PTPRN2 (NM_130842) Human Untagged Clone

CAT#: SC315054

PTPRN2 (untagged)-Human protein tyrosine phosphatase, receptor type, N polypeptide 2 (PTPRN2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPRN2
• Synonyms: IA-2beta; IAR; ICAAR; PTPRP; R-PTP-N2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315054 representing NM_130842.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGCCGCCGCTCCCGCTGCTGCTGCTGCTACTGCTGCTGCTGCCGCCACGCGTCCTGCCTGCCGCC
  CCTTCGTCCGTCCCCCGCGGCCGGCAGCTCCCGGGGCGTCTGGATGGAGTGTTTGGAAGGTGCCAGAAG
  GTTCCGGCAATGGACTTTTACCGCTACGAGGTGTCGCCCGTGGCCCTGCAGCGCCTGCGCGTGGCGTTG
  CAGAAGCTTTCCGGCACAGGTTTCACGTGGCAGGATGACTATACTCAGTATGTGATGGACCAGGAACTT
  GCAGACCTCCCGAAAACCTACCTGAGGCGTCCTGAAGCATCCAGCCCAGCCAGGCCCTCAAAACACAGC
  GTTGGCAGCGAGAGGAGGTACAGTCGGGAGGGCGGTGCTGCCCTGGCCAACGCCCTCCGACGCCACCTG
  CCCTTCCTGGAGGCCCTGTCCCAGGCCCCAGCCTCAGACGTGCTCGCCAGGACCCATACGGCGCAGGAC
  AGACCCCCCGCTGAGGGTGATGACCGCTTCTCCGAGAGCATCCTGACCTATGTGGCCCACACGTCTGCG
  CTGACCTACCCTCCCGGGTCCCGGACCCAGCTCCGCGAGGACCTCCTGCCGCGGACCCTCGGCCAGCTC
  CAGCCAGATGAGCTCAGCCCTAAGGTGGACAGTGGTGTGGACAGACACCATCTGATGGCGGCCCTCAGT
  GCCTATGCTGCCCAGAGGCCCCCAGCTCCCCCCGGGGAGGGCAGCCTGGAGCCACAGTACCTTCTGCGT
  GCACCCTCAAGAATGCCCAGGCCTTTGCTGGCACCAGCCGCCCCCCAGAAGTGGCCTTCACCTCTGGGA
  GATTCCGAAGACCCCTCCAGCACAGGCGATGGAGCACGGATTCATACCCTCCTGAAGGACCTGCAGAGG
  CAGCCGGCTGAGGTGAGGGGCCTGAGTGGCCTGGAGCTGGACGGCATGGCTGAGCTGATGGCTGGCCTG
  ATGCAAGGCGTGGACCATGGAGTAGCTCGAGGCAGCCCTGGGAGAGCGGCCCTGGGAGAGTCTGGAGAA
  CAGGCGGATGGCCCCAAGGCCACCCTCCGTGGAGACAGCTTTCCAGATGACGGAGTGCAGGACGACGAT
  GATAGACTTTACCAAGAGGTCCATCGTCTGAGTGCCACACTCGGGGGCCTCCTGCAGGACCACGGGTCT
  CGACTCTTACCTGGAGCCCTCCCCTTTGCAAGGCCCCTCGACATGGAGAGGAAGAAGTCCGAGCACCCT
  GAGTCTTCCCTGTCTTCAGAAGAGGAGACTGCCGGAGTGGAGAACGTCAAGAGCCAGACGTATTCCAAA
  GATCTGCTGGGGCAGCAGCCGCATTCGGAGCCCGGGGCCGCTGCGTTTGGGGAGCTCCAAAACCAGATG
  CCTGGGCCCTCGAAGGAGGAGCAGAGCCTTCCAGCGGGTGCTCAGGAGGCCCTCAGCGACGGCCTGCAA
  TTGGAGGTCCAGCCTTCCGAGGAAGAGGCGCGGGGCTACATCGTGACAGACAGAGACCCCCTGCGCCCC
  GAGGAAGGAAGGCGGCTGGTGGAGGACGTCGCCCGCCTCCTGCAGGTGCCCAGCAGTGCGTTCGCTGAC
  GTGGAGGTTCTCGGACCAGCAGTGACCTTCAAAGTGAGCGCCAATGTCCAAAACGTGACCACTGAGGAT
  GTGGAGAAGGCCACAGTTGACAACAAAGACAAACTGGAGGAAACCTCTGGACTGAAAATTCTTCAAACC
  GGAGTCGGGTCGAAAAGCAAACTCAAGTTCCTGCCTCCTCAGGCGGAGCAAGAAGACTCCACCAAGTTC
  ATCGCGCTCACCCTGGTCTCCCTCGCCTGCATCCTGGGCGTCCTCCTGGCCTCTGGCCTCATCTACTGC
  CTCCGCCATAGCTCTCAGCACAGGCTGAAGGAGAAGCTCTCGGGACTAGGGGGCGACCCAGGTGCAGAT
  GCCACTGCCGCCTACCAGGAGCTGTGCCGCCAGCGTATGGCCACGCGGCCACCAGACCGACCTGAGGGC
  CCGCACACGTCACGCATCAGCAGCGTCTCATCCCAGTTCAGCGACGGGCCGATCCCCAGCCCCTCCGCA
  CGCAGCAGCGCCTCATCCTGGTCCGAGGAGCCTGTGCAGTCCAACATGGACATCTCCACCGGCCACATG
  ATCCTGTCCTACATGGAGGACCACCTGAAGAACAAGAACCGGCTGGAGAAGGAGTGGGAAGCGCTGTGC
  GCCTACCAGGCGGAGCCCAACAGCTCGTTCGTGGCCCAGAGGGAGGAGAACGTGCCCAAGAACCGCTCC
  CTGGCCGTGCTGACCTATGACCACTCCCGGGTCCTGCTGAAGGCGGAGAACAGCCACAGCCACTCAGAC
  TACATCAACGCTAGCCCCATCATGGATCACGACCCGAGGAACCCCGCGTACATCGCCACCCAGGGACCG
  CTGCCCGCCACCGTGGCTGACTTTTGGCAGATGGTGTGGGAGAGCGGCTGCGTGGTGATCGTCATGCTG
  ACACCCCTCGCGGAGAACGGCGTCCGGCAGTGCTACCACTACTGGCCGGATGAAGGCTCCAATCTCTAC
  CACATCTATGAGGTGAACCTGGTCTCCGAGCACATCTGGTGTGAGGACTTCCTGGTGAGGAGCTTCTAT
  CTGAAGAACCTGCAGACCAACGAGACGCGCACCGTGACGCAGTTCCACTTCCTGAGTTGGTATGACCGA
  GGAGTCCCTTCCTCCTCAAGGTCCCTCCTGGACTTCCGCAGAAAAGTAAACAAGTGCTACAGGGGCCGT
  TCTTGTCCAATAATTGTTCATTGCAGTGACGGTGCAGGCCGGAGCGGCACCTACGTCCTGATCGACATG
  GTTCTCAACAAGATGGCCAAAGGTGCTAAAGAGATTGATATCGCAGCGACCCTGGAGCACTTGAGGGAC
  CAGAGACCCGGCATGGTCCAGACGAAGGAGCAGTTTGAGTTCGCGCTGACAGCCGTGGCTGAGGAGGTG
  AACGCCATCCTCAAGGCCCTTCCCCAGTGA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_130842
• Insert Size: 2997 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130842.3
• RefSeq Size: 4808 bp
• RefSeq ORF: 2997 bp
• Locus ID: 5799
• UniProt ID: Q92932
• Cytogenetics: 7q36.3
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Type I diabetes mellitus
• MW: 109.6 kDa
• Gene Summary: This gene encodes a protein with sequence similarity to receptor-like protein tyrosine phosphatases. However, tyrosine phosphatase activity has not been experimentally validated for this protein. Studies of the rat ortholog suggest that the encoded protein may instead function as a phosphatidylinositol phosphatase with the ability to dephosphorylate phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-diphosphate, and this function may be involved in the regulation of insulin secretion. This protein has been identified as an autoantigen in insulin-dependent diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.