DCAMKL1 (DCLK1) (NM_004734) Human Untagged Clone

CAT#: SC314561

DCLK1 (untagged)-Human doublecortin-like kinase 1 (DCLK1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCAMKL1
• Synonyms: CL1; CLICK1; DCAMKL1; DCDC3A; DCLK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004734 edited
  ATGTCCTTCGGCAGAGACATGGAGCTGGAGCACTTCGACGAGCGGGATAAGGCGCAGAGA
  TACAGCCGAGGGTCGCGGGTGAACGGCCTGCCGAGCCCGACGCACAGCGCCCACTGCAGC
  TTCTACCGCACCCGCACGCTGCAGACGCTCAGCTCCGAGAAGAAGGCCAAGAAAGTTCGT
  TTCTATCGAAACGGAGATCGATACTTCAAAGGGATTGTGTATGCCATCTCCCCAGACCGG
  TTCCGATCTTTTGAGGCCCTGCTGGCTGATTTGACCCGAACTCTGTCGGATAACGTGAAT
  TTGCCCCAGGGAGTGAGAACAATCTACACCATTGATGGGCTCAAGAAGATTTCCAGCCTG
  GACCAACTGGTGGAAGGAGAGAGTTATGTATGTGGCTCCATAGAGCCCTTCAAGAAACTG
  GAGTACACCAAGAATGTGAACCCCAACTGGTCGGTGAACGTCAAGACCACCTCGGCTTCT
  CGGGCAGTGTCTTCACTGGCCACTGCCAAAGGAAGCCCTTCAGAGGTGCGAGAGAATAAG
  GATTTCATTCGGCCCAAGCTGGTCACCATCATCAGAAGTGGCGTGAAGCCACGGAAAGCT
  GTCAGGATTCTGCTGAACAAGAAAACGGCTCATTCCTTTGAGCAGGTCCTCACCGATATC
  ACCGATGCCATCAAGCTGGACTCGGGAGTGGTGAAACGCCTGTACACGTTGGATGGGAAA
  CAGGTGATGTGCCTTCAGGACTTTTTTGGTGATGATGACATTTTTATTGCATGTGGACCG
  GAGAAGTTCCGTTACCAGGATGATTTCTTGCTAGATGAAAGTGAATGTCGAGTGGTAAAG
  TCCACTTCTTACACCAAAATAGCTTCATCATCCCGCAGGAGCACCACCAAGAGCCCAGGA
  CCGTCCAGGCGTAGCAAGTCCCCTGCCTCCACCAGCTCAGTTAATGGAACCCCTGGTAGT
  CAGCTCTCTACTCCGCGCTCAGGCAAGTCGCCAAGCCCATCACCCACCAGCCCAGGAAGC
  CTGCGGAAGCAGAGGAGCTCTCAGCATGGCGGCTCCTCTACGTCACTTGCGTCCACCAAA
  GTCTGCAGCTCGATGGATGAGAACGATGGCCCTGGAGAAGAAGTGTCGGAGGAAGGCTTC
  CAGATTCCAGCTACAATAACAGAACGATATAAAGTCGGAAGAACAATAGGAGATGGAAAT
  TTTGCTGTTGTCAAGGAATGTGTAGAAAGATCGACTGCTAGAGAGTACGCTCTGAAAATT
  ATCAAGAAAAGCAAATGTCGAGGCAAAGAGCACATGATCCAGAATGAAGTGTCTATTTTA
  AGAAGAGTGAAGCATCCCAATATCGTTCTTCTGATTGAGGAGATGGATGTGCCAACTGAA
  CTGTATCTTGTCATGGAATTAGTAAAGGGGGGAGACCTTTTTGATGCCATTACTTCCACT
  AACAAATACACCGAGAGAGACGCCAGTGGGATGCTGTACAACCTAGCCAGCGCCATCAAA
  TACCTGCATAGCCTGAACATCGTCCACCGTGATATCAAGCCAGAGAACCTGCTGGTGTAT
  GAGCACCAAGATGGCAGCAAATCACTGAAGCTGGGTGACTTTGGACTGGCCACCATTGTA
  GACGGCCCCCTGTACACAGTCTGTGGCACCCCAACATACGTGGCTCCAGAAATCATTGCA
  GAGACTGGATACGGCCTCAAGGTGGACATCTGGGCAGCAGGTGTAATCACTTATATCCTG
  CTGTGTGGTTTCCCTCCATTCCGTGGAAGTGGTGATGACCAGGAGGTGCTTTTTGATCAG
  ATTTTGATGGGGCAGGTGGACTTTCCTTCTCCATACTGGGATAATGTTTCCGATTCTGCA
  AAGGAGCTCATTACCATGATGCTGTTGGTCGATGTAGATCAGCGATTTTCTGCTGTTCAA
  GTACTTGAGCATCCCTGGGTTAATGATGATGGCCTCCCAGAAAATGAACATCAGCTGTCA
  GTAGCTGGAAAGATAAAGAAGCATTTCAACACAGGCCCCAAGCCGAATAGCACAGCAGCT
  GGAGTTTCTGTCATAGCACTGGACCACGGGTTTACCATCAAGAGATCAGGGTCTTTGGAC
  TACTACCAGCAACCAGGAATGTATTGGATAAGACCACCGCTCTTGATAAGGAGAGGCAGG
  TTTTCCGACGAAGACGCAACCAGGATGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_004734
• Insert Size: 2200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004734.2, NP_004725.1
• RefSeq Size: 5703 bp
• RefSeq ORF: 2190 bp
• Locus ID: 9201
• UniProt ID: O15075
• Cytogenetics: 13q13.3
• Domains: pkinase, TyrKc, S_TKc, DCX
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (1, also known as DCLK-long-B, DCK-alpha1 or KIAA0369AS) is produced from the 5' promoter. It encodes the isoform 1, which includes two doublecortin domains, a serine/proline-rich domain and a protein kinase domain. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.