Semaphorin 3D (SEMA3D) (NM_152754) Human Untagged Clone

CAT#: SC314672

SEMA3D (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Semaphorin 3D
• Synonyms: coll-2; Sema-Z2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_152754 edited
  ATGAATGCTAATAAAGATGAAAGACTTAAAGCCAGAAGCCAAGATTTTCACCTTTTTCCT
  GCTTTGATGATGCTAAGCATGACCATGTTGTTTCTTCCAGTCACTGGCACTTTGAAGCAA
  AATATTCCAAGACTCAAGCTAACCTACAAAGACTTGCTGCTTTCAAATAGCTGTATTCCC
  TTTTTGGGTTCATCAGAAGGACTGGATTTTCAAACTCTTCTCTTAGATGAGGAAAGAGGC
  AGGCTGCTCTTGGGAGCCAAAGACCACATCTTTCTACTCAGTCTGGTTGACTTAAACAAA
  AATTTTAAGAAGATTTATTGGCCTGCTGCAAAGGAACGGGTGGAATTATGTAAATTAGCT
  GGGAAAGATGCCAATACAGAATGTGCAAATTTCATCAGAGTACTTCAGCCCTATAACAAA
  ACTCACATATATGTGTGTGGAACTGGAGCATTTCATCCAATATGTGGGTATATTGATCTT
  GGAGTCTACAAGGAGGATATTATATTCAAACTAGACACACATAATTTGGAGTCTGGCAGA
  CTGAAATGTCCTTTCGATCCTCAGCAGCCTTTTGCTTCAGTAATGACAGATGAGTACCTC
  TACTCTGGAACAGCTTCTGATTTCCTTGGCAAAGATACTGCATTCACTCGATCCCTTGGG
  CCTACTCATGACCACCACTACATCAGAACTGACATTTCAGAGCACTACTGGCTCAATGGA
  GCAAAATTTATTGGAACTTTCTTCATACCAGACACCTACAATCCAGATGATGATAAAATA
  TATTTCTTCTTTCGTGAATCATCTCAAGAAGGCAGTACCTCCGATAAAACCATCCTTTCT
  CGAGTTGGAAGAGTTTGTAAGAATGATGTAGGAGGACAACGCAGCCTGATAAACAAGTGG
  ACGACTTTTCTTAAGGCCAGACTGATTTGCTCAATTCCTGGAAGTGATGGGGCAGATACT
  TACTTTGATGAGCTTCAAGATATTTATTTACTCCCCACAAGAGATGAAAGAAATCCTGTA
  GTATATGGAGTCTTTACTACAACCAGCTCCATCTTCAAAGGCTCTGCTGTTTGTGTGTAT
  AGCATGGCTGACATCAGAGCAGTTTTTAATGGTCCATATGCTCATAAGGAAAGTGCAGAC
  CATCGTTGGGTGCAGTATGATGGGAGAATTCCTTATCCACGGCCTGGTACATGTCCAAGC
  AAAACCTATGACCCACTGATTAAGTCCACCCGAGATTTTCCAGATGATGTCATCAGTTTC
  ATAAAGCGGCACTCTGTGATGTATAAGTCCGTATACCCAGTTGCAGGAGGACCAACGTTC
  AAGAGAATCAATGTGGATTACAGACTGACACAGATAGTGGTGGATCATGTCATTGCAGAA
  GATGGCCAGTACGATGTAATGTTTCTTGGAACAGACATTGGAACTGTCCTCAAAGTTGTC
  AGCATTTCAAAGGAAAAGTGGAATATGGAAGAGGTAGTGCTGGAGGAGTTGCAGATATTC
  AAGCACTCATCAATCATCTTGAACATGG:AATTGTCTCTGAAGCAGCAACAATTGTACAT
  TGGTTCCCGAGATGGATTGGTTCAGCTCTCCTTGCACAGATGCGACACTTATGGGAAAGC
  TTGCGCAGACTGTTGTCTTGCCAGAGACCCCTACTGTGCCTGGGATGGAAATGCATGCTC
  TCGATATGCTCCTACTTCTAAAAGGAGAGCTAGACGCCAAGATGTAAAATATGGCGACCC
  AATCACCCAGTGCTGGGACATCGAAGACAGCATTAGTCATGAAACTGCTGATGAAAAGGT
  GATTTTTGGCATTGAATTTAACTCAACCTTTCTGGAATGTATACCTAAATCCCAACAAGC
  AACTATTAAATGGTATATCCAGAGGTCAGGGGATGAGCATCGAGAGGAGTTGAAGCCCGA
  TGAAAGAATCATCAAAACGGAATATGGGCTACTGATTCGAAGTTTGCAGAAGAAGGATTC
  TGGGATGTATTACTGCAAAGCCCAGGAGCACACTTTCATCCACACCATAGTGAAGCTGAC
  TTTGAATGTCATTGAGAATGAACAGATGGAAAATACCCAGAGGGCAGAGCATGAGGAGGG
  GAAGGTCAAGGATCTATTGGCTGAGTCACGGTTGAGATACAAAGACTACATCCAAATCCT
  TAGCAGCCCAAACTTCAGCCTCGACCAGTACTGCGAACAGATGTGGCACAGGGAGAAGCG
  GAGACAGAGAAACAAGGGGGGCCCAAAGTGGAAGCACATGCAGGAAATGAAGAAGAAACG
  AAATCGAAGACATCACAGAGACCTGGATGAGCTCCCTAGAGCTGTAGCCACGTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_152754
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_152754.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152754.2, NP_689967.2
• RefSeq Size: 6276 bp
• RefSeq ORF: 2334 bp
• Locus ID: 223117
• UniProt ID: O95025
• Cytogenetics: 7q21.11
• Domains: IG, PSI
• Protein Families: Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin like domain and a C-terminal basic domain. The protein encoded by this gene binds neuropilin and plays an important role in cardiovascular development. [provided by RefSeq, Aug 2016]