGGGACCAGCTGTGGCTGGTCCTGAGCTTGTGTAATGGGGGGCTCAGTCACTGAGCT
  TGTCAAGGTCTACTTAAATGTGGCAGCCGGTTGATTGAAGCCATGATTCTAAACTTCTTGTCCGGGGCCC
  CTCTTGGCCCTTCAGCATTGGCACAACACGGATATATCCCACCTTTGGTTGAAGGGAAATAACATCTCTT
  CGGCACAACAAAGGGGAGATTAAGCTGTTGACTTGGGTTTCACGCTACACTCACAATACGTCTCTGCGGA
  AACACATGTGTGCCCCCGCTTTGAGAGGCCCTGAGCTATCTGTAGACAGATAGACTTCTCATAACTCCGT
  GAACTGGCCTGGATACAATTAATGGGAGGAACCCCTTGACGCCTGGAACCTGATTCGAATTCGCAAATAT
  TCGGAAGTGGATTTTCGATACGCTTAAGTAGACTTGGCACTTTGACAAATTAGACCTG
  
  Kinase domain sequence

  >SC323595 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ACKTTGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCAACAAGGAAACATCTGTAT
  GGATTATTTCACTATAATCCTATGATGCTTGGACTTGAATCACTTCCAGATCCCACAGACACCTGGGAAA
  TTATAGAGACCATTGGTAAAGGCACCTATGGCAAAGTCTACAAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_138995
• Insert Size: 4800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138995.1, NP_620482.1
• RefSeq Size: 5560 bp
• RefSeq ORF: 3837 bp
• Locus ID: 140469
• UniProt ID: Q8WXR4
• Cytogenetics: 2q31.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes one of the class III myosins. Myosins are ATPases, activated by actin, that move along actin filaments in the cell. This class of myosins are characterized by an amino-terminal kinase domain and shown to be present in photoreceptors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (2) encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.