PTPRN2 (NM_130842) Human Tagged ORF Clone

CAT#: RG213126

PTPRN2 (tGFP-tagged) - Human protein tyrosine phosphatase, receptor type, N polypeptide 2 (PTPRN2), transcript variant 2

Product Images

Circular map for RG213126

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRN2
• Synonyms: IA-2beta; IAR; ICAAR; PTPRP; R-PTP-N2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213126 representing NM_130842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCGCCGCTCCCGCTGCTGCTGCTGCTACTGCTGCTGCTGCCGCCACGCGTCCTGCCTGCCGCCC
  CTTCGTCCGTCCCCCGCGGCCGGCAGCTCCCGGGGCGTCTGGATGGAGTGTTTGGAAGGTGCCAGAAGGT
  TCCGGCAATGGACTTTTACCGCTACGAGGTGTCGCCCGTGGCCCTGCAGCGCCTGCGCGTGGCGTTGCAG
  AAGCTTTCCGGCACAGGTTTCACGTGGCAGGATGACTATACTCAGTATGTGATGGACCAGGAACTTGCAG
  ACCTCCCGAAAACCTACCTGAGGCGTCCTGAAGCATCCAGCCCAGCCAGGCCCTCAAAACACAGCGTTGG
  CAGCGAGAGGAGGTACAGTCGGGAGGGCGGTGCTGCCCTGGCCAACGCCCTCCGACGCCACCTGCCCTTC
  CTGGAGGCCCTGTCCCAGGCCCCAGCCTCAGACGTGCTCGCCAGGACCCATACGGCGCAGGACAGACCCC
  CCGCTGAGGGTGATGACCGCTTCTCCGAGAGCATCCTGACCTATGTGGCCCACACGTCTGCGCTGACCTA
  CCCTCCCGGGCCCCGGACCCAGCTCCGCGAGGACCTCCTGCCGCGGACCCTCGGCCAGCTCCAGCCAGAT
  GAGCTCAGCCCTAAGGTGGACAGTGGTGTGGACAGACACCATCTGATGGCGGCCCTCAGTGCCTATGCTG
  CCCAGAGGCCCCCAGCTCCCCCCGGGGAGGGCAGCCTGGAGCCACAGTACCTTCTGCGTGCACCCTCAAG
  AATGCCCAGGCCTTTGCTGGCACCAGCCGCCCCCCAGAAGTGGCCTTCACCTCTGGGAGATTCCGAAGAC
  CCCTCTAGCACAGGCGATGGAGCACGGATTCATACCCTCCTGAAGGACCTGCAGAGGCAGCCGGCTGAGG
  TGAGGGGCCTGAATGGCCTGGAGCTGGACGGCATGGCCGAGCTGATGGCTGGCCTGATGCAAGGCGTGGA
  CCATGGAGTAGCTCGAGGCAGCCCTGGGAGAGCGGCCCTGGGAGAGTCTGGAGAACAGGCGGATGGCCCC
  AAGGCCACCCTCCGTGGAGACAGCTTTCCAGATGACGGAGTGCAGGACGACGATGATAGACTTTACCAAG
  AGGTCCATCGTCTGAGTGCCACACTCGGGGGCCTCCTGCAGGACCACGGGTCTCGACTCTTACCTGGAGC
  CCTCCCCTTTGCAAGGCCCCTCGACATGGAGAGGAAGAAGTCCGAGCACCCTGAGTCTTCCCTGTCTTCA
  GAAGAGGAGACTGCCGGAGTGGAGAACGTCAAGAGCCAGACGTATTCCAAAGATCTGCTGGGGCAGCAGC
  CGCATTCGGAGCCCGGGGCCGCTGCGTTTGGGGAGCTCCAAAACCAGATGCCTGGGCCCTCGAAGGAGGA
  GCAGAGCCTTCCAGCGGGTGCTCAGGAGGCCCTCAGCGACGGCCTGCAATTGGAGGTCCAGCCTTCCGAG
  GAAGAGGCGCGGGGCTACATCGTGACAGACAGAGACCCCCTGCGCCCCGAGGAAGGAAGGCGGCTGGTGG
  AGGACGTCGCCCGCCTCCTGCAGGTGCCCAGCAGTGCGTTCGCTGACGTGGAGGTTCTCGGACCAGCAGT
  GACCTTCAAAGTGAGCGCCAATGTCCAAAACGTGACCACTGAGGATGTGGAGAAGGCCACAGTTGACAAC
  AAAGACAAACTGGAGGAAACCTCTGGACTGAAAATTCTTCAAACCGGAGTCGGGTCGAAAAGCAAACTCA
  AGTTCCTGCCTCCTCAGGCGGAGCAAGAAGACTCCACCAAGTTCATCGCGCTCACCCTGGTCTCCCTCGC
  CTGCATCCTGGGCGTCCTCCTGGCCTCTGGCCTCATCTACTGCCTCCGCCATAGCTCTCAGCACAGGCTG
  AAGGAGAAGCTCTCGGGACTAGGGGGCGACCCAGGTGCAGATGCCACTGCCGCCTACCAGGAGCTGTGCC
  GCCAGCGTATGGCCACGCGGCCACCAGACCGACCTGAGGGCCCGCACACGTCACGCATCAGCAGCGTCTC
  ATCCCAGTTCAGCGACGGGCCGATCCCCAGCCCCTCCGCACGCAGCAGCGCCTCATCCTGGTCCGAGGAG
  CCTGTGCAGTCCAACATGGACATCTCCACCGGCCACATGATCCTGTCCTACATGGAGGACCACCTGAAGA
  ACAAGAACCGGCTGGAGAAGGAGTGGGAAGCGCTGTGCGCCTACCAGGCGGAGCCCAACAGCTCGTTCGT
  GGCCCAGAGGGAGGAGAACGTGCCCAAGAACCGCTCCCTGGCTGTGCTGACCTATGACCACTCCCGGGTC
  CTGCTGAAGGCGGAGAACAGCCACAGCCACTCAGACTACATCAACGCTAGCCCCATCATGGATCACGACC
  CGAGGAACCCCGCGTACATCGCCACCCAGGGACCGCTGCCCGCCACCGTGGCTGACTTTTGGCAGATGGT
  GTGGGAGAGCGGCTGCGTGGTGATCGTCATGCTGACACCCCTCGCGGAGAACGGCGTCCGGCAGTGCTAC
  CACTACTGGCCGGATGAAGGCTCCAATCTCTACCACATCTATGAGGTGAACCTGGTCTCCGAGCACATCT
  GGTGTGAGGACTTCCTGGTGAGGAGCTTCTATCTGAAGAACCTGCAGACCAACGAGACGCGCACCGTGAC
  GCAGTTCCACTTCCTGAGTTGGTATGACCGAGGAGTCCCTTCCTCCTCAAGGTCCCTCCTGGACTTCCGC
  AGAAAAGTAAACAAGTGCTACAGGGGCCGTTCTTGTCCAATAATTGTTCATTGCAGTGACGGTGCAGGCC
  GGAGCGGCACCTACGTCCTGATCGACATGGTTCTCAACAAGATGGCCAAAGGTGCTAAAGAGATTGATAT
  CGCAGCGACCCTGGAGCACTTGAGGGACCAGAGACCCGGCATGGTCCAGACGAAGGAGCAGTTTGAGTTC
  GCGCTGACAGCCGTGGCTGAGGAGGTGAACGCCATCCTCAAGGCCCTTCCCCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213126 representing NM_130842
  Red=Cloning site Green=Tags(s)
  
  MGPPLPLLLLLLLLLPPRVLPAAPSSVPRGRQLPGRLDGVFGRCQKVPAMDFYRYEVSPVALQRLRVALQ
  KLSGTGFTWQDDYTQYVMDQELADLPKTYLRRPEASSPARPSKHSVGSERRYSREGGAALANALRRHLPF
  LEALSQAPASDVLARTHTAQDRPPAEGDDRFSESILTYVAHTSALTYPPGPRTQLREDLLPRTLGQLQPD
  ELSPKVDSGVDRHHLMAALSAYAAQRPPAPPGEGSLEPQYLLRAPSRMPRPLLAPAAPQKWPSPLGDSED
  PSSTGDGARIHTLLKDLQRQPAEVRGLNGLELDGMAELMAGLMQGVDHGVARGSPGRAALGESGEQADGP
  KATLRGDSFPDDGVQDDDDRLYQEVHRLSATLGGLLQDHGSRLLPGALPFARPLDMERKKSEHPESSLSS
  EEETAGVENVKSQTYSKDLLGQQPHSEPGAAAFGELQNQMPGPSKEEQSLPAGAQEALSDGLQLEVQPSE
  EEARGYIVTDRDPLRPEEGRRLVEDVARLLQVPSSAFADVEVLGPAVTFKVSANVQNVTTEDVEKATVDN
  KDKLEETSGLKILQTGVGSKSKLKFLPPQAEQEDSTKFIALTLVSLACILGVLLASGLIYCLRHSSQHRL
  KEKLSGLGGDPGADATAAYQELCRQRMATRPPDRPEGPHTSRISSVSSQFSDGPIPSPSARSSASSWSEE
  PVQSNMDISTGHMILSYMEDHLKNKNRLEKEWEALCAYQAEPNSSFVAQREENVPKNRSLAVLTYDHSRV
  LLKAENSHSHSDYINASPIMDHDPRNPAYIATQGPLPATVADFWQMVWESGCVVIVMLTPLAENGVRQCY
  HYWPDEGSNLYHIYEVNLVSEHIWCEDFLVRSFYLKNLQTNETRTVTQFHFLSWYDRGVPSSSRSLLDFR
  RKVNKCYRGRSCPIIVHCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQFEF
  ALTAVAEEVNAILKALPQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130842
• ORF Size: 2994 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130842.1, NP_570857.1
• RefSeq Size: 4716 bp
• RefSeq ORF: 2997 bp
• Locus ID: 5799
• UniProt ID: Q92932
• Cytogenetics: 7q36.3
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Type I diabetes mellitus
• Gene Summary: This gene encodes a protein with sequence similarity to receptor-like protein tyrosine phosphatases. However, tyrosine phosphatase activity has not been experimentally validated for this protein. Studies of the rat ortholog suggest that the encoded protein may instead function as a phosphatidylinositol phosphatase with the ability to dephosphorylate phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-diphosphate, and this function may be involved in the regulation of insulin secretion. This protein has been identified as an autoantigen in insulin-dependent diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]